建立了液液萃取-分散液液微萃取-气相色谱-质谱联用技术测定纺织废水中痕量偶氮染料的方法。废水中的偶氮染料在碱性条件下经连二亚硫酸钠还原成芳香胺后,先用叔丁基甲醚液液萃取、盐酸反萃进行预浓缩及净化;再以乙腈-氯苯体系进行分散...建立了液液萃取-分散液液微萃取-气相色谱-质谱联用技术测定纺织废水中痕量偶氮染料的方法。废水中的偶氮染料在碱性条件下经连二亚硫酸钠还原成芳香胺后,先用叔丁基甲醚液液萃取、盐酸反萃进行预浓缩及净化;再以乙腈-氯苯体系进行分散液液微萃取,气相色谱-质谱测定。对前处理条件进行了优化,考察了酸碱度及盐效应对芳香胺萃取效率的影响,结果表明:液液萃取过程中加入30 g NaCl,分散液液微萃取过程中加入1 mL 5 mol/L的NaOH调节体系至碱性才能达到较好的萃取效率。在优化的实验条件下,21种目标物均呈现良好的线性关系,其中13种芳香胺的线性范围为0.05~10μg/L,7种芳香胺的线性范围为0.05~5μg/L,2,4-二氨基苯甲醚的线性范围为20~100μg/L,相关系数为0.996~0.999。20种芳香胺的检出限可达0.05μg/L,2,4-二氨基苯甲醚检出限为20μg/L。印染、机织、印花等实际废水加标试验表明,方法的回收率为75.6%~115.1%。该方法富集倍数高,检出限低,适用于纺织废水中痕量禁用偶氮染料的检测。展开更多
The study aims to isolate and optimize bacterial strains having the ability to degrade and decolorize azo dyes produced in the final effluent of textile dying industries. In this regard, ten bacterial strains were iso...The study aims to isolate and optimize bacterial strains having the ability to degrade and decolorize azo dyes produced in the final effluent of textile dying industries. In this regard, ten bacterial strains were isolated from wastewater treatment plants, and most of them were subjected to the colored effluents resulting from dilapidated houses. The ability of these bacterial isolations to use a wide range of azo dyes to determine the sole carbon source was determined. According to these screening testes, two bacterial isolations were selected as the most potent decolorizer for azo dyes, and they were identified as Comamanas acidovorns-TN1 and Burkholdera cepace-TN5. The optimization process started with the addition of 1 g/l yeast extract, where the decolorization ability of the two strains increased sharply and according to this experiment, the two azo dyes, Acid orange 7 and Direct blue 75, were selected to complete the study. The effect of different conditional and chemical factors on the decolorization process of Acid orange 7 and Direct blue 75 by Comamanas acidovorns-TN1 and Burkholdera cepace-TN5 was studied. Factors that contributed to the difference were different pH, temperature, incubation period, inoculum size, carbon source, nitrogen source and the respective concentrations of yeast extract. This study recommends the application of the two most potent bacterial strains in the decolorization of the azo dyes, along with acid orange 7 and direct blue 75, specifically in the industrial effluents under all nutritional and environmental conditions.展开更多
文摘建立了液液萃取-分散液液微萃取-气相色谱-质谱联用技术测定纺织废水中痕量偶氮染料的方法。废水中的偶氮染料在碱性条件下经连二亚硫酸钠还原成芳香胺后,先用叔丁基甲醚液液萃取、盐酸反萃进行预浓缩及净化;再以乙腈-氯苯体系进行分散液液微萃取,气相色谱-质谱测定。对前处理条件进行了优化,考察了酸碱度及盐效应对芳香胺萃取效率的影响,结果表明:液液萃取过程中加入30 g NaCl,分散液液微萃取过程中加入1 mL 5 mol/L的NaOH调节体系至碱性才能达到较好的萃取效率。在优化的实验条件下,21种目标物均呈现良好的线性关系,其中13种芳香胺的线性范围为0.05~10μg/L,7种芳香胺的线性范围为0.05~5μg/L,2,4-二氨基苯甲醚的线性范围为20~100μg/L,相关系数为0.996~0.999。20种芳香胺的检出限可达0.05μg/L,2,4-二氨基苯甲醚检出限为20μg/L。印染、机织、印花等实际废水加标试验表明,方法的回收率为75.6%~115.1%。该方法富集倍数高,检出限低,适用于纺织废水中痕量禁用偶氮染料的检测。
文摘The study aims to isolate and optimize bacterial strains having the ability to degrade and decolorize azo dyes produced in the final effluent of textile dying industries. In this regard, ten bacterial strains were isolated from wastewater treatment plants, and most of them were subjected to the colored effluents resulting from dilapidated houses. The ability of these bacterial isolations to use a wide range of azo dyes to determine the sole carbon source was determined. According to these screening testes, two bacterial isolations were selected as the most potent decolorizer for azo dyes, and they were identified as Comamanas acidovorns-TN1 and Burkholdera cepace-TN5. The optimization process started with the addition of 1 g/l yeast extract, where the decolorization ability of the two strains increased sharply and according to this experiment, the two azo dyes, Acid orange 7 and Direct blue 75, were selected to complete the study. The effect of different conditional and chemical factors on the decolorization process of Acid orange 7 and Direct blue 75 by Comamanas acidovorns-TN1 and Burkholdera cepace-TN5 was studied. Factors that contributed to the difference were different pH, temperature, incubation period, inoculum size, carbon source, nitrogen source and the respective concentrations of yeast extract. This study recommends the application of the two most potent bacterial strains in the decolorization of the azo dyes, along with acid orange 7 and direct blue 75, specifically in the industrial effluents under all nutritional and environmental conditions.
