Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underly- ing mechanisms remain unclear. The duration of heat-sensitive suspended moxibusti...Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underly- ing mechanisms remain unclear. The duration of heat-sensitive suspended moxibustion （usually from 30 minutes to 1 hour） is longer than traditional suspended moxibustion （usually 15 minutes）. However, the effects of 15- and 35-minute suspended moxibustion in rats with cerebra/ischemia/reperfusion injury are poorly understood. In this study, we performed 15- or 35-minute suspended moxibustion at acupoint Dazhui （GV14） in an adult rat model of focal cerebral ischemia/reperfusion injury. Infarct volume was evaluated with the 2,3,5-triphenyltetrazolium chloride assay. Histopathological changes and neuronal apoptosis at the injury site were assessed by hematoxy- lin-eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Caspase-9 and caspase-3 expression at the in- jury site was detected using immunofluorescent staining. Bax and Bcl-2 expression at the injury site was assessed using western blot assay. In the 35-minute moxibustion group, infarct volume was decreased, neuronal apoptosis was reduced, caspase-9, caspase-3 and Bax expres- sion was lower, and Bcl-2 expression was increased, compared with the 15-minute moxibustion group. Our findings show that 35-minute moxibustion has a greater anti-apoptotic effect than 15-minute moxibustion after focal cerebral ischemia/reperfusion injury.展开更多
目的观察参苓白术散加减辅助治疗2型糖尿病伴肥胖症的临床疗效。方法选取符合纳入标准的2型糖尿病伴肥胖症患者80例,随机分为观察组和对照组各40例,两组均给予胰岛素治疗(三短一长),观察组在胰岛素治疗基础上加服参苓白术散加减方,疗程1...目的观察参苓白术散加减辅助治疗2型糖尿病伴肥胖症的临床疗效。方法选取符合纳入标准的2型糖尿病伴肥胖症患者80例,随机分为观察组和对照组各40例,两组均给予胰岛素治疗(三短一长),观察组在胰岛素治疗基础上加服参苓白术散加减方,疗程12周,观察两组治疗前后空腹血糖(FPG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(Hb A1c)、体重指数(BMI)、腰臀比(WHR),评判疗效。结果观察组患者治疗后的FPG、2 h PG、Hb A1c、BMI、WHR明显好于对照组,差异均有统计学意义(P<0.05),且服药期间无明显不良反应,依从性好。结论参苓白术散加减方可有助降低血糖、减轻体重,对于2型糖尿病伴肥胖症患者,具有较好疗效。展开更多
基金supported by the National Natural Science Foundation of China,No.81060305&81660819the Natural Science Foundation of Jiangxi Province of China,No.2015BAB205068+2 种基金Key Program for Science and Technology Cooperation Projects of Jiangxi Province of China,No.20161BBH80053a grant from the Key Project of Health Commission of Jiangxi Province of China,No.2014Z003the Natural Science Foundation of Jiangxi University of Traditional Chinese Medicine of China,No.2014ZR018&2015jzzdxk024
文摘Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underly- ing mechanisms remain unclear. The duration of heat-sensitive suspended moxibustion （usually from 30 minutes to 1 hour） is longer than traditional suspended moxibustion （usually 15 minutes）. However, the effects of 15- and 35-minute suspended moxibustion in rats with cerebra/ischemia/reperfusion injury are poorly understood. In this study, we performed 15- or 35-minute suspended moxibustion at acupoint Dazhui （GV14） in an adult rat model of focal cerebral ischemia/reperfusion injury. Infarct volume was evaluated with the 2,3,5-triphenyltetrazolium chloride assay. Histopathological changes and neuronal apoptosis at the injury site were assessed by hematoxy- lin-eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Caspase-9 and caspase-3 expression at the in- jury site was detected using immunofluorescent staining. Bax and Bcl-2 expression at the injury site was assessed using western blot assay. In the 35-minute moxibustion group, infarct volume was decreased, neuronal apoptosis was reduced, caspase-9, caspase-3 and Bax expres- sion was lower, and Bcl-2 expression was increased, compared with the 15-minute moxibustion group. Our findings show that 35-minute moxibustion has a greater anti-apoptotic effect than 15-minute moxibustion after focal cerebral ischemia/reperfusion injury.
文摘目的观察参苓白术散加减辅助治疗2型糖尿病伴肥胖症的临床疗效。方法选取符合纳入标准的2型糖尿病伴肥胖症患者80例,随机分为观察组和对照组各40例,两组均给予胰岛素治疗(三短一长),观察组在胰岛素治疗基础上加服参苓白术散加减方,疗程12周,观察两组治疗前后空腹血糖(FPG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(Hb A1c)、体重指数(BMI)、腰臀比(WHR),评判疗效。结果观察组患者治疗后的FPG、2 h PG、Hb A1c、BMI、WHR明显好于对照组,差异均有统计学意义(P<0.05),且服药期间无明显不良反应,依从性好。结论参苓白术散加减方可有助降低血糖、减轻体重,对于2型糖尿病伴肥胖症患者,具有较好疗效。
