目的分析湖北地区汉族人群T细胞免疫球蛋白域黏蛋白域蛋白4（T cells immunoglobulin domin and mucin domain protein 4，TIM4）基因8570G〉A、11515C〉A单核苷酸多态性，探讨其与变应性哮喘易感性之间的关系。方法采用聚合酶链反应和...目的分析湖北地区汉族人群T细胞免疫球蛋白域黏蛋白域蛋白4（T cells immunoglobulin domin and mucin domain protein 4，TIM4）基因8570G〉A、11515C〉A单核苷酸多态性，探讨其与变应性哮喘易感性之间的关系。方法采用聚合酶链反应和限制性片段长度多态性对145例变应性哮喘患者和130名健康对照T／M4基因8570G〉A和11515C〉A多态性进行分析，计算基因型和等位基因频率。结果湖北地区健康人群TIM48570G〉AGG、GA和AA基因型频率分别是0．985、0．015和0，而哮喘患者其频率分别为0．931、0．069、0，基因型和等位基因频率在病例组与对照组间差异有统计学意义（P=0．030，P=0．032）；未检测到T／M4基因11515C〉A的多态性。结论湖北汉族人群T／M4基因8570G〉A存在单核苷酸多态性变异，可能与湖北地区汉族变应性哮喘易感性有关。展开更多
The relationship between T cell immunoglobulin domain and mucin domain protein 3（Tim-3）/Galectin（Gal）-9 pathway and recurrent spontaneous abortion（RSA） was studied. Thirty-one pregnant women with RSA and 27 norm...The relationship between T cell immunoglobulin domain and mucin domain protein 3（Tim-3）/Galectin（Gal）-9 pathway and recurrent spontaneous abortion（RSA） was studied. Thirty-one pregnant women with RSA and 27 normal early gravidas were investigated to detect the levels of Tim-3 and Gal-9 in villi and deciduas by Western blotting. Meanwhile, the concentration of interleukin（IL）-4 and IL-12 in peripheral blood plasma was determined by ELISA in 25 healthy fertile non-pregnant controls, the normal early gravidas and pregnant women with RSA mentioned above, respectively. It was found that the relative expression levels of Tim-3 and Gal-9 in villi and deciduas were significantly increased in pregnant women with RSA as compared with those in the normal early gravidas. The concentration of IL-4 in peripheral blood plasma of pregnant women with RSA was lower than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）, but that of IL-2 in pregnant women with RSA was significantly higher than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）. It was suggested that the overexpression of Tim-3/Gal-9 pathway may be related to the pathogenesis of RSA.展开更多
To investigate the distribution characteristics and linkage disequilibrium of T cell immunoglobulin domain and mucin domain protein 4 （TIM4） promoter polymorphisms in asthma patients of Chinese Han population, the p...To investigate the distribution characteristics and linkage disequilibrium of T cell immunoglobulin domain and mucin domain protein 4 （TIM4） promoter polymorphisms in asthma patients of Chinese Han population, the promoter region of TIM4 was re-sequenced by PCR-sequencing, and linkage disequilibrium was analyzed by SHEsis software. Four single nucleotide polymor- phisms （SNPs） in the promoter region of TIM4 were detected, including two new SNPs （at positions -1609, -153） and two reported SNPs （rs6874202, rs6882076）. The frequency distribution of rs6882076 was different among different races （P〈0.05）. In addition, linkage disequilibrium among the SNPs of the promoter region of TIM4 was found and GGTG was the predominant haplotype. There were four SNPs in the promoter region of TIM4 in asthma patients of Chinese Han population, which were in linkage disequilibrium.展开更多
文摘目的分析湖北地区汉族人群T细胞免疫球蛋白域黏蛋白域蛋白4（T cells immunoglobulin domin and mucin domain protein 4，TIM4）基因8570G〉A、11515C〉A单核苷酸多态性，探讨其与变应性哮喘易感性之间的关系。方法采用聚合酶链反应和限制性片段长度多态性对145例变应性哮喘患者和130名健康对照T／M4基因8570G〉A和11515C〉A多态性进行分析，计算基因型和等位基因频率。结果湖北地区健康人群TIM48570G〉AGG、GA和AA基因型频率分别是0．985、0．015和0，而哮喘患者其频率分别为0．931、0．069、0，基因型和等位基因频率在病例组与对照组间差异有统计学意义（P=0．030，P=0．032）；未检测到T／M4基因11515C〉A的多态性。结论湖北汉族人群T／M4基因8570G〉A存在单核苷酸多态性变异，可能与湖北地区汉族变应性哮喘易感性有关。
基金supported by grants from the National Science Foundation of China(Nos.30973205 and 81172464)the National "Twelfth-Five Year" Research and Development Program of China(No.2014BAI05B05)
文摘The relationship between T cell immunoglobulin domain and mucin domain protein 3（Tim-3）/Galectin（Gal）-9 pathway and recurrent spontaneous abortion（RSA） was studied. Thirty-one pregnant women with RSA and 27 normal early gravidas were investigated to detect the levels of Tim-3 and Gal-9 in villi and deciduas by Western blotting. Meanwhile, the concentration of interleukin（IL）-4 and IL-12 in peripheral blood plasma was determined by ELISA in 25 healthy fertile non-pregnant controls, the normal early gravidas and pregnant women with RSA mentioned above, respectively. It was found that the relative expression levels of Tim-3 and Gal-9 in villi and deciduas were significantly increased in pregnant women with RSA as compared with those in the normal early gravidas. The concentration of IL-4 in peripheral blood plasma of pregnant women with RSA was lower than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）, but that of IL-2 in pregnant women with RSA was significantly higher than that of the normal early gravidas（P〈0.05） and healthy fertile non-pregnant controls（P〈0.05）. It was suggested that the overexpression of Tim-3/Gal-9 pathway may be related to the pathogenesis of RSA.
基金the National Natural Sciences Foundation of China (No. 30672008)
文摘To investigate the distribution characteristics and linkage disequilibrium of T cell immunoglobulin domain and mucin domain protein 4 （TIM4） promoter polymorphisms in asthma patients of Chinese Han population, the promoter region of TIM4 was re-sequenced by PCR-sequencing, and linkage disequilibrium was analyzed by SHEsis software. Four single nucleotide polymor- phisms （SNPs） in the promoter region of TIM4 were detected, including two new SNPs （at positions -1609, -153） and two reported SNPs （rs6874202, rs6882076）. The frequency distribution of rs6882076 was different among different races （P〈0.05）. In addition, linkage disequilibrium among the SNPs of the promoter region of TIM4 was found and GGTG was the predominant haplotype. There were four SNPs in the promoter region of TIM4 in asthma patients of Chinese Han population, which were in linkage disequilibrium.
