应用MIKE 11和MIKE 21分别构建北京市大兴区天堂河下游地区一维和二维水动力学模型,采用DHI MIKE FLOOD耦合模拟了不同暴雨重现期下该地区的淹没特性.为分析该地区暴雨洪涝灾害的危险性,研究模拟了不同情景下各骨干渠道考虑限制下泄流...应用MIKE 11和MIKE 21分别构建北京市大兴区天堂河下游地区一维和二维水动力学模型,采用DHI MIKE FLOOD耦合模拟了不同暴雨重现期下该地区的淹没特性.为分析该地区暴雨洪涝灾害的危险性,研究模拟了不同情景下各骨干渠道考虑限制下泄流量的排水状况,整个研究区淹没范围随时间的演变趋势和空间上的分布状况.研究结果表明,整个研究区现状防洪能力不足20a一遇设计标准,且研究区局部区域最大淹没深度超过2m,最大积水时间超过8h,是防洪排涝的重点区域.该结果可为下一步暴雨风险评估,滞洪区的选址和闸坝调度方案的制定提供参考依据.展开更多
Background Fibroblast growth factor 21 （FGF21） is a new member of FGF super family that is an important endogenous regulator for systemic glucose and lipid metabolism. This study aimed to explore whether FGF21 reduc...Background Fibroblast growth factor 21 （FGF21） is a new member of FGF super family that is an important endogenous regulator for systemic glucose and lipid metabolism. This study aimed to explore whether FGF21 reduces atherosclerotic injury and prevents endothelial dysfunction as an independent protection factor.Methods The present study was designed to investigate the changes of FGF21 levels induced by oxidized-low density lipoprotein （ox-LDL）, and the changes of apoptosis affected by regulating FGF21 expression. The FGF21 mRNA levels of cultured cardiac microvascular endothelial cells （CMECs） were determined by real time-PCR and the protein concentration in culture media was detected by enzyme-linked immunosorbent assay. We analyzed the different expression levels of untreated controls and CMFCs incubated with ox-LDL, and the changes of CMECs apoptosis initiated by the enhancement or suppression of FGF21 levels.Results The secretion levels of FGF21 mRNA and protein were significantly upregulated in CMECs incubated with ox-LDL. Furthermore, FGF21 levels increased by 200 μmol/L bezafibrate could reduce CMECs apoptosis, and inhibit FGF21 expression by shRNA induced apoptosis （P ＜0.05）.Conclusions FGF21 may be a signal of injured target tissue, and may play physiological roles in improving the endothelial function at an early stage of atherosclerosis and in stopping the development of coronary heart disease.展开更多
Background:Epidermal growth factor receptor(EGFR) mutations,including a known exon 19 deletion(19 del) and exon 21 L858 R point mutation(L858R mutation),are strong predictors of the response to EGFR tyrosine kinase in...Background:Epidermal growth factor receptor(EGFR) mutations,including a known exon 19 deletion(19 del) and exon 21 L858 R point mutation(L858R mutation),are strong predictors of the response to EGFR tyrosine kinase inhibitor(EGFR-TKI) treatment in lung adenocarcinoma.However,whether patients carrying EGFR 19 del and L858 R mutations exhibit different responsiveness to EGFR-TKls and what are the potential mechanism for this difference remain controversial.This study aimed to investigate the clinical outcomes of EGFR-TKI treatment in patients with EGFR 19 del and L858 R mutations and explore the genetic heterogeneity of tumors with the two mutation subtypes.Methods:Of 1127 patients with advanced lung adenocarcinoma harboring EGFR 19 del or L858 R mutations,532 received EGFR-TKI treatment and were included in this study.EGFR 19 del and L858 R mutations were detected by using denaturing high-performance liquid chromatography(DHPLC).T790 M mutation,which is a common resistant mutation on exon 20 of EGFR,was detected by amplification refractory mutation system(ARMS).Next-generation sequencing(NGS) was used to explore the genetic heterogeneity of tumors with EGFR 19 del and L858 R mutations.Results:Of the 532 patients,319(60.0%) had EGFR 19 del,and 213(40.0%) had L858 R mutations.The patients with EGFR 19 del presented a significantly higher overall response rate(ORR) for EGFR-TKI treatment(55.2%vs.43.7%,P = 0.017) and had a longer progression-free survival(PFS) after first-line EGFR-TKI treatment(14.4 vs.11.4 months,P = 0.034) compared with those with L858 R mutations.However,no statistically significant difference in overall survival(OS) was observed between the two groups of patients.T790 M mutation status was analyzed in 88 patients before EGFR-TKI treatment and 134 after EGFR-TKI treatment,and there was no significant difference in the co-existence of T790 M mutation with EGFR 19 del and L858 R mutations before EGFR-TKI treatment(5.6%vs.8.8%,P = 0.554)or after treatment(24.4%vs.35.4%,P = 0.176).In addition,24 patie展开更多
Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal i...Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal imaging system was selected to measure the infrared radiation temperature on body surface of eight points in four meridians in 74 patients with hyperplasia of mammary glands and 63 healthy people. Results： The infrared radiation temperatures on body surface of right Youmen （KI 21） in cases with hyperplasia of mammary glands were significantly higher than the healthy people （P=-0.009）, and the infrared radiation temperatures on left Youmen （KI 21） and other points had no significant difference with healthy people （P〉0.05）. Conclusion： The infrared radiation temperatures on body surface of right Youmen （KI 21） have significant differences with healthy people. This might occur because of the pathogenesis of hyperplasia of mammary glands and of the specificity of the point Youmen （KI 21）.展开更多
The morbidity and mortality of cardiovascular diseases(CVDs)are increasing worldwide and seriously threaten human life and health.Fibroblast growth factor 21(FGF21),a metabolic regulator,regulates glucose and lipid me...The morbidity and mortality of cardiovascular diseases(CVDs)are increasing worldwide and seriously threaten human life and health.Fibroblast growth factor 21(FGF21),a metabolic regulator,regulates glucose and lipid metabolism and may exert beneficial effects on the cardiovascular system.In recent years,FGF21 has been found to act directly on the cardiovascular system and may be used as an early biomarker of CVDs.The present review highlights the recent progress in understanding the relationship between FGF21 and CVDs including coronary heart disease,myocardial ischemia,cardiomyopathy,and heart failure and also explores the related mechanism of the cardioprotective effect of FGF21.FGF21 plays an important role in the prediction,treatment,and improvement of prognosis in CVDs.This cardioprotective effect of FGF21 may be achieved by preventing endothelial dysfunction and lipid accumulating,inhibiting cardiomyocyte apoptosis and regulating the associated oxidative stress,inflammation and autophagy.In conclusion,FGF21 is a promising target for the treatment of CVDs,however,its clinical application requires further clarification of the precise role of FGF21 in CVDs.展开更多
文摘应用MIKE 11和MIKE 21分别构建北京市大兴区天堂河下游地区一维和二维水动力学模型,采用DHI MIKE FLOOD耦合模拟了不同暴雨重现期下该地区的淹没特性.为分析该地区暴雨洪涝灾害的危险性,研究模拟了不同情景下各骨干渠道考虑限制下泄流量的排水状况,整个研究区淹没范围随时间的演变趋势和空间上的分布状况.研究结果表明,整个研究区现状防洪能力不足20a一遇设计标准,且研究区局部区域最大淹没深度超过2m,最大积水时间超过8h,是防洪排涝的重点区域.该结果可为下一步暴雨风险评估,滞洪区的选址和闸坝调度方案的制定提供参考依据.
基金This study was supported by a grant from National Natural Science Foundation of China （No. 81070227）.
文摘Background Fibroblast growth factor 21 （FGF21） is a new member of FGF super family that is an important endogenous regulator for systemic glucose and lipid metabolism. This study aimed to explore whether FGF21 reduces atherosclerotic injury and prevents endothelial dysfunction as an independent protection factor.Methods The present study was designed to investigate the changes of FGF21 levels induced by oxidized-low density lipoprotein （ox-LDL）, and the changes of apoptosis affected by regulating FGF21 expression. The FGF21 mRNA levels of cultured cardiac microvascular endothelial cells （CMECs） were determined by real time-PCR and the protein concentration in culture media was detected by enzyme-linked immunosorbent assay. We analyzed the different expression levels of untreated controls and CMFCs incubated with ox-LDL, and the changes of CMECs apoptosis initiated by the enhancement or suppression of FGF21 levels.Results The secretion levels of FGF21 mRNA and protein were significantly upregulated in CMECs incubated with ox-LDL. Furthermore, FGF21 levels increased by 200 μmol/L bezafibrate could reduce CMECs apoptosis, and inhibit FGF21 expression by shRNA induced apoptosis （P ＜0.05）.Conclusions FGF21 may be a signal of injured target tissue, and may play physiological roles in improving the endothelial function at an early stage of atherosclerosis and in stopping the development of coronary heart disease.
基金supported by grants from the National Natural Sciences Foundation Key Program(No.81330062)Education Ministry Innovative Research Team Program(No.IRT13003)+1 种基金Peking University-Tsinghua University Joint Center for Life Sciences Clinical Investigator,National High Technology Research and Development Program 863(No.SS2015AA020403)Beijing Technology Project(No.Z141100000214013)
文摘Background:Epidermal growth factor receptor(EGFR) mutations,including a known exon 19 deletion(19 del) and exon 21 L858 R point mutation(L858R mutation),are strong predictors of the response to EGFR tyrosine kinase inhibitor(EGFR-TKI) treatment in lung adenocarcinoma.However,whether patients carrying EGFR 19 del and L858 R mutations exhibit different responsiveness to EGFR-TKls and what are the potential mechanism for this difference remain controversial.This study aimed to investigate the clinical outcomes of EGFR-TKI treatment in patients with EGFR 19 del and L858 R mutations and explore the genetic heterogeneity of tumors with the two mutation subtypes.Methods:Of 1127 patients with advanced lung adenocarcinoma harboring EGFR 19 del or L858 R mutations,532 received EGFR-TKI treatment and were included in this study.EGFR 19 del and L858 R mutations were detected by using denaturing high-performance liquid chromatography(DHPLC).T790 M mutation,which is a common resistant mutation on exon 20 of EGFR,was detected by amplification refractory mutation system(ARMS).Next-generation sequencing(NGS) was used to explore the genetic heterogeneity of tumors with EGFR 19 del and L858 R mutations.Results:Of the 532 patients,319(60.0%) had EGFR 19 del,and 213(40.0%) had L858 R mutations.The patients with EGFR 19 del presented a significantly higher overall response rate(ORR) for EGFR-TKI treatment(55.2%vs.43.7%,P = 0.017) and had a longer progression-free survival(PFS) after first-line EGFR-TKI treatment(14.4 vs.11.4 months,P = 0.034) compared with those with L858 R mutations.However,no statistically significant difference in overall survival(OS) was observed between the two groups of patients.T790 M mutation status was analyzed in 88 patients before EGFR-TKI treatment and 134 after EGFR-TKI treatment,and there was no significant difference in the co-existence of T790 M mutation with EGFR 19 del and L858 R mutations before EGFR-TKI treatment(5.6%vs.8.8%,P = 0.554)or after treatment(24.4%vs.35.4%,P = 0.176).In addition,24 patie
基金State 973 plan (2005CB523306)National Natural Science Foundation (30572306)+1 种基金Shanghai Municipal Education Commission Project (07CZ034)State Administration of Traditional Chinese Medicine and Shanghai Leading Academic Discipline Project (T0304,T0302)
文摘Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal imaging system was selected to measure the infrared radiation temperature on body surface of eight points in four meridians in 74 patients with hyperplasia of mammary glands and 63 healthy people. Results： The infrared radiation temperatures on body surface of right Youmen （KI 21） in cases with hyperplasia of mammary glands were significantly higher than the healthy people （P=-0.009）, and the infrared radiation temperatures on left Youmen （KI 21） and other points had no significant difference with healthy people （P〉0.05）. Conclusion： The infrared radiation temperatures on body surface of right Youmen （KI 21） have significant differences with healthy people. This might occur because of the pathogenesis of hyperplasia of mammary glands and of the specificity of the point Youmen （KI 21）.
基金the General Program of National Natural Science Foundation of China(NSFC)(81870598)Excellent Young ScholarsofNSFC(82022012)Two Hundred Program from Shanghai Jiao Tong University School of Medicine(20191830)to HL.
文摘The morbidity and mortality of cardiovascular diseases(CVDs)are increasing worldwide and seriously threaten human life and health.Fibroblast growth factor 21(FGF21),a metabolic regulator,regulates glucose and lipid metabolism and may exert beneficial effects on the cardiovascular system.In recent years,FGF21 has been found to act directly on the cardiovascular system and may be used as an early biomarker of CVDs.The present review highlights the recent progress in understanding the relationship between FGF21 and CVDs including coronary heart disease,myocardial ischemia,cardiomyopathy,and heart failure and also explores the related mechanism of the cardioprotective effect of FGF21.FGF21 plays an important role in the prediction,treatment,and improvement of prognosis in CVDs.This cardioprotective effect of FGF21 may be achieved by preventing endothelial dysfunction and lipid accumulating,inhibiting cardiomyocyte apoptosis and regulating the associated oxidative stress,inflammation and autophagy.In conclusion,FGF21 is a promising target for the treatment of CVDs,however,its clinical application requires further clarification of the precise role of FGF21 in CVDs.
