Two catechin epimers and their non-covalent complexes with γ-cyclodextrin were studied by using ion mobility coupled with mass spectrometry (IM-MS). Rapid separation of complexes was achieved with the peak-to-peak re...Two catechin epimers and their non-covalent complexes with γ-cyclodextrin were studied by using ion mobility coupled with mass spectrometry (IM-MS). Rapid separation of complexes was achieved with the peak-to-peak resolution reaching 0.86 after optimization of IM condition. Collision cross section (CCS) was measured to explore the structural difference of complexes. A gap of 11.75 A^2 between two complexes was found. Molecular modeling and theoretical CCS calculation were adopted to explain the measurement results. Two binding ways of both complexes were found and the calculated CCS corresponds accurately to the measured CCS. Quantification of catechins in mixtures was performed and the relative error was less than 15%, indicating the effectiveness of quantification by IM-MS.展开更多
基金the National Natural Science Foundation of China (Nos.21673219 and 81873193).
文摘Two catechin epimers and their non-covalent complexes with γ-cyclodextrin were studied by using ion mobility coupled with mass spectrometry (IM-MS). Rapid separation of complexes was achieved with the peak-to-peak resolution reaching 0.86 after optimization of IM condition. Collision cross section (CCS) was measured to explore the structural difference of complexes. A gap of 11.75 A^2 between two complexes was found. Molecular modeling and theoretical CCS calculation were adopted to explain the measurement results. Two binding ways of both complexes were found and the calculated CCS corresponds accurately to the measured CCS. Quantification of catechins in mixtures was performed and the relative error was less than 15%, indicating the effectiveness of quantification by IM-MS.
