Chimaeric TA29 Barnase gene was introduced into oilseed rape (Brassica napus) of good quality and high yield by Agrobacterium tumefaciens transformation.The transgenic plants were obtained and transformed genome was d...Chimaeric TA29 Barnase gene was introduced into oilseed rape (Brassica napus) of good quality and high yield by Agrobacterium tumefaciens transformation.The transgenic plants were obtained and transformed genome was determined by Southern blot analysis.About 90% TA29 Barnase transgenic plants were male sterile.However,about 80% transgenic plants turned to be male fertile at temperature higher than 25 ℃.It suggested that male sterility of these transgenic plants was probably temperature sensitive.展开更多
Poly( N isopropylacrylamide)/poly(vinyl alcohol)(PNV) hydrogels were prepared by repetitive freezing and thawing. This kind of hydrogels was formed through physical crosslinking, i.e. hydrogen bonds during the freezin...Poly( N isopropylacrylamide)/poly(vinyl alcohol)(PNV) hydrogels were prepared by repetitive freezing and thawing. This kind of hydrogels was formed through physical crosslinking, i.e. hydrogen bonds during the freezing process. The swelling ratio and deswelling/reswelling kinetics were measured gravimetrically and this novel hydrogel exhibits a temperature sensitivity. This hydrogel may be useful both in biomedical and biotechnological fields.展开更多
文摘Chimaeric TA29 Barnase gene was introduced into oilseed rape (Brassica napus) of good quality and high yield by Agrobacterium tumefaciens transformation.The transgenic plants were obtained and transformed genome was determined by Southern blot analysis.About 90% TA29 Barnase transgenic plants were male sterile.However,about 80% transgenic plants turned to be male fertile at temperature higher than 25 ℃.It suggested that male sterility of these transgenic plants was probably temperature sensitive.
文摘Poly( N isopropylacrylamide)/poly(vinyl alcohol)(PNV) hydrogels were prepared by repetitive freezing and thawing. This kind of hydrogels was formed through physical crosslinking, i.e. hydrogen bonds during the freezing process. The swelling ratio and deswelling/reswelling kinetics were measured gravimetrically and this novel hydrogel exhibits a temperature sensitivity. This hydrogel may be useful both in biomedical and biotechnological fields.
