Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery effic...Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.展开更多
目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常...目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常规护理,观察组在常规护理基础上实施早期预见性护理干预。对比两组患者的住院时间、护理满意度、并发症发生率以及护理前后焦虑自评量表(SAS)和抑郁自评量表(SDS)评分情况。结果:观察组患者住院时间与并发症发生率低于对照组,满意度高于对照组,差异有统计学意义(P<0.05);护理后,两组患者SAS和SDS评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05);两组患者护理前Harris评分与VAS评分对比,差异无统计学意义(P>0.05),两组患者护理后Harris评分均升高,且观察组高于对照组,差异有统计学意义(P<0.05);两组护理后V A S评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论:对股骨TFNA手术患者在术中应用早期预见性护理,能够降低患者术后并发症发生率,减少住院时间,减轻患者焦虑、抑郁等不良情绪,减轻患者术后疼痛程度,提升髋关节功能,提高患者护理满意度,值得临床应用。展开更多
基金supported by National Key R&D Program of China (No. 2019YFA0110600)National Natural Science Foundation of China (No. 81970916)+2 种基金Sichuan Province Youth Science and Technology Innovation Team (No. 2022JDTD0021)Research Funding from West China School/Hospital of Stomatology Sichuan University (No. RCDWJS2021-20)China Postdoctoral Science Foundation (No. 2022TQ0381)。
文摘Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.
文摘目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常规护理,观察组在常规护理基础上实施早期预见性护理干预。对比两组患者的住院时间、护理满意度、并发症发生率以及护理前后焦虑自评量表(SAS)和抑郁自评量表(SDS)评分情况。结果:观察组患者住院时间与并发症发生率低于对照组,满意度高于对照组,差异有统计学意义(P<0.05);护理后,两组患者SAS和SDS评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05);两组患者护理前Harris评分与VAS评分对比,差异无统计学意义(P>0.05),两组患者护理后Harris评分均升高,且观察组高于对照组,差异有统计学意义(P<0.05);两组护理后V A S评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论:对股骨TFNA手术患者在术中应用早期预见性护理,能够降低患者术后并发症发生率,减少住院时间,减轻患者焦虑、抑郁等不良情绪,减轻患者术后疼痛程度,提升髋关节功能,提高患者护理满意度,值得临床应用。
