人工控制条件下生长的小麦、玉米分别培养在含0、0.5、1.0和2.0mM SO_4^(-2)4个硫营养水平的Hoagland 培养液中,对2—6周龄的植株进行了 RuBPCase、PEPCase、NR 的活性,叶片可溶性蛋白质含量,叶绿素 a 和 b 含量以及植株最终叶面积和总...人工控制条件下生长的小麦、玉米分别培养在含0、0.5、1.0和2.0mM SO_4^(-2)4个硫营养水平的Hoagland 培养液中,对2—6周龄的植株进行了 RuBPCase、PEPCase、NR 的活性,叶片可溶性蛋白质含量,叶绿素 a 和 b 含量以及植株最终叶面积和总干重的测定。结果指出,硫营养不足影响了作物的碳、氮代谢,随着供硫量的增加,上述各参数值均得到了有利于植物生长发育的改善,说明一定量的硫营养是氮代谢所必需的,硫对碳代谢的影响恐怕是与其对蛋白质和酶的影响相联系的。展开更多
Sulfide-containing waste streams are generated by a number of industries. It is emitted into the environment as dis- solved sulfide (S2- and HS-) in wastewaters and as H2S in waste gases. Due to its corrosive nature, ...Sulfide-containing waste streams are generated by a number of industries. It is emitted into the environment as dis- solved sulfide (S2- and HS-) in wastewaters and as H2S in waste gases. Due to its corrosive nature, biological hydrogen sulfide removal processes are being investigated to overcome the chemical and disposal costs associated with existing chemically based removal processes. The nitrogen and sulfur metabolism interacts at various levels of the wastewater treatment process. Hence, the sulfur cycle offers possibilities to integrate nitrogen removal in the treatment process, which needs to be further optimized by appropriate design of the reactor configuration, optimization of performance parameters, retention of biomass and optimization of biomass growth. The present paper reviews the biotechnological advances to remove sulfides from various environments.展开更多
Plant take up the essential nutrient sulfur as sulfate from the soil, reduce it, and assimilate into bioorganic compounds, with cysteine being the first product. Both sulfate uptake and assimilation are highly regulat...Plant take up the essential nutrient sulfur as sulfate from the soil, reduce it, and assimilate into bioorganic compounds, with cysteine being the first product. Both sulfate uptake and assimilation are highly regulated by the demand for the reduced sulfur, by availability of nutrients, and by environmental conditions. In the last decade, great prog- ress has been achieved in dissecting the regulation of sulfur metabolism. Sulfate uptake and reduction of activated sulfate, adenosine 5＇-phosphosulfate （APS）, to sulfite by APS reductase appear to be the key regulatory steps. Here, we review the current knowledge on regulation of these processes, with special attention given to similarities and differences.展开更多
The effects of surfactant Tween-80 on the growth, sulfur oxidation, and expression of selected typical sulfur metabolism relevant genes of Acidithiobacillus ferrooxidans ATCC 23270 were investigated. The results showe...The effects of surfactant Tween-80 on the growth, sulfur oxidation, and expression of selected typical sulfur metabolism relevant genes of Acidithiobacillus ferrooxidans ATCC 23270 were investigated. The results showed that in the presence of 10-2 g/L Tween-80, the growth of A. ferrooxidans and its metabolism on the insoluble substrate S0 and CuFeS2 were promoted. After 24 d of bioleaching, the copper extraction yield of chalcopyrite at 10-2 g/L Tween-80 increased by 16% compared with the bioleaching experiment without Tween-80. FT-IR spectra analysis revealed that the result was probably caused by the extracellular polymeric substances whose composition could be changed by the surfactant addition. RT-qPCR was used to analyze the differential expressions of 17 selected sulfur metabolism relevant genes in response to the addition of Tween-80. Down-regulation of the extracellular protein genes indicated the influence of Tween-80 on bacteria-sulfur adsorption. Variation of the expression level of the enzymes provided a supplement to sulfur metabolism investigation.展开更多
文摘人工控制条件下生长的小麦、玉米分别培养在含0、0.5、1.0和2.0mM SO_4^(-2)4个硫营养水平的Hoagland 培养液中,对2—6周龄的植株进行了 RuBPCase、PEPCase、NR 的活性,叶片可溶性蛋白质含量,叶绿素 a 和 b 含量以及植株最终叶面积和总干重的测定。结果指出,硫营养不足影响了作物的碳、氮代谢,随着供硫量的增加,上述各参数值均得到了有利于植物生长发育的改善,说明一定量的硫营养是氮代谢所必需的,硫对碳代谢的影响恐怕是与其对蛋白质和酶的影响相联系的。
基金Project supported by the National Natural Science Foundation ofChina (No. 30070017)the Science and Technology Foundationfor Key Project of Zhejiang Province (No. 2003C13005), China
文摘Sulfide-containing waste streams are generated by a number of industries. It is emitted into the environment as dis- solved sulfide (S2- and HS-) in wastewaters and as H2S in waste gases. Due to its corrosive nature, biological hydrogen sulfide removal processes are being investigated to overcome the chemical and disposal costs associated with existing chemically based removal processes. The nitrogen and sulfur metabolism interacts at various levels of the wastewater treatment process. Hence, the sulfur cycle offers possibilities to integrate nitrogen removal in the treatment process, which needs to be further optimized by appropriate design of the reactor configuration, optimization of performance parameters, retention of biomass and optimization of biomass growth. The present paper reviews the biotechnological advances to remove sulfides from various environments.
文摘Plant take up the essential nutrient sulfur as sulfate from the soil, reduce it, and assimilate into bioorganic compounds, with cysteine being the first product. Both sulfate uptake and assimilation are highly regulated by the demand for the reduced sulfur, by availability of nutrients, and by environmental conditions. In the last decade, great prog- ress has been achieved in dissecting the regulation of sulfur metabolism. Sulfate uptake and reduction of activated sulfate, adenosine 5＇-phosphosulfate （APS）, to sulfite by APS reductase appear to be the key regulatory steps. Here, we review the current knowledge on regulation of these processes, with special attention given to similarities and differences.
基金Projects (50974140, 51274257) supported by the National Natural Science Foundation of ChinaProject (20090162110054) supported by the PhD Programs Foundation of Ministry of Education of China
文摘The effects of surfactant Tween-80 on the growth, sulfur oxidation, and expression of selected typical sulfur metabolism relevant genes of Acidithiobacillus ferrooxidans ATCC 23270 were investigated. The results showed that in the presence of 10-2 g/L Tween-80, the growth of A. ferrooxidans and its metabolism on the insoluble substrate S0 and CuFeS2 were promoted. After 24 d of bioleaching, the copper extraction yield of chalcopyrite at 10-2 g/L Tween-80 increased by 16% compared with the bioleaching experiment without Tween-80. FT-IR spectra analysis revealed that the result was probably caused by the extracellular polymeric substances whose composition could be changed by the surfactant addition. RT-qPCR was used to analyze the differential expressions of 17 selected sulfur metabolism relevant genes in response to the addition of Tween-80. Down-regulation of the extracellular protein genes indicated the influence of Tween-80 on bacteria-sulfur adsorption. Variation of the expression level of the enzymes provided a supplement to sulfur metabolism investigation.
