Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A an...Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.展开更多
文摘Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.
