[Objectives]To optimize the forming process of Yi medicine Tongfeng Granules.[Methods]The forming process of Yi medicine Tongfeng Granules was optimized,with paste density,ethanol volume fraction,and type and proporti...[Objectives]To optimize the forming process of Yi medicine Tongfeng Granules.[Methods]The forming process of Yi medicine Tongfeng Granules was optimized,with paste density,ethanol volume fraction,and type and proportion of excipient as influencing factors,and granule formability,solubility,moisture absorption,and angle of repose as evaluation indicators.Critical relative humidity(CRH)was investigated to select optimal storage conditions.[Results]Maltodextrin was selected as the excipient,and the best process parameters was the ratio of drug to excipient at 1∶2(g/g),under which the forming rate,solubility,moisture absorption rate,and angle of repose were 81.38%,98.90%,8.81%,and 27.5°,respectively.The critical relative humidity was 72%.[Conclusions]The forming process adopted is reasonable and feasible,and can provide a reference for large-scale production of Yi medicine Tongfeng Granules.展开更多
目的:观察清热除湿通络法对大鼠痛风性关节炎模型中NALP3、Caspase-1、Pycard m RNA及NALP3、Caspase-1蛋白表达的影响。方法:将经典痛风性关节炎模型大鼠造模后采用随机数表法分为空白组、模型组、虎杖组及其各拆方组、吲哚美辛组和秋...目的:观察清热除湿通络法对大鼠痛风性关节炎模型中NALP3、Caspase-1、Pycard m RNA及NALP3、Caspase-1蛋白表达的影响。方法:将经典痛风性关节炎模型大鼠造模后采用随机数表法分为空白组、模型组、虎杖组及其各拆方组、吲哚美辛组和秋水仙碱组。造模12h后取材,随后运用RT-PCR技术检测大鼠气囊灌洗液中NALP3、Caspase-1、Pycard m RNA变化;Western blot法检测大鼠气囊灌洗液中NALP3、Caspase-1蛋白的变化。结果:以模型组为对照,虎杖组NALP3 m RNA相对表达下调,而其余组均上调;虎杖组Caspase-1 m RNA上调幅度小于其余组(除清热方组);虎杖组Pycard m RNA表达上调幅度低于其余组。虎杖组NALP3蛋白表达水平较模型组及其各拆方组、秋水仙碱组低(P<0.01,P<0.05);虎杖组Caspase-1蛋白表达量低于除湿方组、模型组(P<0.05,P<0.01)。结论:清热除湿通络法通过在蛋白质翻译层面下调NALP3、Caspase-1蛋白,干扰了NALP3炎性复合体的装配或激活,进而发挥了拮抗痛风炎性反应的作用。展开更多
基金National Key R&D Program(2018YFC1708005)Sichuan Provincial Key R&D Project(2021YFS0043)Fundamental Research Funds for the Central Universities(2020NGD01).
文摘[Objectives]To optimize the forming process of Yi medicine Tongfeng Granules.[Methods]The forming process of Yi medicine Tongfeng Granules was optimized,with paste density,ethanol volume fraction,and type and proportion of excipient as influencing factors,and granule formability,solubility,moisture absorption,and angle of repose as evaluation indicators.Critical relative humidity(CRH)was investigated to select optimal storage conditions.[Results]Maltodextrin was selected as the excipient,and the best process parameters was the ratio of drug to excipient at 1∶2(g/g),under which the forming rate,solubility,moisture absorption rate,and angle of repose were 81.38%,98.90%,8.81%,and 27.5°,respectively.The critical relative humidity was 72%.[Conclusions]The forming process adopted is reasonable and feasible,and can provide a reference for large-scale production of Yi medicine Tongfeng Granules.
文摘目的:观察清热除湿通络法对大鼠痛风性关节炎模型中NALP3、Caspase-1、Pycard m RNA及NALP3、Caspase-1蛋白表达的影响。方法:将经典痛风性关节炎模型大鼠造模后采用随机数表法分为空白组、模型组、虎杖组及其各拆方组、吲哚美辛组和秋水仙碱组。造模12h后取材,随后运用RT-PCR技术检测大鼠气囊灌洗液中NALP3、Caspase-1、Pycard m RNA变化;Western blot法检测大鼠气囊灌洗液中NALP3、Caspase-1蛋白的变化。结果:以模型组为对照,虎杖组NALP3 m RNA相对表达下调,而其余组均上调;虎杖组Caspase-1 m RNA上调幅度小于其余组(除清热方组);虎杖组Pycard m RNA表达上调幅度低于其余组。虎杖组NALP3蛋白表达水平较模型组及其各拆方组、秋水仙碱组低(P<0.01,P<0.05);虎杖组Caspase-1蛋白表达量低于除湿方组、模型组(P<0.05,P<0.01)。结论:清热除湿通络法通过在蛋白质翻译层面下调NALP3、Caspase-1蛋白,干扰了NALP3炎性复合体的装配或激活,进而发挥了拮抗痛风炎性反应的作用。
