In this study, rats were put into traumatic brain injury-induced coma and treated with median nerve electrical stimulation. We explored the wake-promoting effect, and possible mechanisms, of median nerve electrical st...In this study, rats were put into traumatic brain injury-induced coma and treated with median nerve electrical stimulation. We explored the wake-promoting effect, and possible mechanisms, of median nerve electrical stimulation. Electrical stimulation upregulated the expression levels of orexin-A and its receptor OX1R in the rat prefrontal cortex. Orexin-A expression gradually in-creased with increasing stimulation, while OX1R expression reached a peak at 12 hours and then decreased. In addition, after the OX1R antagonist, SB334867, was injected into the brain of rats after traumatic brain injury, fewer rats were restored to consciousness, and orexin-A and OXIR expression in the prefrontal cortex was downregulated. Our ifndings indicate that median nerve electrical stimulation induced an up-regulation of orexin-A and OX1R expression in the pre-frontal cortex of traumatic brain injury-induced coma rats, which may be a potential mechanism involved in the wake-promoting effects of median nerve electrical stimulation.展开更多
目的评估早期肠内营养(EEN)对重症颅脑损伤患者炎症反应的影响。方法选取2012年7月到2014年7月我院外科ICU和神经外科收治的重症颅脑损伤患者140例,将患者随机分为研究组和对照组,对照组给予肠外营养(PN),研究组给予EEN,记录两组患者治...目的评估早期肠内营养(EEN)对重症颅脑损伤患者炎症反应的影响。方法选取2012年7月到2014年7月我院外科ICU和神经外科收治的重症颅脑损伤患者140例,将患者随机分为研究组和对照组,对照组给予肠外营养(PN),研究组给予EEN,记录两组患者治疗1、3、5、7、14 d血清C反应蛋白(CRP)水平和格拉斯哥昏迷评分(GCS);记录两组患者治疗7 d及14 d血清总蛋白、血清白蛋白、外周淋巴细胞计数。结果治疗后两组CRP均下降,治疗1 d两组CRP比较差异无统计学意义(P>0.05),研究组3、5、7、14 d CRP低于对照组,两组比较差异有统计学意义(P<0.05);两组1、3、5、7 d GCS比较差异无统计学意义(P>0.05),第14天研究组GCS评分高于对照组,两组比较差异有统计学意义(P<0.05)。两组治疗后第7天及第14天血清总蛋白、血清白蛋白、外周淋巴细胞计数均有上升,上述指标研究组高于对照组,两组比较差异有统计学差异(P<0.05)。结论 EEN与PN相比减轻了重症颅脑损伤患者的炎症反应,有助于患者从昏迷中恢复,改善了患者机体的内环境。展开更多
基金funded by grants from the National Natural Science Foundation of China,No.81260295the Natural Science Foundation of Jiangxi Province of China,No.20132BAB205063
文摘In this study, rats were put into traumatic brain injury-induced coma and treated with median nerve electrical stimulation. We explored the wake-promoting effect, and possible mechanisms, of median nerve electrical stimulation. Electrical stimulation upregulated the expression levels of orexin-A and its receptor OX1R in the rat prefrontal cortex. Orexin-A expression gradually in-creased with increasing stimulation, while OX1R expression reached a peak at 12 hours and then decreased. In addition, after the OX1R antagonist, SB334867, was injected into the brain of rats after traumatic brain injury, fewer rats were restored to consciousness, and orexin-A and OXIR expression in the prefrontal cortex was downregulated. Our ifndings indicate that median nerve electrical stimulation induced an up-regulation of orexin-A and OX1R expression in the pre-frontal cortex of traumatic brain injury-induced coma rats, which may be a potential mechanism involved in the wake-promoting effects of median nerve electrical stimulation.
文摘目的评估早期肠内营养(EEN)对重症颅脑损伤患者炎症反应的影响。方法选取2012年7月到2014年7月我院外科ICU和神经外科收治的重症颅脑损伤患者140例,将患者随机分为研究组和对照组,对照组给予肠外营养(PN),研究组给予EEN,记录两组患者治疗1、3、5、7、14 d血清C反应蛋白(CRP)水平和格拉斯哥昏迷评分(GCS);记录两组患者治疗7 d及14 d血清总蛋白、血清白蛋白、外周淋巴细胞计数。结果治疗后两组CRP均下降,治疗1 d两组CRP比较差异无统计学意义(P>0.05),研究组3、5、7、14 d CRP低于对照组,两组比较差异有统计学意义(P<0.05);两组1、3、5、7 d GCS比较差异无统计学意义(P>0.05),第14天研究组GCS评分高于对照组,两组比较差异有统计学意义(P<0.05)。两组治疗后第7天及第14天血清总蛋白、血清白蛋白、外周淋巴细胞计数均有上升,上述指标研究组高于对照组,两组比较差异有统计学差异(P<0.05)。结论 EEN与PN相比减轻了重症颅脑损伤患者的炎症反应,有助于患者从昏迷中恢复,改善了患者机体的内环境。
