Thrips are among the most important agricultural pests globally because of the damage inflicted by their oviposition, feeding, and ability to transmit plant viruses. Because of their invasiveness, a number of pest spe...Thrips are among the most important agricultural pests globally because of the damage inflicted by their oviposition, feeding, and ability to transmit plant viruses. Because of their invasiveness, a number of pest species are common to both China and the United States and present significant challenges to growers of a wide range of crops in both countries. Among the pest thrips common to both countries are four of the major global thrips pests, Frankliniella occidentalis （Pergande）, Scirtothrips dorsalis Hood, Thrips palmi Karny, and Thrips tabaci Lindeman. This review addresses characteristics that enable thrips to be such damaging pests and how biological attributes of thrips create challenges for their management. Despite these challenges, a number of successful management tactics have been developed for various cropping systems. We discuss some of these tactics that have been developed, including the use of cultural controls, biological controls, and judicious use of insecticides that do not disrupt overall pest management programs. The exchange of this type of information will help to facilitate management of pest thrips, especially in regions where species have recently invaded. A prime example is F. occidentalis, the western flower thrips, which is native to the United States, but has recently invaded China. Therefore, management tactics developed in the United States can be adapted to China. Because further success in management of thrips requires a thorough understanding of thrips ecology, we discuss areas of future research and emphasize the importance of collaboration among different countries to enhance our overall understanding of the biology and ecology of thrips and to improve management programs for these widespread pests.展开更多
Some tomato samples possibly infected by tospovirus in Guangdong were detected with indirect ELISA and RT-PCR.The results showed that the virus infected tomato did not react with the antiserum of Tomato spotted wilt v...Some tomato samples possibly infected by tospovirus in Guangdong were detected with indirect ELISA and RT-PCR.The results showed that the virus infected tomato did not react with the antiserum of Tomato spotted wilt virus(TSWV),but about 500 bp fragment of RT-PCR shared 83%-84% nucleotide identities with N gene of those reported tospoviruses.The phylogenetic tree of the N gene fragment compared with those of other tospoviruses indicated that the virus infected tomato was belonged to Tospovirus.展开更多
2004-2007年间对云南省烤烟种植区进行调查，在云南省楚雄和文山2个地区，获得3份疑似Tospovirus属病毒的样本。通过Tospovirus组引物（Tospovirus Group Primer）进行RT-PCR扩增、克隆和测序，获得500nts核酸片段，通过NCBI BLAST网络...2004-2007年间对云南省烤烟种植区进行调查，在云南省楚雄和文山2个地区，获得3份疑似Tospovirus属病毒的样本。通过Tospovirus组引物（Tospovirus Group Primer）进行RT-PCR扩增、克隆和测序，获得500nts核酸片段，通过NCBI BLAST网络数据库比对，确定这3份样品为Tospovirus属的病毒；通过DNAman软件比对分析所得到的核酸序列和已经公布的Tospovirus属病毒RNA L同位点序列，从得到的系统进化图可以看出，云南3个样品明显分为2簇，即YN-1和YN-2，分别与血清组I和Ⅵ的Tospovirus属病毒聚为一簇。展开更多
基金supported by the National Basic Research Program of China (973 Program, 2009CB119004)the National Special Fund for the Commonweal Agricultural Research of China (200903032)the Earmarked Fund for Modern Agro-industry Technology Research System, China (Nycytx-35-gw27)
文摘Thrips are among the most important agricultural pests globally because of the damage inflicted by their oviposition, feeding, and ability to transmit plant viruses. Because of their invasiveness, a number of pest species are common to both China and the United States and present significant challenges to growers of a wide range of crops in both countries. Among the pest thrips common to both countries are four of the major global thrips pests, Frankliniella occidentalis （Pergande）, Scirtothrips dorsalis Hood, Thrips palmi Karny, and Thrips tabaci Lindeman. This review addresses characteristics that enable thrips to be such damaging pests and how biological attributes of thrips create challenges for their management. Despite these challenges, a number of successful management tactics have been developed for various cropping systems. We discuss some of these tactics that have been developed, including the use of cultural controls, biological controls, and judicious use of insecticides that do not disrupt overall pest management programs. The exchange of this type of information will help to facilitate management of pest thrips, especially in regions where species have recently invaded. A prime example is F. occidentalis, the western flower thrips, which is native to the United States, but has recently invaded China. Therefore, management tactics developed in the United States can be adapted to China. Because further success in management of thrips requires a thorough understanding of thrips ecology, we discuss areas of future research and emphasize the importance of collaboration among different countries to enhance our overall understanding of the biology and ecology of thrips and to improve management programs for these widespread pests.
文摘Some tomato samples possibly infected by tospovirus in Guangdong were detected with indirect ELISA and RT-PCR.The results showed that the virus infected tomato did not react with the antiserum of Tomato spotted wilt virus(TSWV),but about 500 bp fragment of RT-PCR shared 83%-84% nucleotide identities with N gene of those reported tospoviruses.The phylogenetic tree of the N gene fragment compared with those of other tospoviruses indicated that the virus infected tomato was belonged to Tospovirus.
文摘2004-2007年间对云南省烤烟种植区进行调查，在云南省楚雄和文山2个地区，获得3份疑似Tospovirus属病毒的样本。通过Tospovirus组引物（Tospovirus Group Primer）进行RT-PCR扩增、克隆和测序，获得500nts核酸片段，通过NCBI BLAST网络数据库比对，确定这3份样品为Tospovirus属的病毒；通过DNAman软件比对分析所得到的核酸序列和已经公布的Tospovirus属病毒RNA L同位点序列，从得到的系统进化图可以看出，云南3个样品明显分为2簇，即YN-1和YN-2，分别与血清组I和Ⅵ的Tospovirus属病毒聚为一簇。
