Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial inf...Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial infections world widely and associated with important medical and financial implications. This retrospective study aimed to establish the bacterial profile of UTIs in Benin. Therefore, reported data were collected from 2003 to 2012 at Menontin’s Hospital in Cotonou and subjected to analyses. At the end of the study, fourteen different bacteria species were found to be associated with UTI cases in Benin. Escherichia coli was the most prevalent followed by Staphylococcus aureus. Results of the current study are helpful to the health care community of Benin in establishing the appropriate antibiotherapy for UTI patients’ treatment with respect organisms into circulation.展开更多
Objectives: To evaluate the efficacy, compliance, safety and economic cost for Fosfomycin trometamol and Nitofurantoin in uncomplicated lower urinary tract infections during pregnancy. Background: Nitofurantoin and Fo...Objectives: To evaluate the efficacy, compliance, safety and economic cost for Fosfomycin trometamol and Nitofurantoin in uncomplicated lower urinary tract infections during pregnancy. Background: Nitofurantoin and Fosfomycin trometamol are recommended as the first-line agents for treatment of urinary tract infections (UTIs) in the latest guidelines endorsed by the Infectious Diseases Society of America (IDSA) and the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). Fosfomycin is bactericidal and inhibits bacterial cell wall biogenesis and reduces bacterial adherence to uroepithelial cells. Fosfomycin has broad antibacterial activity against both Gram-positive and Gram-negative pathogens, as Escherichia coli, Escherichia faecalis, and various Gram-negatives like Citrobacter and Proteus. Both Nitofurantoin and Fosfomycin are category B in pregnancy. Patients and Methods: This study was conducted at Tanta University Hospitals in the period from June, 1, 2015 to January, 1, 2017. Patients were recruited from outpatient clinics of Obstetrics and Gynecology and Urology Departments presenting with asymptomatic bacteruria or cystitis. Patients were allocated randomly into 2 groups: group I (n = 50 cases) received Fosfomycin therapy and group II (n = 50 cases) received Nitofurantoin therapy (n = 50 cases). After treatment, evaluation of patient symptoms, organism count, patient compliance and cost of treatment were done. Results: The enrolled patients were suffering from lower urinary tract infections;asymptomatic bacteruria (17 cases) or cystitis (83 cases). Ten patients were excluded. The demographic data of included patients were not significant for both groups. Complete relief (100%) of symptoms 5 days after start of treatment was noticed in Fosfomycin group while improvement of symptoms after 5 day-treatment was noticed in 86.49% in Nitofurantoin group (p-value = 0.030). The side effects were recorded in 7 cases (18.42%) in Fosfomycin group compared to (35.14%) with significant difference展开更多
Objective:To explore the prevention of rerurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa(H.sabdariffa) extract and the biofilm forming capacity of Candida...Objective:To explore the prevention of rerurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa(H.sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H.sabdariffa extract.Methods:In this particular study,six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used.The susceptibility of fungal isolates,time-kill curves and biofilm forming capacity in the present of the H.sabdariffa extract were determined.Results:Various levels minimum inhibitory concentration of the extract were observed against all the isolates.Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL Timekill experiment demonstrated that the effect was fungistatic.The biofilm inhibition assay results showed that H.sabdariffa extract inhibited biofilm production of all the isolates.Conclusions:The results of the study support the potential effect of H.sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.展开更多
The objective of this study was to realize the degree of biosafety precautions and measures recorded among different Private Hospitals and Diagnostic labs personnel situated in different areas of Karachi,Pakistan.One ...The objective of this study was to realize the degree of biosafety precautions and measures recorded among different Private Hospitals and Diagnostic labs personnel situated in different areas of Karachi,Pakistan.One thirty two private hospital based laboratory technicians were selected from all 12 selected areas in Karachi.The laboratory technicians were interviewed then a survey form was completed by the interviewer.The survey was conducted in 3 months duration from May 2017 to July 2017.The total number of members in this study was 132,of which 85 were males and 47 were females.Results presented that 65%of the laboratory technicians did not use any type of PPE and 35%of the respondents recapped used syringes frequently while 25%recapped occasionally.For avoiding the reuse of syringes,Pakistani governments recommend that they be cut before discarding;however,only 65%of the respondents followed the given procedure.Even though mouth pipetting is considered outdated,45%of the technicians continue to do so for numerous purposes.Results of this survey established an absence of awareness about good and proper lab practices and lab biosafety measures among lab technicians in Karachi,Pakistan.展开更多
Introduction:Pakistan Biological Safety Association(PBSA)is a not for profit,nongovernmental,professional organization established in 2008 and is striving to promote and implement Bio risk management in Pakistan.PBSA ...Introduction:Pakistan Biological Safety Association(PBSA)is a not for profit,nongovernmental,professional organization established in 2008 and is striving to promote and implement Bio risk management in Pakistan.PBSA accelerated its program for strategic training of the national life scientists from 2013 and is still working on that mandate with the help of Fogarty International Center(FIC)&National Institute of Health USA and other international organizations working on this Global Health Security Initiative and subsequently Agenda(GHSA)Aims&Objectives:This biosafety training program was developed for capacity building of M.Phil.and PhD graduates the future laboratory users of Basic Medical Sciences Institute(BMSI),Jinnah Post Graduate Medical Center which is the largest tertiary care hospital in Karachi,Pakistan involved in Research&Development(R&D)in medical sciences.Materials&Methods:This Pre post study was carried at Basic Medical Sciences Institute(JPMC),during the period of October 2018 to January 2019.A total of 48(16 PhD.32 M.Phil.)Grads students participated in the following study.The training program is administered through,custom made 4-month course having 16 contact hours also 4 hours of practical training.Training was evaluated using written assessment.The pre and post-training test was conducted after each session.Each test comprised of 15 multiple choice questions(MCQs)pre and post session making it 30 MCQs in total for each session.A final assessment consisting 30 MCQs conducted at the end of course.Results:There was vast difference observed in the post assessment results as compared to the pre assessment results.At the end of the course final assessment was carried out which gave an overall impression of vast change(83.3%)in awareness regarding bio risk management among the graduate students,which reflected the efficacy of this training.To ensure the validity of results and the intention to improvise the quality of course in future,feedback regarding the course and trainer was obtain through Lik展开更多
Over the past 20 years,and with greater urgency since the H5N1 papers controversy(2012),dual-use research of concern(DURC)has been extensively debated by the scientific community,regulators,and the public at large.Man...Over the past 20 years,and with greater urgency since the H5N1 papers controversy(2012),dual-use research of concern(DURC)has been extensively debated by the scientific community,regulators,and the public at large.Many have raised the need to regulate DURC;however,despite the urgency of this issue,to date,only two countries have implemented specific regulations,United States and Israel(notably,China is in the final stages of creating a set of DURC-related regulations).In this paper,I have compared these policies as case studies that may benefit other countries.Therefore,the present study aimed to critically compare the DURC policies of the US and Israel and determine whether their different approaches provide a rigorous framework for the regulation of DURC.I have shown that in some aspects,they have adopted similar approaches;for example,allowing local committees to play a role in assessing the research.However,they differ in certain important aspects;for instance,the US policies have laid out a mechanism through which academic institutions and the government decide jointly on how to address risky studies,while the Israeli law places the authority solely in government hands.My aim in comparing the policies is twofold;first,to reveal the weaknesses and strengths of each policy,thereby aiding their improvement,and second,to provide those that are considering developing policies in this field an overview of what the current approaches look like,thereby enabling them to articulate their own approach while taking into account the experience gained by the US and Israel.展开更多
The Lancet《柳叶刀》英国NJCE发布最新UTIs感控指南 防控泌尿道感染（UTIs）是院感的重要课题，女性住院患者的感染率高达10％～20％。日前，英国NICE发布最新防UTIs抗生素使用指南并指出，虽然在大多数情况下抗生素是必需的，但不适...The Lancet《柳叶刀》英国NJCE发布最新UTIs感控指南 防控泌尿道感染（UTIs）是院感的重要课题，女性住院患者的感染率高达10％～20％。日前，英国NICE发布最新防UTIs抗生素使用指南并指出，虽然在大多数情况下抗生素是必需的，但不适当的治疗会助长日益严重的耐药问题。展开更多
The United Nations Secretary-General’s Mechanism(UNSGM)for investigating the alleged use of biological weapons was proposed in early 2013,with support from the World Organization for Animal Health(OIE)and World Healt...The United Nations Secretary-General’s Mechanism(UNSGM)for investigating the alleged use of biological weapons was proposed in early 2013,with support from the World Organization for Animal Health(OIE)and World Health Organization(WHO).1 The UNSGM and related documents call for designated laboratories to support an investigation of the alleged use of biological weapons.Designed laboratories participating in the UNSGM investigations must not report false results,positive or negative.It is not affordable for whatever reason.展开更多
文摘Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial infections world widely and associated with important medical and financial implications. This retrospective study aimed to establish the bacterial profile of UTIs in Benin. Therefore, reported data were collected from 2003 to 2012 at Menontin’s Hospital in Cotonou and subjected to analyses. At the end of the study, fourteen different bacteria species were found to be associated with UTI cases in Benin. Escherichia coli was the most prevalent followed by Staphylococcus aureus. Results of the current study are helpful to the health care community of Benin in establishing the appropriate antibiotherapy for UTI patients’ treatment with respect organisms into circulation.
文摘Objectives: To evaluate the efficacy, compliance, safety and economic cost for Fosfomycin trometamol and Nitofurantoin in uncomplicated lower urinary tract infections during pregnancy. Background: Nitofurantoin and Fosfomycin trometamol are recommended as the first-line agents for treatment of urinary tract infections (UTIs) in the latest guidelines endorsed by the Infectious Diseases Society of America (IDSA) and the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). Fosfomycin is bactericidal and inhibits bacterial cell wall biogenesis and reduces bacterial adherence to uroepithelial cells. Fosfomycin has broad antibacterial activity against both Gram-positive and Gram-negative pathogens, as Escherichia coli, Escherichia faecalis, and various Gram-negatives like Citrobacter and Proteus. Both Nitofurantoin and Fosfomycin are category B in pregnancy. Patients and Methods: This study was conducted at Tanta University Hospitals in the period from June, 1, 2015 to January, 1, 2017. Patients were recruited from outpatient clinics of Obstetrics and Gynecology and Urology Departments presenting with asymptomatic bacteruria or cystitis. Patients were allocated randomly into 2 groups: group I (n = 50 cases) received Fosfomycin therapy and group II (n = 50 cases) received Nitofurantoin therapy (n = 50 cases). After treatment, evaluation of patient symptoms, organism count, patient compliance and cost of treatment were done. Results: The enrolled patients were suffering from lower urinary tract infections;asymptomatic bacteruria (17 cases) or cystitis (83 cases). Ten patients were excluded. The demographic data of included patients were not significant for both groups. Complete relief (100%) of symptoms 5 days after start of treatment was noticed in Fosfomycin group while improvement of symptoms after 5 day-treatment was noticed in 86.49% in Nitofurantoin group (p-value = 0.030). The side effects were recorded in 7 cases (18.42%) in Fosfomycin group compared to (35.14%) with significant difference
基金Supported by the Deanship of Scientific Research of Taibah University (Grant No.432/3088)
文摘Objective:To explore the prevention of rerurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa(H.sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H.sabdariffa extract.Methods:In this particular study,six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used.The susceptibility of fungal isolates,time-kill curves and biofilm forming capacity in the present of the H.sabdariffa extract were determined.Results:Various levels minimum inhibitory concentration of the extract were observed against all the isolates.Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL Timekill experiment demonstrated that the effect was fungistatic.The biofilm inhibition assay results showed that H.sabdariffa extract inhibited biofilm production of all the isolates.Conclusions:The results of the study support the potential effect of H.sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.
文摘The objective of this study was to realize the degree of biosafety precautions and measures recorded among different Private Hospitals and Diagnostic labs personnel situated in different areas of Karachi,Pakistan.One thirty two private hospital based laboratory technicians were selected from all 12 selected areas in Karachi.The laboratory technicians were interviewed then a survey form was completed by the interviewer.The survey was conducted in 3 months duration from May 2017 to July 2017.The total number of members in this study was 132,of which 85 were males and 47 were females.Results presented that 65%of the laboratory technicians did not use any type of PPE and 35%of the respondents recapped used syringes frequently while 25%recapped occasionally.For avoiding the reuse of syringes,Pakistani governments recommend that they be cut before discarding;however,only 65%of the respondents followed the given procedure.Even though mouth pipetting is considered outdated,45%of the technicians continue to do so for numerous purposes.Results of this survey established an absence of awareness about good and proper lab practices and lab biosafety measures among lab technicians in Karachi,Pakistan.
文摘Introduction:Pakistan Biological Safety Association(PBSA)is a not for profit,nongovernmental,professional organization established in 2008 and is striving to promote and implement Bio risk management in Pakistan.PBSA accelerated its program for strategic training of the national life scientists from 2013 and is still working on that mandate with the help of Fogarty International Center(FIC)&National Institute of Health USA and other international organizations working on this Global Health Security Initiative and subsequently Agenda(GHSA)Aims&Objectives:This biosafety training program was developed for capacity building of M.Phil.and PhD graduates the future laboratory users of Basic Medical Sciences Institute(BMSI),Jinnah Post Graduate Medical Center which is the largest tertiary care hospital in Karachi,Pakistan involved in Research&Development(R&D)in medical sciences.Materials&Methods:This Pre post study was carried at Basic Medical Sciences Institute(JPMC),during the period of October 2018 to January 2019.A total of 48(16 PhD.32 M.Phil.)Grads students participated in the following study.The training program is administered through,custom made 4-month course having 16 contact hours also 4 hours of practical training.Training was evaluated using written assessment.The pre and post-training test was conducted after each session.Each test comprised of 15 multiple choice questions(MCQs)pre and post session making it 30 MCQs in total for each session.A final assessment consisting 30 MCQs conducted at the end of course.Results:There was vast difference observed in the post assessment results as compared to the pre assessment results.At the end of the course final assessment was carried out which gave an overall impression of vast change(83.3%)in awareness regarding bio risk management among the graduate students,which reflected the efficacy of this training.To ensure the validity of results and the intention to improvise the quality of course in future,feedback regarding the course and trainer was obtain through Lik
基金Financial support for this research was provided by The Israeli Ministry of Science,Technology,and Space(Grant no.3-10739).
文摘Over the past 20 years,and with greater urgency since the H5N1 papers controversy(2012),dual-use research of concern(DURC)has been extensively debated by the scientific community,regulators,and the public at large.Many have raised the need to regulate DURC;however,despite the urgency of this issue,to date,only two countries have implemented specific regulations,United States and Israel(notably,China is in the final stages of creating a set of DURC-related regulations).In this paper,I have compared these policies as case studies that may benefit other countries.Therefore,the present study aimed to critically compare the DURC policies of the US and Israel and determine whether their different approaches provide a rigorous framework for the regulation of DURC.I have shown that in some aspects,they have adopted similar approaches;for example,allowing local committees to play a role in assessing the research.However,they differ in certain important aspects;for instance,the US policies have laid out a mechanism through which academic institutions and the government decide jointly on how to address risky studies,while the Israeli law places the authority solely in government hands.My aim in comparing the policies is twofold;first,to reveal the weaknesses and strengths of each policy,thereby aiding their improvement,and second,to provide those that are considering developing policies in this field an overview of what the current approaches look like,thereby enabling them to articulate their own approach while taking into account the experience gained by the US and Israel.
文摘The United Nations Secretary-General’s Mechanism(UNSGM)for investigating the alleged use of biological weapons was proposed in early 2013,with support from the World Organization for Animal Health(OIE)and World Health Organization(WHO).1 The UNSGM and related documents call for designated laboratories to support an investigation of the alleged use of biological weapons.Designed laboratories participating in the UNSGM investigations must not report false results,positive or negative.It is not affordable for whatever reason.
