目的探讨肺癌术后肺部感染的病原菌特征及对血清髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)、降钙素原(procalcitotin,PCT)、血管内皮细胞生长因子(vascular endothelial growth f...目的探讨肺癌术后肺部感染的病原菌特征及对血清髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)、降钙素原(procalcitotin,PCT)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)水平的影响。方法选取2014年1月至2017年12月金华市中心医院和绍兴市人民医院收治的肺癌术后患者300例,其中发生肺部感染者35例作为感染组,随机选取未发生肺部感染者35例作为非感染组,健康体检者35例作为对照组。检测肺部感染的病原学分布和耐药情况,测定血清sTREM-1、PCT、VEGF水平。结果35例肺部感染患者中痰液培养阳性33例,共培养出菌株42株。革兰阳性菌9株(21.43%),主要为金黄色葡萄球菌6株(14.29%);革兰阴性菌31株(73.81%),主要为肺炎克雷伯菌10株(23.81%)和鲍曼不动杆菌和9株(21.43%);真菌2株(4.76%)。金黄色葡萄球菌对青霉素100.00%耐药,其次为苯唑西林和克林霉素,耐药率分别为66.67%和50.00%。肺炎克雷伯菌对氨苄西林和环丙沙星耐药率高,耐药率分别为90.00%和60.00%;鲍曼不动杆菌主要对氨苄西林、环丙沙星、阿米卡星耐药率高,耐药率分别为77.78%、66.67%和66.67%。感染组血清sTREM-1、PCT、VEGF水平均高于非感染组和对照组,差异有统计学意义(F=227.821、1311.052、25.020,P<0.01)。结论肺癌术后肺部感染的病原菌以革兰阴性菌为主,肺部感染患者血清sTREM-1、PCT、VEGF水平升高。展开更多
新生儿败血症是新生儿期常见的重症感染性疾病,其病情进展迅速,是新生儿期死亡的重要原因。可溶性髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)是髓样细胞触发受体-1(triggering rec...新生儿败血症是新生儿期常见的重症感染性疾病,其病情进展迅速,是新生儿期死亡的重要原因。可溶性髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)是髓样细胞触发受体-1(triggering receptors expressed on myeloid cell-1,TREM-1)的可溶性亚型,在炎症反应中高度表达,与多种细胞受体协同作用可激活细胞通路及放大炎症反应。大量研究证实sTREM-1在新生儿败血症的早期识别、病情进展及预后评估方面具有重要作用。该文对sTREM-1的生物学特性、信号通路及在新生儿败血症中的作用进行综述。展开更多
Background： Triggering receptor expressed on myeloid cell- 1 （TREM- 1） may play a vital role in mammalian target ofrapamycin （mTOR） modulation ofCD8＋ T-cell differentiation through the transcription factors T-bo...Background： Triggering receptor expressed on myeloid cell- 1 （TREM- 1） may play a vital role in mammalian target ofrapamycin （mTOR） modulation ofCD8＋ T-cell differentiation through the transcription factors T-box expressed in T-cells and eomesodermin during the immune response to invasive pulmonary aspergillosis （IPA）. This study aimed to investigate whether the roTOR signaling pathway modulates the proliferation and differentiation of CD8＋ T-cells during the immune response to I PA and the role TREM-1 plays in this process. Methods： Cyclophosphamide （CTX） was injected intraperitoneally, and Asl？e;gillus.[mnigams spore suspension was inoculated intranasally to establish the immunosuppressed IPA mouse model. After inoculation, rapamycin （2 mg-kg ·d -1） or interleukin （IL）-12 （5 μg/kg every other day） was given for 7 days. The number of CD8＋ effector memory T-cells （Tern）, expression of interferon （IFN）-y, roTOR, and ribosomal protein $6 kinase （S6K）, and the levels of IL-6, IL- 10, galactomannan （GM）, and soluble TREM- 1 （sTREM-I） were measured. Results： Viable A. fumigatus was cultured from the lung tissue of the inoculated mice. Histological examination indicated greater inflammation, hemorrhage, and lung tissue injury in both IPA and CTX ＋ IPA mice groups. The expression of mTOR and S6K was significantly increased in the CTX ＋ IPA ＋ I L- 12 group compared with the control, I PA （P = 0.01 ; P - 0.001 ）, and CTX ＋ 1PA （P = 0.034; P = 0.032） groups, but significantly decreased in the CTX ＋ IPA ＋ RAPA group （P 〈 0.001 ）. Compared with the CTX ＋ IPA group, the proportion of Tern, expression of IFN-y, and the level ofsTREM-I were significantly higher after IL-12 treatment （P = 0.024, P = 0.032, and P = 0.017, respectively）, and the opposite results were observed when the roTOR pathway was blocked by rapamycin （P 〈 0.001）. Compared with the CTX ＋ I PA and CTX ＋ I PA ＋ RAPA groups, IL-12 treatment increased IL-6 展开更多
文摘目的探讨肺癌术后肺部感染的病原菌特征及对血清髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)、降钙素原(procalcitotin,PCT)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)水平的影响。方法选取2014年1月至2017年12月金华市中心医院和绍兴市人民医院收治的肺癌术后患者300例,其中发生肺部感染者35例作为感染组,随机选取未发生肺部感染者35例作为非感染组,健康体检者35例作为对照组。检测肺部感染的病原学分布和耐药情况,测定血清sTREM-1、PCT、VEGF水平。结果35例肺部感染患者中痰液培养阳性33例,共培养出菌株42株。革兰阳性菌9株(21.43%),主要为金黄色葡萄球菌6株(14.29%);革兰阴性菌31株(73.81%),主要为肺炎克雷伯菌10株(23.81%)和鲍曼不动杆菌和9株(21.43%);真菌2株(4.76%)。金黄色葡萄球菌对青霉素100.00%耐药,其次为苯唑西林和克林霉素,耐药率分别为66.67%和50.00%。肺炎克雷伯菌对氨苄西林和环丙沙星耐药率高,耐药率分别为90.00%和60.00%;鲍曼不动杆菌主要对氨苄西林、环丙沙星、阿米卡星耐药率高,耐药率分别为77.78%、66.67%和66.67%。感染组血清sTREM-1、PCT、VEGF水平均高于非感染组和对照组,差异有统计学意义(F=227.821、1311.052、25.020,P<0.01)。结论肺癌术后肺部感染的病原菌以革兰阴性菌为主,肺部感染患者血清sTREM-1、PCT、VEGF水平升高。
文摘新生儿败血症是新生儿期常见的重症感染性疾病,其病情进展迅速,是新生儿期死亡的重要原因。可溶性髓样细胞触发受体-1(soluble form of triggering receptors expressed on myeloid cell-1,sTREM-1)是髓样细胞触发受体-1(triggering receptors expressed on myeloid cell-1,TREM-1)的可溶性亚型,在炎症反应中高度表达,与多种细胞受体协同作用可激活细胞通路及放大炎症反应。大量研究证实sTREM-1在新生儿败血症的早期识别、病情进展及预后评估方面具有重要作用。该文对sTREM-1的生物学特性、信号通路及在新生儿败血症中的作用进行综述。
文摘Background： Triggering receptor expressed on myeloid cell- 1 （TREM- 1） may play a vital role in mammalian target ofrapamycin （mTOR） modulation ofCD8＋ T-cell differentiation through the transcription factors T-box expressed in T-cells and eomesodermin during the immune response to invasive pulmonary aspergillosis （IPA）. This study aimed to investigate whether the roTOR signaling pathway modulates the proliferation and differentiation of CD8＋ T-cells during the immune response to I PA and the role TREM-1 plays in this process. Methods： Cyclophosphamide （CTX） was injected intraperitoneally, and Asl？e;gillus.[mnigams spore suspension was inoculated intranasally to establish the immunosuppressed IPA mouse model. After inoculation, rapamycin （2 mg-kg ·d -1） or interleukin （IL）-12 （5 μg/kg every other day） was given for 7 days. The number of CD8＋ effector memory T-cells （Tern）, expression of interferon （IFN）-y, roTOR, and ribosomal protein $6 kinase （S6K）, and the levels of IL-6, IL- 10, galactomannan （GM）, and soluble TREM- 1 （sTREM-I） were measured. Results： Viable A. fumigatus was cultured from the lung tissue of the inoculated mice. Histological examination indicated greater inflammation, hemorrhage, and lung tissue injury in both IPA and CTX ＋ IPA mice groups. The expression of mTOR and S6K was significantly increased in the CTX ＋ IPA ＋ I L- 12 group compared with the control, I PA （P = 0.01 ; P - 0.001 ）, and CTX ＋ 1PA （P = 0.034; P = 0.032） groups, but significantly decreased in the CTX ＋ IPA ＋ RAPA group （P 〈 0.001 ）. Compared with the CTX ＋ IPA group, the proportion of Tern, expression of IFN-y, and the level ofsTREM-I were significantly higher after IL-12 treatment （P = 0.024, P = 0.032, and P = 0.017, respectively）, and the opposite results were observed when the roTOR pathway was blocked by rapamycin （P 〈 0.001）. Compared with the CTX ＋ I PA and CTX ＋ I PA ＋ RAPA groups, IL-12 treatment increased IL-6
