目的:观察温通针法对慢性非细菌性前列腺炎大鼠血清TNF-α、IL-1β含量的影响,探讨温通针法治疗慢性非细菌性前列腺炎的作用机理。方法:将60只Wistar雄性大鼠,采用25%消痔灵注射液0.2 m L(每单侧叶注射0.1 m L)注入前列腺背侧叶,造成慢...目的:观察温通针法对慢性非细菌性前列腺炎大鼠血清TNF-α、IL-1β含量的影响,探讨温通针法治疗慢性非细菌性前列腺炎的作用机理。方法:将60只Wistar雄性大鼠,采用25%消痔灵注射液0.2 m L(每单侧叶注射0.1 m L)注入前列腺背侧叶,造成慢性非细菌性前列腺炎大鼠动物模型。造模后随机分成模型组、药物组、平补平泻组和温通针法4组,每组15只。模型组不予任何刺激,药物组给予前列康灌胃,平补平泻组和温通针法组均取关元、曲骨、行间,平补平泻组行平补平泻手法,温通针法组行温通针法,三治疗组均是每日治疗1次,共治15次,最后观察各组大鼠血清中TNF-α、IL-1β的水平。结果:温通针法组较平补平泻组和药物组能明显降低大鼠血清中的TNF-α(P均<0.01);温通针法组较药物组能明显降低大鼠血清中的IL-1β(P<0.01),与平补平泻组相比无统计学意义。结论:温通针法治疗慢性非细菌性前列腺炎疗效显著,效果独特,其机理与降低TNF-α、IL-1β含量,减轻前列腺组织内炎细胞浸润和炎症反应,具有抗炎和调节局部免疫反应的作用有关。展开更多
Sulfur mustard (SM) is an alkalizing chemical which has been used mostly as a weapon all over the world. Sulfur mustard can cause damages to many organs, especially the skin, respiratory system and the eyes. Generally...Sulfur mustard (SM) is an alkalizing chemical which has been used mostly as a weapon all over the world. Sulfur mustard can cause damages to many organs, especially the skin, respiratory system and the eyes. Generally, many complications of mustard gas result from its alkalizing potency and reaction with cellular components like DNA, RNA, proteins and lipid membranes. The damages caused by SM will lead to many complications which persist during the lifespan of exposed subjects. Pro-inflammatory cytokines including especially TNF-α and IL-1β can cause systemic inflammatory reactions and vast changes like altered cell signaling, migration, cytokine production changes and fever. This study was designed to analyze cytokine levels in mustard-gas-exposed people’s serum in the war between Iraq and Iran, who had the chronic dry-eye symptoms compared to the normal group, 30 years after exposure. In this study, 25 veterans who were exposed to mustard gas were compared to 25 healthy people as control group. The veterans with concurrent involvement of eye, lung, and skin were selected. We used ELISA method to assess the levels of TNF-α and IL-1β in serum of people in both groups. All the results analyzed with T-test in SPSS 17 statistical software. The mean levels of TNF-α and IL-1β in serum of chemical exposed veterans were 52.3 ± 1.4 pg/ml and 3.43 ± 0.3 pg/ml while in the control group were 19.5±1.3 pg/ml and 2.25 ± 0.2 pg/ml, respectively. In the control group, the serum pro-inflammatory cytokine levels were significantly lower than the exposed group (P < 0.05). This study showed that there is a meaningful difference between TNF-α and IL-1β serum levels in the SM exposed group compared to the control group. There are some differences between the present study and others. However, studies on local inflammatory changes in these patients are also limited and need more reviews.展开更多
文摘目的:观察温通针法对慢性非细菌性前列腺炎大鼠血清TNF-α、IL-1β含量的影响,探讨温通针法治疗慢性非细菌性前列腺炎的作用机理。方法:将60只Wistar雄性大鼠,采用25%消痔灵注射液0.2 m L(每单侧叶注射0.1 m L)注入前列腺背侧叶,造成慢性非细菌性前列腺炎大鼠动物模型。造模后随机分成模型组、药物组、平补平泻组和温通针法4组,每组15只。模型组不予任何刺激,药物组给予前列康灌胃,平补平泻组和温通针法组均取关元、曲骨、行间,平补平泻组行平补平泻手法,温通针法组行温通针法,三治疗组均是每日治疗1次,共治15次,最后观察各组大鼠血清中TNF-α、IL-1β的水平。结果:温通针法组较平补平泻组和药物组能明显降低大鼠血清中的TNF-α(P均<0.01);温通针法组较药物组能明显降低大鼠血清中的IL-1β(P<0.01),与平补平泻组相比无统计学意义。结论:温通针法治疗慢性非细菌性前列腺炎疗效显著,效果独特,其机理与降低TNF-α、IL-1β含量,减轻前列腺组织内炎细胞浸润和炎症反应,具有抗炎和调节局部免疫反应的作用有关。
文摘Sulfur mustard (SM) is an alkalizing chemical which has been used mostly as a weapon all over the world. Sulfur mustard can cause damages to many organs, especially the skin, respiratory system and the eyes. Generally, many complications of mustard gas result from its alkalizing potency and reaction with cellular components like DNA, RNA, proteins and lipid membranes. The damages caused by SM will lead to many complications which persist during the lifespan of exposed subjects. Pro-inflammatory cytokines including especially TNF-α and IL-1β can cause systemic inflammatory reactions and vast changes like altered cell signaling, migration, cytokine production changes and fever. This study was designed to analyze cytokine levels in mustard-gas-exposed people’s serum in the war between Iraq and Iran, who had the chronic dry-eye symptoms compared to the normal group, 30 years after exposure. In this study, 25 veterans who were exposed to mustard gas were compared to 25 healthy people as control group. The veterans with concurrent involvement of eye, lung, and skin were selected. We used ELISA method to assess the levels of TNF-α and IL-1β in serum of people in both groups. All the results analyzed with T-test in SPSS 17 statistical software. The mean levels of TNF-α and IL-1β in serum of chemical exposed veterans were 52.3 ± 1.4 pg/ml and 3.43 ± 0.3 pg/ml while in the control group were 19.5±1.3 pg/ml and 2.25 ± 0.2 pg/ml, respectively. In the control group, the serum pro-inflammatory cytokine levels were significantly lower than the exposed group (P < 0.05). This study showed that there is a meaningful difference between TNF-α and IL-1β serum levels in the SM exposed group compared to the control group. There are some differences between the present study and others. However, studies on local inflammatory changes in these patients are also limited and need more reviews.
