Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model...Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.展开更多
目的:观察椎针通督循经疗法对脑中风患者肢体痉挛状态的影响。方法:将84例脑中风患者随机分为两组,分别为治疗组和对照组,各42例。对照组采用传统针刺,治疗组采用椎针通督循经疗法,两组患者在治疗期间均进行基本的药物治疗和康复训练,...目的:观察椎针通督循经疗法对脑中风患者肢体痉挛状态的影响。方法:将84例脑中风患者随机分为两组,分别为治疗组和对照组,各42例。对照组采用传统针刺,治疗组采用椎针通督循经疗法,两组患者在治疗期间均进行基本的药物治疗和康复训练,疗程结束后采用Fugl-meyer运动功能评分(FMA),改良的Ashworth评分和改良Barthel指数对运动功能、痉挛程度和日常生活能力进行比较,采用神经功能缺损评分(neural function defect Score,NFDS)对神经功能缺损程度变化情况做比较,并比较两组的临床疗效。结果:两组患者治疗前后比较,FMA评分、Ashworth评分、改良Barthel指数、NFDS评分均有显著改善(P<0.05);与对照组比较,治疗组以上各指标的改善幅度较显著,差异有统计学意义(P<0.05);对照组有效率为78.57%,治疗组有效率为95.24%,两组比较差异有统计学意义(P<0.05)。结论:椎针通督循经疗法可以显著改善脑中风患者的运动功能、肢体痉挛程度和日常生活能力。展开更多
基金supported by the National Natural Science Foundation of China,No.14202225,&.81574042the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,No.2000202a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,No.JDZX2015314
文摘Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
文摘目的:观察椎针通督循经疗法对脑中风患者肢体痉挛状态的影响。方法:将84例脑中风患者随机分为两组,分别为治疗组和对照组,各42例。对照组采用传统针刺,治疗组采用椎针通督循经疗法,两组患者在治疗期间均进行基本的药物治疗和康复训练,疗程结束后采用Fugl-meyer运动功能评分(FMA),改良的Ashworth评分和改良Barthel指数对运动功能、痉挛程度和日常生活能力进行比较,采用神经功能缺损评分(neural function defect Score,NFDS)对神经功能缺损程度变化情况做比较,并比较两组的临床疗效。结果:两组患者治疗前后比较,FMA评分、Ashworth评分、改良Barthel指数、NFDS评分均有显著改善(P<0.05);与对照组比较,治疗组以上各指标的改善幅度较显著,差异有统计学意义(P<0.05);对照组有效率为78.57%,治疗组有效率为95.24%,两组比较差异有统计学意义(P<0.05)。结论:椎针通督循经疗法可以显著改善脑中风患者的运动功能、肢体痉挛程度和日常生活能力。
