We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltratio...We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.展开更多
以转lrp(lysine-rich protein gene)基因的转基因T0和T1代生菜为材料,非转基因生菜植株为对照,研究包括赖氨酸在内的所有氨基酸的变化情况。结果表明:lrp可以在生菜体内正常表达,在2个世代都可提高生菜中赖氨酸的含量,同时,其他必需氨...以转lrp(lysine-rich protein gene)基因的转基因T0和T1代生菜为材料,非转基因生菜植株为对照,研究包括赖氨酸在内的所有氨基酸的变化情况。结果表明:lrp可以在生菜体内正常表达,在2个世代都可提高生菜中赖氨酸的含量,同时,其他必需氨基酸的含量并没有变化。展开更多
基金Project supported by the National Key Technology Research and Development Program of China (No. 2007BAD59B06)the Interna-tional Science and Technology Cooperation Program of China (No. 2007DFA31260)the Science Foundation for the Excellent Youth Scholars of Guizhou Province, China (No. 20030312)
文摘We investigated the possibility of producing chicken alpha interferon （ChlFN-α） in transgenic plants. The cDNA encoding ChlFN-α was introduced into lettuce （Lactuca sativa L.） plants by using an agro-infiltration transient expression system. The ChlFN-a gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus （VSV） replication on chicken embryonic fibroblast （CEF）. The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.
