To clarify the influences of the tip clearance flows on the unsteady cavitating flow, the three-dimensional unsteady cavitating flows through both the two-dimensional cascades and the three-dimensional inducer with an...To clarify the influences of the tip clearance flows on the unsteady cavitating flow, the three-dimensional unsteady cavitating flows through both the two-dimensional cascades and the three-dimensional inducer with and without tip clearance are performed numerically. The governing equations for the compressible fluid flow with the DES turbulence model are employed with the assumption of the isentropic process of liquid phase. The evolution of cavities is represented as the source/sink of vapor phase. The basic equations in the curve linear coordinate are solved by the finite difference method. As the results of the three-dimensional cavitating flows through the two-dimensional cascades, the tip clearance flows from the pressure side to the suction side of the blade produces the tip vortex cavitation, which affects the sheet cavitation on the leading edge of the next blade and enhances the blockage effect near the casing than the flows without tip clearance. On the other hand, in the case of the three-dimensional inducer, the large backflow cavitation is observed around the inlet of the inducer, where the cavities are developed on the casing by the tip clearance flows. The large pressure gradient between the non-cavitating pressure side and the cavitating suction side enhances the tip clearance flows. The calculation considering the tip clearance reproduces the developed cavitation region similar to that of experimental visualizations. Additionally, the backflow cavitation rotates with the speed slower than the rotation speed of the inducer. Then, the rotation of backflow cavitation causes the periodic fluctuation of the outlet pressure greater than that of the inlet pressure.展开更多
Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers o...Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen.specific members of calrnodulin （CAM）, CaM-like （CML）, calcium-dependent protein kinase （CDPK） and calcineurin B-like protein （CBL） families were tagged with green fluorescence protein （GFP） and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane （PM）, and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.展开更多
This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee...This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.展开更多
The inception of the tip vortex cavitation（TVC） is a very important problem in cavitation researches. The study of the mechanism of the TVC inception is not only conducive to its prediction, but also helps to suppre...The inception of the tip vortex cavitation（TVC） is a very important problem in cavitation researches. The study of the mechanism of the TVC inception is not only conducive to its prediction, but also helps to suppress or suspend the occurrence of cavitation. In this paper, the research progresses on the TVC inception including theoretical, experimental and numerical studies mainly in the last two decades are reviewed. It is shown that the TVC inception is affected by complicated factors, such as the water quality, the average pressure and the fluctuating pressure. In the scaling law for the determination of the TVC inception, all these factors are considered. To precisely describe the scaling law, more investigations are needed to understand the effects of the water quality and the fluctuating pressure.展开更多
Cutting tests were done using a test bed designed to measure pick cutting forces when cutting coal and rock.The test equipment has a drum with two starting helical vanes.Cutting forces on a pick were measured as a fun...Cutting tests were done using a test bed designed to measure pick cutting forces when cutting coal and rock.The test equipment has a drum with two starting helical vanes.Cutting forces on a pick were measured as a function of coal compressive strength,pick carbide tip diameter and the cutting depth per drum revolution.The results show that the cutting force is linearly related to the compressive strength.The relationship between the cutting force and both the carbide tip diameter and the cutting depth are exponential.Fluctuation in the cutting force does not increase with coal compressive strength but it has a linear relationship to tip diameter.A plot of cutting force fluctuations versus the cutting depth follows a sigmoidal curve.Based on the analysis of these test results a theoretical basis is supplied for design and effective use of shearer drums.展开更多
In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with...In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with section NACA 662-415 was adopted as test model. High-speed video（HSV） camera was used to visualize the trajectory of tip vortex core and the form of tip vortex cavitation（TVC） in different cavitation situations. Laser Doppler velocimetry（LDV） was employed to measure the tip vortex flow field in some typical sections along the vortex trajectory with the case of cavitation free. Stereo particle image velocimetry（SPIV） system was used to measure the velocity and vorticity distributions with and without cavitation. Series measurement results such as velocity and vorticity distributions, the trajectory of tip vortex core, the vortex core radius, cavity size and cavitation inception number were obtained. The results demonstrated that the minimum pressure coefficient in the vortex core obtained by flow field measurement was quite coincident with the tip vortex cavitation inception number obtained under the condition of high incoming velocity and low air content. And TVC would decrease the vortex strength comparing with the case without cavitation.展开更多
Axial flow pump is a kind of typical pumps with rotor-stator interaction, thus the measurement of the flow field between impeller and guide vane would facilitate the study of the internal rotor-stator interaction mech...Axial flow pump is a kind of typical pumps with rotor-stator interaction, thus the measurement of the flow field between impeller and guide vane would facilitate the study of the internal rotor-stator interaction mechanism. Through a structural modification of a traditional axial flow pump, the requirements of particle image velocimetry（PIV） measurement are met. Under the condition of opt.0.8Q, the axial vortex is identified between impeller hub and guide vane hub, which is developed into the main flow and to affect the movement when the relative positions of impeller and guide vane at different flow rates are the same. Besides, the development and the dissipation of the tip leakage and the passage vortex in impeller passages are mainly responsible for the difference of the flow field close to the outer rim. As the flow rate decreases, the distribution of the meridional velocities at the impeller outlet becomes more non-uniform and the radial velocity component keeps increasing. The PIV measurement results under the condition of opt.1.0Q indicate that the flow separation and the trailing vortex at the trailing edge of a blade are likely to result in a velocity sudden change in this area, which would dramatically destroy the continuity of the flow field. Moreover, the radial direction of the flow between impeller and guide vane on the measurement plane does not always point from hub to rim. For a certain position, the direction is just from rim to hub, as is affected by the location of the intersection line of the shooting section and the impeller blade on the impeller as well as the angle between the intersection line and the rotating shaft.展开更多
Simulations of tip vortex wetted flows and cavitating flows are carried out by using a RANS model. Two types of turbule- nce models, with and without the Boussinesq turbulent-viscosity hypothesis, are adopted in compa...Simulations of tip vortex wetted flows and cavitating flows are carried out by using a RANS model. Two types of turbule- nce models, with and without the Boussinesq turbulent-viscosity hypothesis, are adopted in comparing with experimental results regarding the vorticity, the strain rate and the Reynolds shear stress distributions in the vortex region. The numerical results imply that the spatial phase shift between the mean strain rate and the Reynolds stresses can be accurately modeled by the nonlinear κ-ε turbulence model, the tip vortex cavitation region can only be predicted using the nonlinear κ-ε turbulence model. The mecha- nism of the over-dissipation due to the turbulence model is analyzed in terms of the turbulence production, which is one of the dominant source terms in the transport equations of energy.展开更多
The loss in efficiency due to shroud leakage or tip clearance flow accounts for a substantial part of the overall losses in turbomachinery. It is important to identify the leakage loss characteristics in order to opti...The loss in efficiency due to shroud leakage or tip clearance flow accounts for a substantial part of the overall losses in turbomachinery. It is important to identify the leakage loss characteristics in order to optimize turbomachinery. At present, little information is available in the open literature concerning the effect of honeycomb seals on the loss characteristics in shroud cavities of an axial turbine, despite of the widespread use of the honeycomb seals. Therefore, interaction between rotor labyrinth seal leakage flow with and without honeycomb facings and main flow is investigated to provide the loss characteristics of the mixing process of the re-entering leakage flow into the main flow. The effects of honeycomb seals on the flow in shroud cavities and interaction with the main flow are analyzed. An additional study on the impact of subtle shroud cavity exit geometry is also presented. The investigation results indicate that the honeycomb seal affects the over tip leakage flow and reduces mixing losses when compared to the solid labyrinth seal. The leakage flow interactions with the main flow have considerably changed the flow fields in the endwall regions. The proposed research reveals the effects of honeycomb seals on the loss characteristics in shroud cavities and the impact of subtle shroud cavity exit geometry, and it is helpful for the design optimization of turbomachinery.展开更多
文摘筛选出了适合于桃品种‘北农早艳’茎尖(0.2 mm 和0.5 mm)培养的培养基为:G培养基附加 IBA 0.25 mg/L+BA 0.5 mg/L+GA_3 1.0 mg/L+LH 500.0 mg/L。茎尖用聚氨酯海绵作支撑物的液体培养比琼脂培养成活率高2～5倍,且生长迅速。获得了0.2 mm 长茎尖的生根植株。
文摘To clarify the influences of the tip clearance flows on the unsteady cavitating flow, the three-dimensional unsteady cavitating flows through both the two-dimensional cascades and the three-dimensional inducer with and without tip clearance are performed numerically. The governing equations for the compressible fluid flow with the DES turbulence model are employed with the assumption of the isentropic process of liquid phase. The evolution of cavities is represented as the source/sink of vapor phase. The basic equations in the curve linear coordinate are solved by the finite difference method. As the results of the three-dimensional cavitating flows through the two-dimensional cascades, the tip clearance flows from the pressure side to the suction side of the blade produces the tip vortex cavitation, which affects the sheet cavitation on the leading edge of the next blade and enhances the blockage effect near the casing than the flows without tip clearance. On the other hand, in the case of the three-dimensional inducer, the large backflow cavitation is observed around the inlet of the inducer, where the cavities are developed on the casing by the tip clearance flows. The large pressure gradient between the non-cavitating pressure side and the cavitating suction side enhances the tip clearance flows. The calculation considering the tip clearance reproduces the developed cavitation region similar to that of experimental visualizations. Additionally, the backflow cavitation rotates with the speed slower than the rotation speed of the inducer. Then, the rotation of backflow cavitation causes the periodic fluctuation of the outlet pressure greater than that of the inlet pressure.
基金Supported by the State Key Basic Research and Development Plan of Chin(2007CB108704)
文摘Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen.specific members of calrnodulin （CAM）, CaM-like （CML）, calcium-dependent protein kinase （CDPK） and calcineurin B-like protein （CBL） families were tagged with green fluorescence protein （GFP） and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane （PM）, and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.
文摘This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.
基金Project supported by the State Key Program of National Natural Science of China(Grant No.11332009)the National Natural Science Foundation of China(Grant No.11272284)
文摘The inception of the tip vortex cavitation（TVC） is a very important problem in cavitation researches. The study of the mechanism of the TVC inception is not only conducive to its prediction, but also helps to suppress or suspend the occurrence of cavitation. In this paper, the research progresses on the TVC inception including theoretical, experimental and numerical studies mainly in the last two decades are reviewed. It is shown that the TVC inception is affected by complicated factors, such as the water quality, the average pressure and the fluctuating pressure. In the scaling law for the determination of the TVC inception, all these factors are considered. To precisely describe the scaling law, more investigations are needed to understand the effects of the water quality and the fluctuating pressure.
基金Projects 2008AA062202 supported by the Hi-tech Research and Development Program of ChinaCX08B_041Z by the Innovation Foundation of Jiangsu Graduate Students
文摘Cutting tests were done using a test bed designed to measure pick cutting forces when cutting coal and rock.The test equipment has a drum with two starting helical vanes.Cutting forces on a pick were measured as a function of coal compressive strength,pick carbide tip diameter and the cutting depth per drum revolution.The results show that the cutting force is linearly related to the compressive strength.The relationship between the cutting force and both the carbide tip diameter and the cutting depth are exponential.Fluctuation in the cutting force does not increase with coal compressive strength but it has a linear relationship to tip diameter.A plot of cutting force fluctuations versus the cutting depth follows a sigmoidal curve.Based on the analysis of these test results a theoretical basis is supplied for design and effective use of shearer drums.
基金Project supported by the Key Project of National Natural Science Foundation of China(Grant No.11332009)
文摘In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with section NACA 662-415 was adopted as test model. High-speed video（HSV） camera was used to visualize the trajectory of tip vortex core and the form of tip vortex cavitation（TVC） in different cavitation situations. Laser Doppler velocimetry（LDV） was employed to measure the tip vortex flow field in some typical sections along the vortex trajectory with the case of cavitation free. Stereo particle image velocimetry（SPIV） system was used to measure the velocity and vorticity distributions with and without cavitation. Series measurement results such as velocity and vorticity distributions, the trajectory of tip vortex core, the vortex core radius, cavity size and cavitation inception number were obtained. The results demonstrated that the minimum pressure coefficient in the vortex core obtained by flow field measurement was quite coincident with the tip vortex cavitation inception number obtained under the condition of high incoming velocity and low air content. And TVC would decrease the vortex strength comparing with the case without cavitation.
基金Project supported by the National Twelfth Five-year Supporting Plan of China(Grant No.2011BAF14B01)the Priority Academic Program Development of Jiangsu Higher Education InstitutionsGraduate innovation program of Jiangsu Province(Grant No.CXLX12_0643)
文摘Axial flow pump is a kind of typical pumps with rotor-stator interaction, thus the measurement of the flow field between impeller and guide vane would facilitate the study of the internal rotor-stator interaction mechanism. Through a structural modification of a traditional axial flow pump, the requirements of particle image velocimetry（PIV） measurement are met. Under the condition of opt.0.8Q, the axial vortex is identified between impeller hub and guide vane hub, which is developed into the main flow and to affect the movement when the relative positions of impeller and guide vane at different flow rates are the same. Besides, the development and the dissipation of the tip leakage and the passage vortex in impeller passages are mainly responsible for the difference of the flow field close to the outer rim. As the flow rate decreases, the distribution of the meridional velocities at the impeller outlet becomes more non-uniform and the radial velocity component keeps increasing. The PIV measurement results under the condition of opt.1.0Q indicate that the flow separation and the trailing vortex at the trailing edge of a blade are likely to result in a velocity sudden change in this area, which would dramatically destroy the continuity of the flow field. Moreover, the radial direction of the flow between impeller and guide vane on the measurement plane does not always point from hub to rim. For a certain position, the direction is just from rim to hub, as is affected by the location of the intersection line of the shooting section and the impeller blade on the impeller as well as the angle between the intersection line and the rotating shaft.
基金supported by the National Natural Science Foundation of China(Grant No.11332009)the Key Doctoral Program Foundation of Shanghai Municipality(Grant No.B206)
文摘Simulations of tip vortex wetted flows and cavitating flows are carried out by using a RANS model. Two types of turbule- nce models, with and without the Boussinesq turbulent-viscosity hypothesis, are adopted in comparing with experimental results regarding the vorticity, the strain rate and the Reynolds shear stress distributions in the vortex region. The numerical results imply that the spatial phase shift between the mean strain rate and the Reynolds stresses can be accurately modeled by the nonlinear κ-ε turbulence model, the tip vortex cavitation region can only be predicted using the nonlinear κ-ε turbulence model. The mecha- nism of the over-dissipation due to the turbulence model is analyzed in terms of the turbulence production, which is one of the dominant source terms in the transport equations of energy.
基金supported by National Natural Science Foundation of China (Grant No. 50776021)Doctoral Fund of Ministry of Education of China (Grant No. 20092304110004)
文摘The loss in efficiency due to shroud leakage or tip clearance flow accounts for a substantial part of the overall losses in turbomachinery. It is important to identify the leakage loss characteristics in order to optimize turbomachinery. At present, little information is available in the open literature concerning the effect of honeycomb seals on the loss characteristics in shroud cavities of an axial turbine, despite of the widespread use of the honeycomb seals. Therefore, interaction between rotor labyrinth seal leakage flow with and without honeycomb facings and main flow is investigated to provide the loss characteristics of the mixing process of the re-entering leakage flow into the main flow. The effects of honeycomb seals on the flow in shroud cavities and interaction with the main flow are analyzed. An additional study on the impact of subtle shroud cavity exit geometry is also presented. The investigation results indicate that the honeycomb seal affects the over tip leakage flow and reduces mixing losses when compared to the solid labyrinth seal. The leakage flow interactions with the main flow have considerably changed the flow fields in the endwall regions. The proposed research reveals the effects of honeycomb seals on the loss characteristics in shroud cavities and the impact of subtle shroud cavity exit geometry, and it is helpful for the design optimization of turbomachinery.
