目的:探索慢性胃病、胃癌中 TGFα和 cyclin E 表达与脾证分型的关系。方法:在慢性胃病和胃癌患者中,选取中医脾气(阳)虚、脾阴虚、脾胃湿热、寒湿困脾证患者共143例,进行胃粘膜组织的 TGFα和 cyclin E 免疫组化染色,对各证型的 TGFα...目的:探索慢性胃病、胃癌中 TGFα和 cyclin E 表达与脾证分型的关系。方法:在慢性胃病和胃癌患者中,选取中医脾气(阳)虚、脾阴虚、脾胃湿热、寒湿困脾证患者共143例,进行胃粘膜组织的 TGFα和 cyclin E 免疫组化染色,对各证型的 TGFα和 cyclin E 表达进行比较,并分析它们表达的相关性。结果:TGFα和 cyclin E 在上述四种脾证分型中的表达有显著性差异,TGFα表达的阳性率分别为15.8%、33.3%、41.2%和19.0%,cyclin E 则分别为7.9%、27.3% 31.4% 5和14.3%。TGFα和 cyclin E 表达存在显著的相关关系。结论:在慢性胃病、胃癌中,TGFα和 cyclin E 表达在不同中医证型中有明显差异,在脾胃湿热和脾阴虚证型中较高,在寒湿困脾和脾气(阳)虚证型中较低。TGFα和cyclin E 表达存在相关关系。展开更多
Fat is an indispensable nutrient and basic metabolite for sustaining life,and milk is particularly rich in fatty acids,including a variety of saturated and unsaturated fatty acids.MicroRNA(miRNA)and mRNA play an impor...Fat is an indispensable nutrient and basic metabolite for sustaining life,and milk is particularly rich in fatty acids,including a variety of saturated and unsaturated fatty acids.MicroRNA(miRNA)and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue.It has been shown that lipid metabolism has a complex transcriptional regulation,but the mechanism by which milk fat synthesis is regulated through miRNA–mRNA interactions is poorly understood.In this study,we performed transcriptome sequencing with bovine mammary gland tissue in the late lactation(270 and 315 days after parturition)to identify the key gene that regulating milk fat metabolism.A total of 1207 differentially coexpressed genes were selected,828 upregulated genes and 379 downregulated genes were identified.The transforming growth factor alpha(TGFA)gene was selected as the target gene,and luciferase reporter assay,Western blotting and q RT-PCR were used for further study.The results demonstrated that miR-140 was an upstream regulator of TGFA,and miR-140 could inhibit(P<0.01)unsaturated fatty acid and triglyceride(TAGs)production in bovine mammary epithelial cells(BMECs).In contrast,TGFA promoted(P<0.01)unsaturated fatty acid and TAG production.Rescue experiments further indicated the mi R-140/TGFA regulatory mechanism.Taken together,these results suggest that the mi R-140/TGFA pathway can inhibit(P<0.01)milk fat metabolism and improve milk quality by genetic means.展开更多
文摘目的:探索慢性胃病、胃癌中 TGFα和 cyclin E 表达与脾证分型的关系。方法:在慢性胃病和胃癌患者中,选取中医脾气(阳)虚、脾阴虚、脾胃湿热、寒湿困脾证患者共143例,进行胃粘膜组织的 TGFα和 cyclin E 免疫组化染色,对各证型的 TGFα和 cyclin E 表达进行比较,并分析它们表达的相关性。结果:TGFα和 cyclin E 在上述四种脾证分型中的表达有显著性差异,TGFα表达的阳性率分别为15.8%、33.3%、41.2%和19.0%,cyclin E 则分别为7.9%、27.3% 31.4% 5和14.3%。TGFα和 cyclin E 表达存在显著的相关关系。结论:在慢性胃病、胃癌中,TGFα和 cyclin E 表达在不同中医证型中有明显差异,在脾胃湿热和脾阴虚证型中较高,在寒湿困脾和脾气(阳)虚证型中较低。TGFα和cyclin E 表达存在相关关系。
基金supported by the National Natural Science Foundation of China (31802035, 31872324 and 31601915)
文摘Fat is an indispensable nutrient and basic metabolite for sustaining life,and milk is particularly rich in fatty acids,including a variety of saturated and unsaturated fatty acids.MicroRNA(miRNA)and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue.It has been shown that lipid metabolism has a complex transcriptional regulation,but the mechanism by which milk fat synthesis is regulated through miRNA–mRNA interactions is poorly understood.In this study,we performed transcriptome sequencing with bovine mammary gland tissue in the late lactation(270 and 315 days after parturition)to identify the key gene that regulating milk fat metabolism.A total of 1207 differentially coexpressed genes were selected,828 upregulated genes and 379 downregulated genes were identified.The transforming growth factor alpha(TGFA)gene was selected as the target gene,and luciferase reporter assay,Western blotting and q RT-PCR were used for further study.The results demonstrated that miR-140 was an upstream regulator of TGFA,and miR-140 could inhibit(P<0.01)unsaturated fatty acid and triglyceride(TAGs)production in bovine mammary epithelial cells(BMECs).In contrast,TGFA promoted(P<0.01)unsaturated fatty acid and TAG production.Rescue experiments further indicated the mi R-140/TGFA regulatory mechanism.Taken together,these results suggest that the mi R-140/TGFA pathway can inhibit(P<0.01)milk fat metabolism and improve milk quality by genetic means.
