OBJECTIVES: To investigate the expressions of E-eadherin and alpha-catenin in pancreatic carcinoma and their relationship with biological behaviors, and clarify the mechanism of invasion and metastasis of pancreatic c...OBJECTIVES: To investigate the expressions of E-eadherin and alpha-catenin in pancreatic carcinoma and their relationship with biological behaviors, and clarify the mechanism of invasion and metastasis of pancreatic cancer. METHODS: The expressions of E-eadherin and alpha-catenin was examined in 47 patients with infiltrative ductal adenocarcinoma of the pancreas and 12 specimens of normal pancreatic tissues by immunohistochemical technique (PicTure^(TM) two-step method). Proliferation cell nuclear antigen (PCNA) was tested as an index of the proliferation degree of pancreatic cancer cells. RESULTS: The immunoreactivity of E-cadherin and alpha-catenin was expressed by normal ductal and acinar cells with strong membranous staining at the intercellular border in 12 specimens of normal pancreatic tissues. The abnormal rate of E-cadherin expression in pancreatic cancer was 53.2% (25/47), and it was significantly related to differentiation, high proliferation degree and lymph node and liver metastases (P<0.O1, 0.05, 0.05 and O.01, respectively). 61.7% patients with pancreatic cancer (29/47) showed abnormal expression of alpha-catenin. There was a good correlation among alpha-catenin expression, histological grade, and lymph node and liver metastases (P<0.05, 0.05 and 0.01, respectively). No significant association was found among abnormal expressions of E-cadherin and alpha-catenin, tumor size, invasion, and 1-year survival rate of patients ( P>0.05, all). There was a positive relationship between the expressions of E-cadherin and alpha-catenin in the 47 patients with pancreatic cancer (P<O.O1, r=0.88). CONCLUSIONS: Pancreatic cancer likely occurs in case of the inactivation of E-cadherin and alpha-catenin genes and abnormal expression of proteins, which significantly correlate with tumorigenesis, proliferation, differentiation, and lymph node or liver metastasis of pancreatic cancer.展开更多
Toxicity of Bacillus thuringiensis delta endotoxin is interrelated to larval midgut membrane receptor and its binding affinity and numbers.Binding to a specific receptor is an important determinant with respect to dif...Toxicity of Bacillus thuringiensis delta endotoxin is interrelated to larval midgut membrane receptor and its binding affinity and numbers.Binding to a specific receptor is an important determinant with respect to differences in insecticidal spectrum of Bacillus thuringiensis delta endotoxin.Membrane receptor has different binding sites which can interact specially with different binding determinants on the toxin molecules.Meanwhile,endotoxin might interact with more than one receptor in BBMV.The interaction of toxin and membrane receptor shows both the complexity and diversity,but not only recognizing one by one simply.Insect resistance to Bt is also related to the midgut membrane receptor in binding sites,affinity and numbers.It was shown that midgut membrane receptors are brush border membrane vesicles on the membrane of midgut epithelial cells,they belong to aminopeptidase family or cadherin family.Aminopeptidase acts as receptor just on its presence,but not its enzymatic activity.展开更多
基金Supported by National Natural Science Foundation of China,No.30770991 and No.30800511Esophageal Carcinoma Innovative Research Program of Jiangsu Provincial Hospitals
文摘AIM: To investigate whether hypoxia inducible factor (HIF)-1α modulates vasculogenic mimicry (VM) by upregulating VE-cadherin expression in esophageal squamous cell carcinoma (ESCC).
文摘OBJECTIVES: To investigate the expressions of E-eadherin and alpha-catenin in pancreatic carcinoma and their relationship with biological behaviors, and clarify the mechanism of invasion and metastasis of pancreatic cancer. METHODS: The expressions of E-eadherin and alpha-catenin was examined in 47 patients with infiltrative ductal adenocarcinoma of the pancreas and 12 specimens of normal pancreatic tissues by immunohistochemical technique (PicTure^(TM) two-step method). Proliferation cell nuclear antigen (PCNA) was tested as an index of the proliferation degree of pancreatic cancer cells. RESULTS: The immunoreactivity of E-cadherin and alpha-catenin was expressed by normal ductal and acinar cells with strong membranous staining at the intercellular border in 12 specimens of normal pancreatic tissues. The abnormal rate of E-cadherin expression in pancreatic cancer was 53.2% (25/47), and it was significantly related to differentiation, high proliferation degree and lymph node and liver metastases (P<0.O1, 0.05, 0.05 and O.01, respectively). 61.7% patients with pancreatic cancer (29/47) showed abnormal expression of alpha-catenin. There was a good correlation among alpha-catenin expression, histological grade, and lymph node and liver metastases (P<0.05, 0.05 and 0.01, respectively). No significant association was found among abnormal expressions of E-cadherin and alpha-catenin, tumor size, invasion, and 1-year survival rate of patients ( P>0.05, all). There was a positive relationship between the expressions of E-cadherin and alpha-catenin in the 47 patients with pancreatic cancer (P<O.O1, r=0.88). CONCLUSIONS: Pancreatic cancer likely occurs in case of the inactivation of E-cadherin and alpha-catenin genes and abnormal expression of proteins, which significantly correlate with tumorigenesis, proliferation, differentiation, and lymph node or liver metastasis of pancreatic cancer.
文摘Toxicity of Bacillus thuringiensis delta endotoxin is interrelated to larval midgut membrane receptor and its binding affinity and numbers.Binding to a specific receptor is an important determinant with respect to differences in insecticidal spectrum of Bacillus thuringiensis delta endotoxin.Membrane receptor has different binding sites which can interact specially with different binding determinants on the toxin molecules.Meanwhile,endotoxin might interact with more than one receptor in BBMV.The interaction of toxin and membrane receptor shows both the complexity and diversity,but not only recognizing one by one simply.Insect resistance to Bt is also related to the midgut membrane receptor in binding sites,affinity and numbers.It was shown that midgut membrane receptors are brush border membrane vesicles on the membrane of midgut epithelial cells,they belong to aminopeptidase family or cadherin family.Aminopeptidase acts as receptor just on its presence,but not its enzymatic activity.
