In the theory of traditional Chinese medicine, aging is mainly thought renal deficiency caused renal failure, mainly involving decline of kidney-Yang and deficiency of kidney-essence. Huanshaodan, a Chinese traditiona...In the theory of traditional Chinese medicine, aging is mainly thought renal deficiency caused renal failure, mainly involving decline of kidney-Yang and deficiency of kidney-essence. Huanshaodan, a Chinese traditional preparation for kidney-replenishing essence, was used to be the preparation for reinforcing renal deficiency and preventing aging for aged people. OBJECTIVE： To observe the effects of Huanshaodan on swimming durance and the abilities of catalase （CAT） in serum and monoamine oxidase-B （MAO-B） in brain tissue as well as in vitro anti-oxidative ability of aging mouse. DESIGN： A controlled animal experiment. SETTING： College of Basic Medicine, Hunan University of Traditional Chinese Medicine. MATERIALS： Fifty-four healthy NIH mice, aged 18 months old, of either gender, weighing （48.9±5.4） g, and one SD male rat, aged 16 months old, weighing 51.7 g, were provided by Animal Experimental Center, Hunan University of Traditional Chinese Medicine. Thirty NIH mice were randomly chosen for swimming test, and divided into experimental group and control group, with 15 in each; The other 24 NIH mice were used for enzyme activity assay, and also divided into experimental group and control group, with 12 in each. SD rat was used for in vitro anti-oxidative ability test, Huanshaodan water decoction was composed of Cheqianzi, Wuweizi, Huaishan, Danggui, Huangbai, Shudi, Baizhi, Niuxi, Baishen, Tusizi, Buguzhi, Roucongrong and Heshouwu 13 Chinese herbs. METHODS： This study was carried out in the Second Laboratory, Department of Biochemistry, Hunan University of Traditional Chinese Medicine in June 2006. Swimming and enzyme activity assay： Mice in the two experimental groups were intragastrically administrated with l0 μL/g Huanshaodan water decoction. Mice in the two control groups were intragastrically administrated with the same amount of normal saline. All the mice were intragastrically administrated for 5 days, and they were free to access to medicine in the other 2 days in a week. Each mo展开更多
文摘In the theory of traditional Chinese medicine, aging is mainly thought renal deficiency caused renal failure, mainly involving decline of kidney-Yang and deficiency of kidney-essence. Huanshaodan, a Chinese traditional preparation for kidney-replenishing essence, was used to be the preparation for reinforcing renal deficiency and preventing aging for aged people. OBJECTIVE： To observe the effects of Huanshaodan on swimming durance and the abilities of catalase （CAT） in serum and monoamine oxidase-B （MAO-B） in brain tissue as well as in vitro anti-oxidative ability of aging mouse. DESIGN： A controlled animal experiment. SETTING： College of Basic Medicine, Hunan University of Traditional Chinese Medicine. MATERIALS： Fifty-four healthy NIH mice, aged 18 months old, of either gender, weighing （48.9±5.4） g, and one SD male rat, aged 16 months old, weighing 51.7 g, were provided by Animal Experimental Center, Hunan University of Traditional Chinese Medicine. Thirty NIH mice were randomly chosen for swimming test, and divided into experimental group and control group, with 15 in each; The other 24 NIH mice were used for enzyme activity assay, and also divided into experimental group and control group, with 12 in each. SD rat was used for in vitro anti-oxidative ability test, Huanshaodan water decoction was composed of Cheqianzi, Wuweizi, Huaishan, Danggui, Huangbai, Shudi, Baizhi, Niuxi, Baishen, Tusizi, Buguzhi, Roucongrong and Heshouwu 13 Chinese herbs. METHODS： This study was carried out in the Second Laboratory, Department of Biochemistry, Hunan University of Traditional Chinese Medicine in June 2006. Swimming and enzyme activity assay： Mice in the two experimental groups were intragastrically administrated with l0 μL/g Huanshaodan water decoction. Mice in the two control groups were intragastrically administrated with the same amount of normal saline. All the mice were intragastrically administrated for 5 days, and they were free to access to medicine in the other 2 days in a week. Each mo
