A redox active medicinal plant and L-leucine mixture (HLM) was investigated in subjects with established osteoarthritis of the knee in a multi-center, rando- m-ized, placebo-controlled, double-blind clinical trial. A ...A redox active medicinal plant and L-leucine mixture (HLM) was investigated in subjects with established osteoarthritis of the knee in a multi-center, rando- m-ized, placebo-controlled, double-blind clinical trial. A total of 96 subjects with osteoarthritis were enrolled and randomized to either placebo (n = 38) or HLM treatment group (n = 38). The HLM group re- ceived a combination of Uncaria tomentosa (300 mg), Boswellia serrata (200 mg), Lepidium meyenii (1000 mg) and L-Leucine (700 mg) given as 3 capsules once a day. The placebo group received matching capsules with carboxymethylcellulose. The treatment period was 8 weeks, with assessments made at days 7, 14, 28 and 56. The primary outcome was reduction in total WOMAC score. VAS pain, tolerability, investigator assessments, use of rescue medication (acetominophen), and safety assessments of vital signs and laboratory assessments were included. Subject randomization was effective for age, gender and disease severity. In the placebo group 32/38 subjects completed the trial and for HLM 35/38. WOMAC scores (pain, stiffness, physical performance and total) steadily declined over the course of the 8 week study in both groups, but the magnitude was significantly greater for HLM (P < 0.05). Total WOMAC was reduced 46.5% for HLM and 25.4 % for placebo. VAS pain was reduced 21.8% in the placebo group (p < 0.002) but the changes were significantly greater (37.8% p < 0.03) with HLM treatment. Investigator’s global assessment rating of good-excellent was 24/35 (69%) for HLM and 14/32 (44%) for placebo (P = 0.05). Rescue medication consumption and tolerability were comparable for HLM and placebo. No safety issues were evident with either group. As expected a placebo effect was observed, nevertheless HLM was clearly more effective in relieving the symptoms of osteoarthritis. This HLM represents a safe and effective new approach to the management of osteoarthritis symptoms.展开更多
Uncaria tomentosa presents tomentum that resembles cat’s claws, hence its common name, is a plant that produces various secondary metabolites that are traditionally used in alternative medicine. The natural distribut...Uncaria tomentosa presents tomentum that resembles cat’s claws, hence its common name, is a plant that produces various secondary metabolites that are traditionally used in alternative medicine. The natural distribution of this species has been affected by indiscriminate harvesting from its habitat. In the present research, cryopreservation (liquid nitrogen, LN, -196°C) was evaluated as an option for ex situ conservation of this species. The following techniques were evaluated: vitrification and encapsulation-dehydration of apices, vitrification of cell suspensions, and seed desiccation and vitrification. Preculture conditions and exposure times to LS and PVS2 were evaluated. Apex survival was the highest (82%) with preculture in 0.25 M sucrose followed by incubation for 20 and 30 min in LS and PVS2, respectively, prior to cooling in LN. The encapsulation-dehydration technique was evaluated by using sucrose preculture and different capsule moisture contents. Survival of apices cooled in LN was not significantly different between treatments and varied from 31.8% to 52.9% for capsule moisture contents between 22.7% and 20.3%. For cell suspensions precultured in 0.5 M sucrose, cell multiplication and formation of calli with very good appearance were observed in 61.1% of the cultures following vitrification. For cryopreservation of seeds, germination was 89.5% using the desiccation technique and 67.6% to78.1% using vitrification.展开更多
目的:绒毛钩藤广泛分布于南美洲及中美洲部分雨林地区,具有抗氧化、抗炎、增强免疫和抑制肿瘤细胞增殖等多种药理活性,通过分析探讨其中药药性,以期增加其药用价值和开发前景。方法:文章通过检索中国知网、万方、维普、PubMed、Web of S...目的:绒毛钩藤广泛分布于南美洲及中美洲部分雨林地区,具有抗氧化、抗炎、增强免疫和抑制肿瘤细胞增殖等多种药理活性,通过分析探讨其中药药性,以期增加其药用价值和开发前景。方法:文章通过检索中国知网、万方、维普、PubMed、Web of Science和Scopus数据库对绒毛钩藤的科学研究文献进行筛选分类,遵循中医药理论,探讨明确绒毛钩藤的药用部位及中药药性。结果:绒毛钩藤以树皮和根皮入药,绒毛钩藤皮的中药药性为性寒,味辛、苦,归肝、肾、脾经。功能主治:祛风湿,止痹痛,清热解毒散结;主治风湿痹痛、关节肿胀、屈伸不利;癌症肿毒,外治热毒炽盛所致的口疮。用法用量:煎服,5~20 g。外用适量。使用注意:孕妇、儿童及肝肾功能损害者慎用。结论:通过研究赋予绒毛钩藤皮中药药性,为其临床应用与中药配伍提供理论依据,从而扩大中药资源。展开更多
文摘A redox active medicinal plant and L-leucine mixture (HLM) was investigated in subjects with established osteoarthritis of the knee in a multi-center, rando- m-ized, placebo-controlled, double-blind clinical trial. A total of 96 subjects with osteoarthritis were enrolled and randomized to either placebo (n = 38) or HLM treatment group (n = 38). The HLM group re- ceived a combination of Uncaria tomentosa (300 mg), Boswellia serrata (200 mg), Lepidium meyenii (1000 mg) and L-Leucine (700 mg) given as 3 capsules once a day. The placebo group received matching capsules with carboxymethylcellulose. The treatment period was 8 weeks, with assessments made at days 7, 14, 28 and 56. The primary outcome was reduction in total WOMAC score. VAS pain, tolerability, investigator assessments, use of rescue medication (acetominophen), and safety assessments of vital signs and laboratory assessments were included. Subject randomization was effective for age, gender and disease severity. In the placebo group 32/38 subjects completed the trial and for HLM 35/38. WOMAC scores (pain, stiffness, physical performance and total) steadily declined over the course of the 8 week study in both groups, but the magnitude was significantly greater for HLM (P < 0.05). Total WOMAC was reduced 46.5% for HLM and 25.4 % for placebo. VAS pain was reduced 21.8% in the placebo group (p < 0.002) but the changes were significantly greater (37.8% p < 0.03) with HLM treatment. Investigator’s global assessment rating of good-excellent was 24/35 (69%) for HLM and 14/32 (44%) for placebo (P = 0.05). Rescue medication consumption and tolerability were comparable for HLM and placebo. No safety issues were evident with either group. As expected a placebo effect was observed, nevertheless HLM was clearly more effective in relieving the symptoms of osteoarthritis. This HLM represents a safe and effective new approach to the management of osteoarthritis symptoms.
文摘Uncaria tomentosa presents tomentum that resembles cat’s claws, hence its common name, is a plant that produces various secondary metabolites that are traditionally used in alternative medicine. The natural distribution of this species has been affected by indiscriminate harvesting from its habitat. In the present research, cryopreservation (liquid nitrogen, LN, -196°C) was evaluated as an option for ex situ conservation of this species. The following techniques were evaluated: vitrification and encapsulation-dehydration of apices, vitrification of cell suspensions, and seed desiccation and vitrification. Preculture conditions and exposure times to LS and PVS2 were evaluated. Apex survival was the highest (82%) with preculture in 0.25 M sucrose followed by incubation for 20 and 30 min in LS and PVS2, respectively, prior to cooling in LN. The encapsulation-dehydration technique was evaluated by using sucrose preculture and different capsule moisture contents. Survival of apices cooled in LN was not significantly different between treatments and varied from 31.8% to 52.9% for capsule moisture contents between 22.7% and 20.3%. For cell suspensions precultured in 0.5 M sucrose, cell multiplication and formation of calli with very good appearance were observed in 61.1% of the cultures following vitrification. For cryopreservation of seeds, germination was 89.5% using the desiccation technique and 67.6% to78.1% using vitrification.
基金supported by grants from The National Natural Science Foundation of China (30930036)a research grant from Life Extension Foundation, USA and BM-A2 research grant from BioMarkerPharmaceuticals, Inc. USA~~
文摘目的:绒毛钩藤广泛分布于南美洲及中美洲部分雨林地区,具有抗氧化、抗炎、增强免疫和抑制肿瘤细胞增殖等多种药理活性,通过分析探讨其中药药性,以期增加其药用价值和开发前景。方法:文章通过检索中国知网、万方、维普、PubMed、Web of Science和Scopus数据库对绒毛钩藤的科学研究文献进行筛选分类,遵循中医药理论,探讨明确绒毛钩藤的药用部位及中药药性。结果:绒毛钩藤以树皮和根皮入药,绒毛钩藤皮的中药药性为性寒,味辛、苦,归肝、肾、脾经。功能主治:祛风湿,止痹痛,清热解毒散结;主治风湿痹痛、关节肿胀、屈伸不利;癌症肿毒,外治热毒炽盛所致的口疮。用法用量:煎服,5~20 g。外用适量。使用注意:孕妇、儿童及肝肾功能损害者慎用。结论:通过研究赋予绒毛钩藤皮中药药性,为其临床应用与中药配伍提供理论依据,从而扩大中药资源。
