MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attra...MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.展开更多
With the accelerated development of globalization,the importance of English as an international language is self-evident.However,traditional English teaching often focuses on grammar and vocabulary,while neglecting pr...With the accelerated development of globalization,the importance of English as an international language is self-evident.However,traditional English teaching often focuses on grammar and vocabulary,while neglecting practical applications.In order to cultivate students who can use English fluently and confidently,we need to conduct in-depth discussions on English teaching strategies and methods.In terms of speaking,teachers can arrange classroom discussions,role-playing,and other activities to encourage students to speak English boldly.Through continuous practice,students can gradually overcome shyness and nervousness,improve their fluency and confidence in oral expression.This teaching method can not only help students better understand the English language,but also broaden their horizons and improve their cross-cultural communication skills.This article will illustrate the necessity of practical English teaching through examples and case studies,and explore effective teaching strategies and methods.展开更多
Graphene(GR),a single‐layer carbon sheet with a hexagonal packed lattice structure,has displayed attractive potential and demonstrably become the research focus in artificial photocatalysis due to its enchanting prop...Graphene(GR),a single‐layer carbon sheet with a hexagonal packed lattice structure,has displayed attractive potential and demonstrably become the research focus in artificial photocatalysis due to its enchanting properties in enhancing light absorption,electron transfer dynamics,and surface reactions.Currently,numerous efforts have shown that the properties of GR,which are closely correlated to the photocatalytic performance of GR‐based composites are significantly affected by the synthesis methods.Herein,we first introduce the optimization strategies of GR‐based hybrids and then elaborate the synthesis of GR‐based composite photocatalysts oriented by manifold roles of GR in photoredox catalysis,containing photoelectron mediator and acceptor,improving adsorption capacity,regulating light absorption range and intensity,as well as macromolecular photosensitizer.Beyond that,a brief outlook on the challenges in this burgeoning research field and potential evolution strategies for enhancing the photoactivity of GR‐based hybrids is presented and we anticipate that this review could provide some enlightenments for the rational construction and application of multifunctional GR‐based composite photocatalysts.展开更多
基金financial support from the National Natural Science Foundation of China(Grant 81573389)the National Key R&D Program of China(2017YFC0908600)
文摘MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.
文摘With the accelerated development of globalization,the importance of English as an international language is self-evident.However,traditional English teaching often focuses on grammar and vocabulary,while neglecting practical applications.In order to cultivate students who can use English fluently and confidently,we need to conduct in-depth discussions on English teaching strategies and methods.In terms of speaking,teachers can arrange classroom discussions,role-playing,and other activities to encourage students to speak English boldly.Through continuous practice,students can gradually overcome shyness and nervousness,improve their fluency and confidence in oral expression.This teaching method can not only help students better understand the English language,but also broaden their horizons and improve their cross-cultural communication skills.This article will illustrate the necessity of practical English teaching through examples and case studies,and explore effective teaching strategies and methods.
文摘随着智能电网的发展,分布式电源(distributed generation,DG)广泛接入电力系统。然而以风能和太阳能为代表的单个DG对大电网来说视为不可见,在参与市场交易时存在诸多不利因素。考虑多能源资源的影响,建立了由DG、负荷以及储能组成的虚拟发电厂(virtual power plant,VPP)模型,同时将多个VPP进行互联,在满足各自VPP内部供需平衡的基础上,让盈余电量在VPP集群间进行共享,实现多阶段市场交易。提出的交易方法以VPP内部和网间的交易收益最大化为优化目标函数,以尽可能消纳可再生能源为目的,同时计入需求响应(demand response,DR)策略,得到网内用户科学用电计划、网间合理报价结果,为实现多个VPP健康灵活交易提供参考。
基金国家"863"计划项目"抗旱节水品种筛选与高效用水种植技术"(2011AA100501)国际农业合作挑战计划项目"Wheat breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance in China"(G7010.02.01)+2 种基金山西省科技攻关和科技产业化项目"小麦抗旱优质高产新材料创制与强筋新品种选育"(20090311001-3)"优质小麦新品种及栽培技术示范推广"(2013071001-1)农业科技成果转化资金项目"强筋抗旱丰产国审小麦新品种运旱20410示范推广"(2012G B2A 300057)
文摘Graphene(GR),a single‐layer carbon sheet with a hexagonal packed lattice structure,has displayed attractive potential and demonstrably become the research focus in artificial photocatalysis due to its enchanting properties in enhancing light absorption,electron transfer dynamics,and surface reactions.Currently,numerous efforts have shown that the properties of GR,which are closely correlated to the photocatalytic performance of GR‐based composites are significantly affected by the synthesis methods.Herein,we first introduce the optimization strategies of GR‐based hybrids and then elaborate the synthesis of GR‐based composite photocatalysts oriented by manifold roles of GR in photoredox catalysis,containing photoelectron mediator and acceptor,improving adsorption capacity,regulating light absorption range and intensity,as well as macromolecular photosensitizer.Beyond that,a brief outlook on the challenges in this burgeoning research field and potential evolution strategies for enhancing the photoactivity of GR‐based hybrids is presented and we anticipate that this review could provide some enlightenments for the rational construction and application of multifunctional GR‐based composite photocatalysts.
