Introduction: Mycoplasmas are bacteria commonly found in the commensal flora of humans and can occasionally be pathogenic. The population and the geographical area determine their susceptibility to various antibiotics...Introduction: Mycoplasmas are bacteria commonly found in the commensal flora of humans and can occasionally be pathogenic. The population and the geographical area determine their susceptibility to various antibiotics. Methodology: We carried out a cross-sectional study from January to June 2022 at the Douala General Hospital. All women who were sexually active and willing to participate were included. Endo-cervical swabbing was used to collect the samples. Culture and antibiotic susceptibility testing were performed in a liquid medium using Mycoplasma-SystemPlus Gallery (Liofilchem). All samples that degraded urea (threshold > 10<sup>3</sup> for Ureaplasma urealyticum) and arginine (threshold > 10<sup>4</sup> for Mycoplasma hominis) were deemed positive. Results: A total of 107 women with a median age of 33 ± 8.3 years and a predominance of 30-40 years (41.1%) were included. The frequency of mycoplasma infection was 73.8% with 34.1%, 11.4% and 54.4% for Ureaplasma urealyticum, Mycoplasma hominis and co-infection respectively. Resistance rates to Minocycline were low, 1.3% for Ureaplasma urealyticum and 3.7% for co-infection;for Pefloxacin the resistance rates were 3.7% (Ureaplasma urealyticum, Mycoplasma hominis) and 22.8% (co-infection). Ureaplasma urealyticum had a resistance rate of 3.7% for Erythromycin, 1.3% for Clarithromycin and 5% for Azithromycin. There was no significant correlation between risk factors and infection. Vaginal cleansing and the development of resistance in mycoplasma infection were found to be significantly correlated (OR = 6.915 [1.52 - 31.55];(p = 0.013)). Conclusion: Minocycline was the most active antibiotic and Ureaplasma urealyticum was the species with the lowest rate of resistance. Antibiotic resistance was more common in co-infected people than mono-infected. Antibiotic resistance was independently correlated with vaginal douching.展开更多
Rates of urogenital mycoplasmosis associated with Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum have been reported numerous times, and frequently show a wide range of finding...Rates of urogenital mycoplasmosis associated with Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum have been reported numerous times, and frequently show a wide range of findings. Differing diagnostic techniques, population targeting, temporal and spatial data collection, and coincident infections make the conclusions from these analyses difficult to compare. We generated a single data set including the infection rate, geographic location, year, study population, diagnostic method, and clinical signs for these organisms by performing literature searches with the species names and compiling the findings. Studies focusing on basic research or reporting clinical surveys where these criteria were not reported were excluded. A statistical analysis of the dataset parameters found that: diagnostic method does not significantly correlate with positive specimen rate but does correlate with the year of publication, and the number of publications correlated significantly with year, indicating that this topic is of growing interest. Further analysis indicated that Ureaplasma species infection rate is significantly higher in pregnant women across all studies. Associations with distinct clinical presentation could not be made on datasets assembled across studies due to the number of confounding variables presented in each. The generated data set represents a large amount of temporal, geographic, and clinical data that can be utilized in future communications.展开更多
文摘Introduction: Mycoplasmas are bacteria commonly found in the commensal flora of humans and can occasionally be pathogenic. The population and the geographical area determine their susceptibility to various antibiotics. Methodology: We carried out a cross-sectional study from January to June 2022 at the Douala General Hospital. All women who were sexually active and willing to participate were included. Endo-cervical swabbing was used to collect the samples. Culture and antibiotic susceptibility testing were performed in a liquid medium using Mycoplasma-SystemPlus Gallery (Liofilchem). All samples that degraded urea (threshold > 10<sup>3</sup> for Ureaplasma urealyticum) and arginine (threshold > 10<sup>4</sup> for Mycoplasma hominis) were deemed positive. Results: A total of 107 women with a median age of 33 ± 8.3 years and a predominance of 30-40 years (41.1%) were included. The frequency of mycoplasma infection was 73.8% with 34.1%, 11.4% and 54.4% for Ureaplasma urealyticum, Mycoplasma hominis and co-infection respectively. Resistance rates to Minocycline were low, 1.3% for Ureaplasma urealyticum and 3.7% for co-infection;for Pefloxacin the resistance rates were 3.7% (Ureaplasma urealyticum, Mycoplasma hominis) and 22.8% (co-infection). Ureaplasma urealyticum had a resistance rate of 3.7% for Erythromycin, 1.3% for Clarithromycin and 5% for Azithromycin. There was no significant correlation between risk factors and infection. Vaginal cleansing and the development of resistance in mycoplasma infection were found to be significantly correlated (OR = 6.915 [1.52 - 31.55];(p = 0.013)). Conclusion: Minocycline was the most active antibiotic and Ureaplasma urealyticum was the species with the lowest rate of resistance. Antibiotic resistance was more common in co-infected people than mono-infected. Antibiotic resistance was independently correlated with vaginal douching.
文摘Rates of urogenital mycoplasmosis associated with Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum have been reported numerous times, and frequently show a wide range of findings. Differing diagnostic techniques, population targeting, temporal and spatial data collection, and coincident infections make the conclusions from these analyses difficult to compare. We generated a single data set including the infection rate, geographic location, year, study population, diagnostic method, and clinical signs for these organisms by performing literature searches with the species names and compiling the findings. Studies focusing on basic research or reporting clinical surveys where these criteria were not reported were excluded. A statistical analysis of the dataset parameters found that: diagnostic method does not significantly correlate with positive specimen rate but does correlate with the year of publication, and the number of publications correlated significantly with year, indicating that this topic is of growing interest. Further analysis indicated that Ureaplasma species infection rate is significantly higher in pregnant women across all studies. Associations with distinct clinical presentation could not be made on datasets assembled across studies due to the number of confounding variables presented in each. The generated data set represents a large amount of temporal, geographic, and clinical data that can be utilized in future communications.
