目的:探究不同强度一次性运动干预对大鼠睾周白色脂肪中FNDC5和UCP-1表达量的影响。方法:30只SD大鼠随机分为对照组(C组,n=6)和运动组(E组,n=24)。E组大鼠随机分为中等强度运动组(EM组,n=12,15 m/min)和高强度间歇运动组(EH组,n=12,35 m...目的:探究不同强度一次性运动干预对大鼠睾周白色脂肪中FNDC5和UCP-1表达量的影响。方法:30只SD大鼠随机分为对照组(C组,n=6)和运动组(E组,n=24)。E组大鼠随机分为中等强度运动组(EM组,n=12,15 m/min)和高强度间歇运动组(EH组,n=12,35 m/min,6 min间歇5 min,重复3次),分别在一次性运动后即刻和6 h后取大鼠睾周白色脂肪,利用RT-q PCR法检测睾周白色脂肪组织中细胞外跨膜受体Ⅲ型纤连蛋白域蛋白5(FNDC5)和解偶联蛋白1(UCP-1)m RNA表达水平,Western Blot法检测睾周白色脂肪组织中FNDC5和UCP-1蛋白含量。结果:(1)与C组相比,EH组运动后即刻FNDC5 m RNA表达水平显著升高(P<0.01)。(2)与C组相比,运动组大鼠睾周白色脂肪组织中UCP-1m RNA水平在运动后即刻显著降低(P<0.05);与运动后即刻相比较,运动后6 h显著升高(P<0.05)。(3)与C组相比,EM和EH组FNDC5蛋白含量均有上升趋势,其中EM组显著升高(P<0.05)。(4)与C组相比,EM组和EH组UCP-1蛋白含量均有上升趋势,其中EH组运动后即刻出现显著升高(P<0.05)。结论:(1)一次性运动能够提高大鼠睾周白色脂肪FNDC5和UCP-1蛋白表达水平,促进白色脂肪棕色化,增加脂肪组织产热;(2)一次性运动后FNDC5 m RNA和UCP-1 m RNA的变化具有时效性,其中UCP-1 m RNA在运动后6小时出现显著升高,而FNDC5 m RNA在高强度间歇运动后即刻变化最明显。展开更多
Objective： To explore the effect of peroxisome proliferators-activated receptor γ coactivator-1α（PGC-1α） and uncoupling protein-1（UCP-1） in the brown adipose tissue（BAT） of obese rats in the process of acupu...Objective： To explore the effect of peroxisome proliferators-activated receptor γ coactivator-1α（PGC-1α） and uncoupling protein-1（UCP-1） in the brown adipose tissue（BAT） of obese rats in the process of acupuncture treatment for obesity.Methods： Fifty clean-grade male Wistar rats with the age of 3 months were randomly divided into highfat diet group（n = 40） and normal diet group（control group, n = 10）. Nutritional obesity animal models were established through feeding with high-fat diet. Twenty-four animal models in the high-fat diet group were established successfully, and then they were randomly divided into model group, acupuncture group and non-acupoint group,with 8 rats in each group. In acupuncture group, Zusanli（足三里ST36） and Tianshu（天枢ST 25） were selected. In non-acupoint group,the non-acupoints located on 5 mm beside ST 36 and ST 25 were selected as acupuncture points, and electroacupuncture intervention was adopted for 5 times/week for 8 weeks. The body mass of obese rats was measured, the body fat ratio in BAT of rats was calculated, and the expression levels of PGC-1α and UCP-1 in BAT of rats were detected by immunohistochemical method.Results： ① After treatment, the body mass of rats in acupuncture group reduced significantly, which increased in the other three groups. The changing value of body mass of rats in acupuncture group was higher than that in model group（P 0.05）, the changing value of body mass of rats in acupuncture group was higher than that in non-acupoint group（P 0.05） and the difference in body mass changing value of rats between non-acupoint group and model group was not statistically significant（P 0.05）,the body fat ratio in BAT of rats in non-acupoint group was lower than that in acupuncture group, and the differences were statistically significant（P0.05）. ② Compared with model group, the body fat ratio in BAT of rats in acupuncture group increased significantly（P 0.05）,and the difference in body fat ratio in BAT 展开更多
文摘目的:探究不同强度一次性运动干预对大鼠睾周白色脂肪中FNDC5和UCP-1表达量的影响。方法:30只SD大鼠随机分为对照组(C组,n=6)和运动组(E组,n=24)。E组大鼠随机分为中等强度运动组(EM组,n=12,15 m/min)和高强度间歇运动组(EH组,n=12,35 m/min,6 min间歇5 min,重复3次),分别在一次性运动后即刻和6 h后取大鼠睾周白色脂肪,利用RT-q PCR法检测睾周白色脂肪组织中细胞外跨膜受体Ⅲ型纤连蛋白域蛋白5(FNDC5)和解偶联蛋白1(UCP-1)m RNA表达水平,Western Blot法检测睾周白色脂肪组织中FNDC5和UCP-1蛋白含量。结果:(1)与C组相比,EH组运动后即刻FNDC5 m RNA表达水平显著升高(P<0.01)。(2)与C组相比,运动组大鼠睾周白色脂肪组织中UCP-1m RNA水平在运动后即刻显著降低(P<0.05);与运动后即刻相比较,运动后6 h显著升高(P<0.05)。(3)与C组相比,EM和EH组FNDC5蛋白含量均有上升趋势,其中EM组显著升高(P<0.05)。(4)与C组相比,EM组和EH组UCP-1蛋白含量均有上升趋势,其中EH组运动后即刻出现显著升高(P<0.05)。结论:(1)一次性运动能够提高大鼠睾周白色脂肪FNDC5和UCP-1蛋白表达水平,促进白色脂肪棕色化,增加脂肪组织产热;(2)一次性运动后FNDC5 m RNA和UCP-1 m RNA的变化具有时效性,其中UCP-1 m RNA在运动后6小时出现显著升高,而FNDC5 m RNA在高强度间歇运动后即刻变化最明显。
基金Supported by National Natural Science Foundation of China:81674081~~
文摘Objective： To explore the effect of peroxisome proliferators-activated receptor γ coactivator-1α（PGC-1α） and uncoupling protein-1（UCP-1） in the brown adipose tissue（BAT） of obese rats in the process of acupuncture treatment for obesity.Methods： Fifty clean-grade male Wistar rats with the age of 3 months were randomly divided into highfat diet group（n = 40） and normal diet group（control group, n = 10）. Nutritional obesity animal models were established through feeding with high-fat diet. Twenty-four animal models in the high-fat diet group were established successfully, and then they were randomly divided into model group, acupuncture group and non-acupoint group,with 8 rats in each group. In acupuncture group, Zusanli（足三里ST36） and Tianshu（天枢ST 25） were selected. In non-acupoint group,the non-acupoints located on 5 mm beside ST 36 and ST 25 were selected as acupuncture points, and electroacupuncture intervention was adopted for 5 times/week for 8 weeks. The body mass of obese rats was measured, the body fat ratio in BAT of rats was calculated, and the expression levels of PGC-1α and UCP-1 in BAT of rats were detected by immunohistochemical method.Results： ① After treatment, the body mass of rats in acupuncture group reduced significantly, which increased in the other three groups. The changing value of body mass of rats in acupuncture group was higher than that in model group（P 0.05）, the changing value of body mass of rats in acupuncture group was higher than that in non-acupoint group（P 0.05） and the difference in body mass changing value of rats between non-acupoint group and model group was not statistically significant（P 0.05）,the body fat ratio in BAT of rats in non-acupoint group was lower than that in acupuncture group, and the differences were statistically significant（P0.05）. ② Compared with model group, the body fat ratio in BAT of rats in acupuncture group increased significantly（P 0.05）,and the difference in body fat ratio in BAT
