In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch（THMS） and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, wh...In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch（THMS） and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, which consists of a central section with a shortened 8-mer aptamer sequence with high affinity to tetracycline and flanked by two arm segments. G-rich oligonucleotide can specifically bind to thioflavin T（Th T） as a signal transduction probe（STP）. In the absence of tetracycline, THMS remains stable, the fluorescence of background is low. By the addition of target tetracycline, the aptamer-target binding results in the formation of a structured aptamer-target complex, which disassembles the THMS and releases the STP. The free STP self-assembles into G-quadruplex and specifically binds to Th T which generates a obvious fluorescence enhancement. Using the triple-helix molecular switch, the developed aptamer-based fluorescent sensing platform showed a linear relationship with the concentration of tetracycline ranging from 0.2 to 20.0 nmol/L. The detection limit of tetracycline was determined to be970.0 pmol/L. The assay avoids complicated modifications or chemical labeling, making it simple and cost-effective. So, it is expected that this aptamer-based fluorescent assay could be extensively applied in the field of food safety inspection.展开更多
This paper goes beyond Sino-Nordic Arctic science diplomacy and looks at Sino-Nordic Arctic triple-helix knowledge- based collaborations among academia, business, civil society （the inclusion of which moves beyond th...This paper goes beyond Sino-Nordic Arctic science diplomacy and looks at Sino-Nordic Arctic triple-helix knowledge- based collaborations among academia, business, civil society （the inclusion of which moves beyond the original triple-helix concept）, and government. In light of the potential of science diplomacy for building Sino-Arctic trust under systemic international conditions of power transition and globalization, this is the natural next step toward exploring triple-helix collaborations. Knowledge-based collaborations between academia, business, civil society, and government also open up avenues for innovation and entrepreneurship by both Arctic societies and Chinese society in attempts to address major challenges to sustainable development in these societies. This paper discusses possible triple-helix knowledge-based collaborations with China by each of the five Nordic countries, and highlights the innovation and entrepreneurial talents of summer school students at the University of International Relations in Beijing in developing projects as part of a course entitled ＂The Global Arctic＂.展开更多
基金supported by National Natural Science Foundation of China (Nos. 21205142, 31370104)The Research Innovation Program for Graduates of Central South University (No. 2016zzts580)
文摘In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch（THMS） and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, which consists of a central section with a shortened 8-mer aptamer sequence with high affinity to tetracycline and flanked by two arm segments. G-rich oligonucleotide can specifically bind to thioflavin T（Th T） as a signal transduction probe（STP）. In the absence of tetracycline, THMS remains stable, the fluorescence of background is low. By the addition of target tetracycline, the aptamer-target binding results in the formation of a structured aptamer-target complex, which disassembles the THMS and releases the STP. The free STP self-assembles into G-quadruplex and specifically binds to Th T which generates a obvious fluorescence enhancement. Using the triple-helix molecular switch, the developed aptamer-based fluorescent sensing platform showed a linear relationship with the concentration of tetracycline ranging from 0.2 to 20.0 nmol/L. The detection limit of tetracycline was determined to be970.0 pmol/L. The assay avoids complicated modifications or chemical labeling, making it simple and cost-effective. So, it is expected that this aptamer-based fluorescent assay could be extensively applied in the field of food safety inspection.
基金China Nordic Arctic Research Center(Polar Research Institute of China)for providing me with a visiting fellowship from 1 March to 28 April,2016funding from the Department of Sociology,Political Science and Community Planning,and the HSL-utdanningsfond(Faculty of Humanities,Social Sciences and Education Teaching Fund),University of Tromso-The Arctic University of Norway,to attend the China-Nordic Arctic Cooperation Symposium in Rovaniemi from 6 to 9 June,2016
文摘This paper goes beyond Sino-Nordic Arctic science diplomacy and looks at Sino-Nordic Arctic triple-helix knowledge- based collaborations among academia, business, civil society （the inclusion of which moves beyond the original triple-helix concept）, and government. In light of the potential of science diplomacy for building Sino-Arctic trust under systemic international conditions of power transition and globalization, this is the natural next step toward exploring triple-helix collaborations. Knowledge-based collaborations between academia, business, civil society, and government also open up avenues for innovation and entrepreneurship by both Arctic societies and Chinese society in attempts to address major challenges to sustainable development in these societies. This paper discusses possible triple-helix knowledge-based collaborations with China by each of the five Nordic countries, and highlights the innovation and entrepreneurial talents of summer school students at the University of International Relations in Beijing in developing projects as part of a course entitled ＂The Global Arctic＂.
文摘"政府—产业—大学"(官产学)三螺旋创新范式自从在1995年被亨利·埃茨科威兹(Henry Etzkowitz)和劳埃特·雷德斯多夫(Loet Leydesdorff)首次提出以来,尚未发现有相关研究对官产学三螺旋创新国际研究成果展开全面、系统的梳理与分析。鉴于此,以Web of Science(WoS)核心合集的SCI-EXPANDED、SSCI两大数据库所收录的1995—2019年期间三螺旋研究文献及其参考文献为样本,综合使用共被引分析、引文网络关系分析、主路径分析和文献耦合分析等多种基于引文分析的量化方法,对官产学三螺旋创新的国际研究成果及其知识基础展开全方位系统研究。结果发现三螺旋创新核心思想和研究方法受到信息熵理论、演化经济学理论、三元模型理论、"模式2"知识生产模式和国家创新系统理论的影响;三螺旋创新研究已经由早期的基础概念性理论阐述逐渐转向了对创新主体三螺旋非线性互动的量化研究,但是很多研究仅局限于对非线性互动关系的简单描述与刻画;同时还发现三螺旋创新研究领域存在两个派别,分别是基于三螺旋算法的定量研究派别和基于三螺旋框架的定性研究派别。最后,从三螺旋主体间关系、国家情境与研究方法等三个方面提出了未来研究展望。总之,本研究采取一种较为新颖的文献综述方法对三螺旋研究文献展开梳理与分析,为未来综述类文献在研究方法上对某领域展开全面深入的定量研究提供了理论启示,同时为后发国家引入欧美发达国家的三螺旋创新思想来指导本国的创新实践和制定相关政策提供了实践启示。
