An 11 year study was conducted to reevaluate the species of stipitate hydnums from the southern United States especially the Great Smoky Mountains and surrounding geography.The genera evaluated included Bankera herein...An 11 year study was conducted to reevaluate the species of stipitate hydnums from the southern United States especially the Great Smoky Mountains and surrounding geography.The genera evaluated included Bankera herein reduced to synonym with Phellodon,Hydnellum,Sarcodon,and Phellodon.Basidiomata of many stipitate hydnum species from this region were abundant but nitrogen depositions during the last 20 years have impacted their occurrences.Therefore it became critical to do a definitive study of these fungi using current taxonomic tools.Many species within Hydnellum and Phellodon have a indeterminate type growth often dependant on environmental conditions making discrimination between closely related species difficult without supportive molecular sequence data.Once sequence data(ITS)was obtained a phylogenetic analyzes were conducted and the sequences were compared to those in GenBank with particular attention to European data.A total of 41 distinct taxa were determined and many of those were confirmed based on morphological data.A specific new combination from Bankera fuligineoalbum to Phellodon fuligineoalbus is made herein.Futhermore,Phellodon brunneoolivaceus is described as new based on morphological and sequence data.展开更多
The development of economically feasible bio-based process requires efficient cell factories capable of producing the desired product at high titer under high-cell-density fermentation.Herein we present a combinatoria...The development of economically feasible bio-based process requires efficient cell factories capable of producing the desired product at high titer under high-cell-density fermentation.Herein we present a combinatorial approach based on systems metabolic engineering and metabolic evolution for the development of efficient biomass-producing strain.Systems metabolic engineering guided by flux balance analysis(FBA)was first employed to rationally design mutant strains of Scheffersomyces stipitis with high biomass yield.By experimentally implementing these mutations,the biomass yield was improved by 30%in GPD1,25%in TKL1,30%in CIT1,and 44%in ZWF1 overexpressed mutants compared to wild-type.These designed mutants were further fine-tuned through metabolic evolution resulting in the maximal biomass yield of 0.49 g-cdw/g-glucose,whichmatcheswell with predicted yield phenotype.The constructed mutants are beneficial for biotechnology applications dealing with high cell titer cultivations.This work demonstrates a solid confirmation of systems metabolic engineering in combination with metabolic evolution approach for efficient strain development,which could assist in rapid optimization of cell factory for an economically viable and sustainable bio-based process.展开更多
文摘An 11 year study was conducted to reevaluate the species of stipitate hydnums from the southern United States especially the Great Smoky Mountains and surrounding geography.The genera evaluated included Bankera herein reduced to synonym with Phellodon,Hydnellum,Sarcodon,and Phellodon.Basidiomata of many stipitate hydnum species from this region were abundant but nitrogen depositions during the last 20 years have impacted their occurrences.Therefore it became critical to do a definitive study of these fungi using current taxonomic tools.Many species within Hydnellum and Phellodon have a indeterminate type growth often dependant on environmental conditions making discrimination between closely related species difficult without supportive molecular sequence data.Once sequence data(ITS)was obtained a phylogenetic analyzes were conducted and the sequences were compared to those in GenBank with particular attention to European data.A total of 41 distinct taxa were determined and many of those were confirmed based on morphological data.A specific new combination from Bankera fuligineoalbum to Phellodon fuligineoalbus is made herein.Futhermore,Phellodon brunneoolivaceus is described as new based on morphological and sequence data.
基金Platform Technology grant from National Center for Genetic Engineering and Biotechnology,Thailand(No.P-13-50084).
文摘The development of economically feasible bio-based process requires efficient cell factories capable of producing the desired product at high titer under high-cell-density fermentation.Herein we present a combinatorial approach based on systems metabolic engineering and metabolic evolution for the development of efficient biomass-producing strain.Systems metabolic engineering guided by flux balance analysis(FBA)was first employed to rationally design mutant strains of Scheffersomyces stipitis with high biomass yield.By experimentally implementing these mutations,the biomass yield was improved by 30%in GPD1,25%in TKL1,30%in CIT1,and 44%in ZWF1 overexpressed mutants compared to wild-type.These designed mutants were further fine-tuned through metabolic evolution resulting in the maximal biomass yield of 0.49 g-cdw/g-glucose,whichmatcheswell with predicted yield phenotype.The constructed mutants are beneficial for biotechnology applications dealing with high cell titer cultivations.This work demonstrates a solid confirmation of systems metabolic engineering in combination with metabolic evolution approach for efficient strain development,which could assist in rapid optimization of cell factory for an economically viable and sustainable bio-based process.
