Mixed-model U-shaped assembly line balancing problems (MMUALBP) is known to be NP-hard resulting in it being nearly impossible to obtain an optimal solution for practical problems with deterministic algorithms. This p...Mixed-model U-shaped assembly line balancing problems (MMUALBP) is known to be NP-hard resulting in it being nearly impossible to obtain an optimal solution for practical problems with deterministic algorithms. This paper pre-sents a new evolutionary method called combinatorial optimisation with coincidence algorithm (COIN) being applied to Type I problems of MMUALBP in a just-in-time production system. Three objectives are simultaneously considered;minimum number workstations, minimum work relatedness, and minimum workload smoothness. The variances of COIN are also proposed, i.e. CNSGA II, and COIN-MA. COIN and its variances are tested against a well-known algo-rithm namely non-dominated sorting genetic algorithm II (NSGA II) and MNSGA II (a memetic version of NSGA II). Experimental results showed that COIN outperformed NSGA II. In addition, although COIN-MA uses a marginal CPU time than CNSGA II, its other performances are dominated.展开更多
目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保...目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保藏中心(Deutsche Sammlung von Mikroorganismen und Zellkulturen,DSMZ)数据库中U-87 MG]进行细胞生物学特性测定,对两株细胞用于后续研究工作的可能性进行评价。方法分别采用CCK-8法、划痕实验、Transwell实验等方法,并结合显微镜下细胞形态学特征,比较两株肿瘤细胞系的增生、迁移及侵袭能力等生物学特性。结果 (1)CCK-8法:细胞生长曲线提示细胞增生能力差异无统计学意义(P>0.05)。(2)划痕实验:两细胞系迁移能力差异无统计学意义(P>0.05)。(3)Transwell实验:两细胞系迁移能力差异无统计学意义(P>0.05),DSMZ数据库中U-87 MG细胞侵袭能力高于ATCC数据库中U-87 MG GlioblastomaAstrocytoma Human细胞,差异有统计学意义(P<0.05)。结论两株U87细胞系均可反映脑胶质瘤的生物学特性,可用于后续科研实验。两细胞系的增生、迁移能力差异无统计学意义,侵袭能力差异具有统计学意义,可根据实验目的选择合适的细胞系。展开更多
文摘Mixed-model U-shaped assembly line balancing problems (MMUALBP) is known to be NP-hard resulting in it being nearly impossible to obtain an optimal solution for practical problems with deterministic algorithms. This paper pre-sents a new evolutionary method called combinatorial optimisation with coincidence algorithm (COIN) being applied to Type I problems of MMUALBP in a just-in-time production system. Three objectives are simultaneously considered;minimum number workstations, minimum work relatedness, and minimum workload smoothness. The variances of COIN are also proposed, i.e. CNSGA II, and COIN-MA. COIN and its variances are tested against a well-known algo-rithm namely non-dominated sorting genetic algorithm II (NSGA II) and MNSGA II (a memetic version of NSGA II). Experimental results showed that COIN outperformed NSGA II. In addition, although COIN-MA uses a marginal CPU time than CNSGA II, its other performances are dominated.
文摘目的对经过短串联重复序列(short tandem repeats,STR)分型检测技术鉴定后的两株U87胶质瘤细胞系[(美国典型培养物保藏中心(American Type Culture Collection,ATCC)数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保藏中心(Deutsche Sammlung von Mikroorganismen und Zellkulturen,DSMZ)数据库中U-87 MG]进行细胞生物学特性测定,对两株细胞用于后续研究工作的可能性进行评价。方法分别采用CCK-8法、划痕实验、Transwell实验等方法,并结合显微镜下细胞形态学特征,比较两株肿瘤细胞系的增生、迁移及侵袭能力等生物学特性。结果 (1)CCK-8法:细胞生长曲线提示细胞增生能力差异无统计学意义(P>0.05)。(2)划痕实验:两细胞系迁移能力差异无统计学意义(P>0.05)。(3)Transwell实验:两细胞系迁移能力差异无统计学意义(P>0.05),DSMZ数据库中U-87 MG细胞侵袭能力高于ATCC数据库中U-87 MG GlioblastomaAstrocytoma Human细胞,差异有统计学意义(P<0.05)。结论两株U87细胞系均可反映脑胶质瘤的生物学特性,可用于后续科研实验。两细胞系的增生、迁移能力差异无统计学意义,侵袭能力差异具有统计学意义,可根据实验目的选择合适的细胞系。
