Glial cells play an important role in signal transduction,energy metabolism,extracellular ion homeostasis and neuroprotection of the central nervous system.However,few studies have explained the potential effects of e...Glial cells play an important role in signal transduction,energy metabolism,extracellular ion homeostasis and neuroprotection of the central nervous system.However,few studies have explained the potential effects of exosomes from glial cells on central nervous system health and disease.In this study,the genes expressed in exosomes from astrocytes and microglia were identified by deep RNA sequencing.Kyoto Encyclopedia of Genes and Genomes analysis indicated that several pathways in these exosomes are responsible for promoting neurodegenerative diseases,including Alzheimer's disease,Parkinson's disease and Huntington's disease.Gene ontology analysis showed that extracellular exosome,mitochondrion and growth factor activity were enriched in exosomes from the unique astrocyte group,while extracellular exosome and mitochondrion were enriched in exosomes from the unique microglia group.Next,combined with the screening of hub genes,the protein-protein interaction network analysis showed that exosomes from astrocytes influence neurodegenerative diseases through metabolic balance and ubiquitin-dependent protein balance,whereas exosomes from microglia influence neurodegenerative diseases through immune inflammation and oxidative stress.Although there were differences in RNA expression between exosomes from astrocytes and microglia,the groups were related by the hub genes,ubiquitin B and heat shock protein family A(Hsp70) member 8.Ubiquitin B appeared to be involved in pleiotropic regulatory functions,including immune regulation,inflammation inhibition,protein catabolism,intracellular protein transport,exosomes and oxidative stress.The results revealed the clinical significance of exosomes from glia in neurodegenerative diseases.This study was approved by the Animal Ethics Committee of Nantong University,China(approval No.S20180102-152) on January 2,2018.展开更多
The vertex solution for estimation on the static displacement bounds of structures with uncertain-but-bounded parameters is studied in this paper. For the linear static problem, when there are uncertain interval param...The vertex solution for estimation on the static displacement bounds of structures with uncertain-but-bounded parameters is studied in this paper. For the linear static problem, when there are uncertain interval parameters in the stiffness matrix and the vector of applied forces, the static response may be an interval. Based on the interval operations, the interval solution obtained by the vertex solution is more accurate and more credible than other methods （such as the perturbation method）. However, the vertex solution method by traditional serial computing usually needs large computational efforts, especially for large structures. In order to avoid its disadvantages of large calculation and much runtime, its parallel computing which can be used in large-scale computing is presented in this paper. Two kinds of parallel computing algorithms are proposed based on the vertex solution. The parallel computing will solve many interval problems which cannot be resolved by traditional interval analysis methods.展开更多
2010年4月5日15时2分左右,美国西弗吉尼亚州罗利县,梅西能源公司(Massey Energy)的上大分支地下煤矿(Upper Big Branch,UBB)发生瓦斯爆炸,造成29名矿工死亡,2人受伤,成为美国近40多年伤亡最严重的矿难事故,引发对矿山安全的大规模关注...2010年4月5日15时2分左右,美国西弗吉尼亚州罗利县,梅西能源公司(Massey Energy)的上大分支地下煤矿(Upper Big Branch,UBB)发生瓦斯爆炸,造成29名矿工死亡,2人受伤,成为美国近40多年伤亡最严重的矿难事故,引发对矿山安全的大规模关注。而梅西能源公司时任CEO也因故意违反矿山安全健康相关法律被法院判处监禁12个月。展开更多
面向千兆宽带、无处不在的视频、智慧家庭和室内物联网、随需网络（Network On Demand）四大核心超宽带业务,华为在2015年7月面向全球发布了UBB 2020战略全景。公开信息显示,全球超过20家运营商已运营智慧家庭业务及相关服务,超过50家...面向千兆宽带、无处不在的视频、智慧家庭和室内物联网、随需网络（Network On Demand）四大核心超宽带业务,华为在2015年7月面向全球发布了UBB 2020战略全景。公开信息显示,全球超过20家运营商已运营智慧家庭业务及相关服务,超过50家运营商正提供千兆宽带业务,超过60家运营商将视频作为基础业务,其中大部分运营商正计划发布超高清视频（4K）业务,而超过100家以移动业务为主的运营商已宣布将大幅增加对固定宽带业务和网络的投资,超宽带产业正进入一个过去20多年从未有过的＂最好时期＂。展开更多
基金supported by the National Natural Science Foundation of China,No.81870975 (to SLZ)Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province of China,No.202010304034Z (to FYZ)。
文摘Glial cells play an important role in signal transduction,energy metabolism,extracellular ion homeostasis and neuroprotection of the central nervous system.However,few studies have explained the potential effects of exosomes from glial cells on central nervous system health and disease.In this study,the genes expressed in exosomes from astrocytes and microglia were identified by deep RNA sequencing.Kyoto Encyclopedia of Genes and Genomes analysis indicated that several pathways in these exosomes are responsible for promoting neurodegenerative diseases,including Alzheimer's disease,Parkinson's disease and Huntington's disease.Gene ontology analysis showed that extracellular exosome,mitochondrion and growth factor activity were enriched in exosomes from the unique astrocyte group,while extracellular exosome and mitochondrion were enriched in exosomes from the unique microglia group.Next,combined with the screening of hub genes,the protein-protein interaction network analysis showed that exosomes from astrocytes influence neurodegenerative diseases through metabolic balance and ubiquitin-dependent protein balance,whereas exosomes from microglia influence neurodegenerative diseases through immune inflammation and oxidative stress.Although there were differences in RNA expression between exosomes from astrocytes and microglia,the groups were related by the hub genes,ubiquitin B and heat shock protein family A(Hsp70) member 8.Ubiquitin B appeared to be involved in pleiotropic regulatory functions,including immune regulation,inflammation inhibition,protein catabolism,intracellular protein transport,exosomes and oxidative stress.The results revealed the clinical significance of exosomes from glia in neurodegenerative diseases.This study was approved by the Animal Ethics Committee of Nantong University,China(approval No.S20180102-152) on January 2,2018.
基金supported by the National Outstanding Youth Science Foundation of China (No.10425208)111 Project(No.B07009)FanZhou Science and Research Foundation for Young Scholars (No.20080503).
文摘The vertex solution for estimation on the static displacement bounds of structures with uncertain-but-bounded parameters is studied in this paper. For the linear static problem, when there are uncertain interval parameters in the stiffness matrix and the vector of applied forces, the static response may be an interval. Based on the interval operations, the interval solution obtained by the vertex solution is more accurate and more credible than other methods （such as the perturbation method）. However, the vertex solution method by traditional serial computing usually needs large computational efforts, especially for large structures. In order to avoid its disadvantages of large calculation and much runtime, its parallel computing which can be used in large-scale computing is presented in this paper. Two kinds of parallel computing algorithms are proposed based on the vertex solution. The parallel computing will solve many interval problems which cannot be resolved by traditional interval analysis methods.
文摘2010年4月5日15时2分左右,美国西弗吉尼亚州罗利县,梅西能源公司(Massey Energy)的上大分支地下煤矿(Upper Big Branch,UBB)发生瓦斯爆炸,造成29名矿工死亡,2人受伤,成为美国近40多年伤亡最严重的矿难事故,引发对矿山安全的大规模关注。而梅西能源公司时任CEO也因故意违反矿山安全健康相关法律被法院判处监禁12个月。
文摘面向千兆宽带、无处不在的视频、智慧家庭和室内物联网、随需网络（Network On Demand）四大核心超宽带业务,华为在2015年7月面向全球发布了UBB 2020战略全景。公开信息显示,全球超过20家运营商已运营智慧家庭业务及相关服务,超过50家运营商正提供千兆宽带业务,超过60家运营商将视频作为基础业务,其中大部分运营商正计划发布超高清视频（4K）业务,而超过100家以移动业务为主的运营商已宣布将大幅增加对固定宽带业务和网络的投资,超宽带产业正进入一个过去20多年从未有过的＂最好时期＂。
