微焦点X射线源相衬成像是利用微焦点X射线源透过样品后携带的相位信息对样品内部结构成像．通过获取相面上的强度信息利用相位复原方法可以得到物体内部的相位信息．获取了不同放大倍数下的蚂蚁图像,通过强度传播方程(Transport of Inte...微焦点X射线源相衬成像是利用微焦点X射线源透过样品后携带的相位信息对样品内部结构成像．通过获取相面上的强度信息利用相位复原方法可以得到物体内部的相位信息．获取了不同放大倍数下的蚂蚁图像,通过强度传播方程(Transport of Intensity Equation,TIE)方法得到了蚂蚁的相衬图像,采用边缘可见度(Edge Visibility)方法和熵法比较了不同放大倍数下的成像效果以及复原前后图像质量．展开更多
Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced pla...Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced planes.The advantage of a TIE-based QPI system is its compatibility with partially coherent illumination,which provides speckle-free imaging with resolution beyond the coherent diffraction limit.However,TIE is generally implemented with a brightfield(BF)configuration,and the maximum achievable imaging resolution is still limited to the incoherent diffraction limit(twice the coherent diffraction limit).It is desirable that TIE-related approaches can surpass this limit and achieve high-throughput[high-resolution and wide field of view(FOV)]QPI.We propose a hybrid BF and darkfield transport of intensity(HBDTI)approach for highthroughput quantitative phase microscopy.Two through-focus intensity stacks corresponding to BF and darkfield illuminations are acquired through a low-numerical-aperture(NA)objective lens.The high-resolution and large-FOV complex amplitude(both quantitative absorption and phase distributions)can then be synthesized based on an iterative phase retrieval algorithm taking the coherence model decomposition into account.The effectiveness of the proposed method is experimentally verified by the retrieval of the USAF resolution target and different types of biological cells.The experimental results demonstrate that the half-width imaging resolution can be improved from 1230 nm to 488 nm with 2.5×expansion across a 4×FOV of 7.19 mm2,corresponding to a 6.25×increase in space-bandwidth product from∼5 to∼30.2 megapixels.In contrast to conventional TIE-based QPI methods where only BF illumination is used,the synthetic aperture process of HBDTI further incorporates darkfield illuminations to expand the accessible object frequency,thereby significantly extending the maximum available resolution from 2NA to∼5NA with a∼5×promotion of the coherent diffraction limit.Given its capability for展开更多
Non-interferometric X-ray quantitative phase imaging(XQPI),much simpler than the interferometric scheme,has provided high-resolution and reliable phase-contrast images.We report on implementing the volumetric XQPI ima...Non-interferometric X-ray quantitative phase imaging(XQPI),much simpler than the interferometric scheme,has provided high-resolution and reliable phase-contrast images.We report on implementing the volumetric XQPI images using concurrent-bidirectional scanning of the orthogonal plane on the optical axis of the Foucault differential filter;we then extracted data in conjunction with the transport-intensity equation.The volumetric image of the laminate microstructure of the gills of a fish was successfully reconstructed to demonstrate our XQPI method.The method can perform 3D rendering without any rotational motion for laterally extended objects by manipulating incoherent X-rays using the pinhole array.展开更多
文摘微焦点X射线源相衬成像是利用微焦点X射线源透过样品后携带的相位信息对样品内部结构成像．通过获取相面上的强度信息利用相位复原方法可以得到物体内部的相位信息．获取了不同放大倍数下的蚂蚁图像,通过强度传播方程(Transport of Intensity Equation,TIE)方法得到了蚂蚁的相衬图像,采用边缘可见度(Edge Visibility)方法和熵法比较了不同放大倍数下的成像效果以及复原前后图像质量．
基金the National Natural Science Foundation of China(61905115,62105151,62175109,and U21B2033)Leading Technology of Jiangsu Basic Research Plan(BK20192003)+2 种基金Youth Foundation of Jiangsu Province(BK20190445,BK20210338)Fundamental Research Funds for the Central Universities(30920032101)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging and Intelligent Sense(JSGP202105).
文摘Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced planes.The advantage of a TIE-based QPI system is its compatibility with partially coherent illumination,which provides speckle-free imaging with resolution beyond the coherent diffraction limit.However,TIE is generally implemented with a brightfield(BF)configuration,and the maximum achievable imaging resolution is still limited to the incoherent diffraction limit(twice the coherent diffraction limit).It is desirable that TIE-related approaches can surpass this limit and achieve high-throughput[high-resolution and wide field of view(FOV)]QPI.We propose a hybrid BF and darkfield transport of intensity(HBDTI)approach for highthroughput quantitative phase microscopy.Two through-focus intensity stacks corresponding to BF and darkfield illuminations are acquired through a low-numerical-aperture(NA)objective lens.The high-resolution and large-FOV complex amplitude(both quantitative absorption and phase distributions)can then be synthesized based on an iterative phase retrieval algorithm taking the coherence model decomposition into account.The effectiveness of the proposed method is experimentally verified by the retrieval of the USAF resolution target and different types of biological cells.The experimental results demonstrate that the half-width imaging resolution can be improved from 1230 nm to 488 nm with 2.5×expansion across a 4×FOV of 7.19 mm2,corresponding to a 6.25×increase in space-bandwidth product from∼5 to∼30.2 megapixels.In contrast to conventional TIE-based QPI methods where only BF illumination is used,the synthetic aperture process of HBDTI further incorporates darkfield illuminations to expand the accessible object frequency,thereby significantly extending the maximum available resolution from 2NA to∼5NA with a∼5×promotion of the coherent diffraction limit.Given its capability for
基金supported by the National Research Foundation of Korea(NRF)funded by the Korean government(MEST)(No.2021R1C1C200514).
文摘Non-interferometric X-ray quantitative phase imaging(XQPI),much simpler than the interferometric scheme,has provided high-resolution and reliable phase-contrast images.We report on implementing the volumetric XQPI images using concurrent-bidirectional scanning of the orthogonal plane on the optical axis of the Foucault differential filter;we then extracted data in conjunction with the transport-intensity equation.The volumetric image of the laminate microstructure of the gills of a fish was successfully reconstructed to demonstrate our XQPI method.The method can perform 3D rendering without any rotational motion for laterally extended objects by manipulating incoherent X-rays using the pinhole array.
