Herein,a colorimetric chemosensor(probe 7)was designed and synthesized for sensing diethyl cyanophosphonate(DCNP),a Tabun nerve agent simulant.The sensitive,selective,fast response and photostable probe 7 in dimethyls...Herein,a colorimetric chemosensor(probe 7)was designed and synthesized for sensing diethyl cyanophosphonate(DCNP),a Tabun nerve agent simulant.The sensitive,selective,fast response and photostable probe 7 in dimethylsulfoxide(DMSO)responded to DCNP at low concentrations with visual naked eye color change from pink to pale yellow and displayed unresponsive behaviour against various interferences except for copper ions,which can be differentiated by adding ethylene diamine where the yellow color persisted in the case of DCNP but turned to pink in the case of copper ions.Additionally,the yellow aqueous solution of probe 7 at pH 10 and 12 turned colorless against the response of DCNP.The limit of detection(LOD)of probe 7 was found to be 50 nmol/L in an aqueous basic solution at pH 10 for the detection of DCNP.The formation of the cyclized product was elucidated by 1H and 31P NMR nuclear magnetic resonance(1H NMR,31P NMR),Fourier transform infrared spectroscopy(FTIR),and high-resolution mass spectrometry(HRMS)analysis.The filter paper and silica plate loaded with probe 7 were fabricated for real-time and on-site detection of DCNP.展开更多
The main aim for discovery and development of the neurophysiological detector was detection of the production’ seats and criminal use of poisons in warfare. Phosphor-organic (PO) substances with acetylcholinesterase-...The main aim for discovery and development of the neurophysiological detector was detection of the production’ seats and criminal use of poisons in warfare. Phosphor-organic (PO) substances with acetylcholinesterase-blocking effects are prohibited in warfare by international law (Geneva Protocol. https://www.un.org/disarmament/wmd/bio/1925-geneva-protocol/). Monitoring PO analogs with acetylcholinesterase-blocking effects and their degradation products in water and soil can provide clues to unlawful production sites and the possible use of POs in warfare. Attempts to analyze POs by derivatization have had a low ability to detect them. A neurophysiological detector (NPD)-high-performance liquid chromatography (HPLC) system was developed for specific detection with high detection ability. The first official presentation of our NPD was at the 3<sup>rd</sup> International Symposium on Separation in BioSciences SBS 2003: A 100 Years of Chromatography, May 13-18, 2003, in Moscow, Russia. The NPD in connection to HPLC was developed 14 years before the presentation at the SBS in 2003. Initially, NPD combined with an HPLC system was developed for intelligence services and only for use in monitoring and espionage against the unlawful production of neuroparalytic agents, as explained in this article. NPD combined with an HPLC system was developed in Umeå, Sweden, in 1987-89;the protocol was further developed in Statens Plantevern Institutt, Ås, Norway, in 1990-92. NPD may have great utility during the current period of active warfare in Europe. The initial challenge was detecting unlawful production and use of PO compounds and their metabolites that can potentially block acetylcholinesterase. The sensor in NPD can detect and monitor substances such as tabun, soman, and modern PO poisons used in military applications. This article describes the history of the development of NPD and its aim as a sensitive sensor in detecting PO substances with acetylcholinesterase-blocking effects.展开更多
文摘Herein,a colorimetric chemosensor(probe 7)was designed and synthesized for sensing diethyl cyanophosphonate(DCNP),a Tabun nerve agent simulant.The sensitive,selective,fast response and photostable probe 7 in dimethylsulfoxide(DMSO)responded to DCNP at low concentrations with visual naked eye color change from pink to pale yellow and displayed unresponsive behaviour against various interferences except for copper ions,which can be differentiated by adding ethylene diamine where the yellow color persisted in the case of DCNP but turned to pink in the case of copper ions.Additionally,the yellow aqueous solution of probe 7 at pH 10 and 12 turned colorless against the response of DCNP.The limit of detection(LOD)of probe 7 was found to be 50 nmol/L in an aqueous basic solution at pH 10 for the detection of DCNP.The formation of the cyclized product was elucidated by 1H and 31P NMR nuclear magnetic resonance(1H NMR,31P NMR),Fourier transform infrared spectroscopy(FTIR),and high-resolution mass spectrometry(HRMS)analysis.The filter paper and silica plate loaded with probe 7 were fabricated for real-time and on-site detection of DCNP.
文摘The main aim for discovery and development of the neurophysiological detector was detection of the production’ seats and criminal use of poisons in warfare. Phosphor-organic (PO) substances with acetylcholinesterase-blocking effects are prohibited in warfare by international law (Geneva Protocol. https://www.un.org/disarmament/wmd/bio/1925-geneva-protocol/). Monitoring PO analogs with acetylcholinesterase-blocking effects and their degradation products in water and soil can provide clues to unlawful production sites and the possible use of POs in warfare. Attempts to analyze POs by derivatization have had a low ability to detect them. A neurophysiological detector (NPD)-high-performance liquid chromatography (HPLC) system was developed for specific detection with high detection ability. The first official presentation of our NPD was at the 3<sup>rd</sup> International Symposium on Separation in BioSciences SBS 2003: A 100 Years of Chromatography, May 13-18, 2003, in Moscow, Russia. The NPD in connection to HPLC was developed 14 years before the presentation at the SBS in 2003. Initially, NPD combined with an HPLC system was developed for intelligence services and only for use in monitoring and espionage against the unlawful production of neuroparalytic agents, as explained in this article. NPD combined with an HPLC system was developed in Umeå, Sweden, in 1987-89;the protocol was further developed in Statens Plantevern Institutt, Ås, Norway, in 1990-92. NPD may have great utility during the current period of active warfare in Europe. The initial challenge was detecting unlawful production and use of PO compounds and their metabolites that can potentially block acetylcholinesterase. The sensor in NPD can detect and monitor substances such as tabun, soman, and modern PO poisons used in military applications. This article describes the history of the development of NPD and its aim as a sensitive sensor in detecting PO substances with acetylcholinesterase-blocking effects.
