灵长类动物类固醇激素的研究已经在很多物种间展开（Cariso et al.,1999;Lutz et al.,2000;He et al.,2001;Yan and Jiang,2006;Brandon et al.,2008;Lu et al.,2010;Kim et al.,2012）。特别是对于没有明显发情特征的灵长类动物,类固...灵长类动物类固醇激素的研究已经在很多物种间展开（Cariso et al.,1999;Lutz et al.,2000;He et al.,2001;Yan and Jiang,2006;Brandon et al.,2008;Lu et al.,2010;Kim et al.,2012）。特别是对于没有明显发情特征的灵长类动物,类固醇激素的变化可以为它们的繁殖状态提供更多可靠的评估（Fujita et al.,2001）。展开更多
Steroid hormones, alkylphenol ethoxylates（APEOs） and phthalic acid esters（PAEs） are emerging endocrine disrupting chemicals（EDCs） that can interfere with the endocrine function in organisms at low concentrations...Steroid hormones, alkylphenol ethoxylates（APEOs） and phthalic acid esters（PAEs） are emerging endocrine disrupting chemicals（EDCs） that can interfere with the endocrine function in organisms at low concentrations. The occurrence, distribution behavior, removal rate and the fate of 31 target EDCs in sewage treatment plants, which consist of various treatment facilities and receiving water in Hong Kong, were investigated. Estrone,nonylphenol-di-ethoxylate and diethyl phthalate were found to be dominant in each group of influent samples with concentrations ranging from 11–33, 747–3945 and 445–4635 ng/L,respectively. Conversely, progesterone, nonylphenol-mono-ethoxylate and bis（2-ethylhexyl）phthalate were the most abundant in dewatered sludge, with concentrations ranging from 0.9–237, 75–19,743 and 4310–37,016 ng/g（dry weight）. The removal rates of primary sedimentation and disinfection approaches were lower than 30% for most of the chemicals,while those of activated sludge and reverse osmosis were greater than 80% for more than two-thirds of the compounds, noticeably decreasing the estrogenic risk of sewage discharged into the environment. Steroid hormones were removed via biological degradation, while some APEOs and PAEs adsorbed to the sludge. Victoria Harbor poses a low to medium estrogenic risk mainly contributed by estrone and estradiol and deserves attention.展开更多
Objective To investigate the variation of sex hormone and its receptor level in elderly male patients with coronary heart disease (CHD) and to evaluate the correlations between CHD and sex hormone as well as sex hormo...Objective To investigate the variation of sex hormone and its receptor level in elderly male patients with coronary heart disease (CHD) and to evaluate the correlations between CHD and sex hormone as well as sex hormone receptor. Methods Altogether 139 male CHD patients (CHD group) aged 60-92 years and 400 healthy men (control group) aged 60-90 years were included in this cross sectional study. The plasma concentrations of dehydroepiandrosterone sulfate (DHEAS),total testosterone (TT),free testosterone (FT),estradiol (E2),sex hormone binding globulin (SHBG),luteinizing hormone (LH),and follicle-stimulating hormone (FSH) were measured. The androgen receptor (AR) was tested by flow cytometry. Correlations between CHD and levels of sex hormones and AR were analyzed. Results Compared with the control group,the levels of DHEAS,TT,FT,SHBG,and the fluorescence intensity of AR in the CHD group significantly reduced (P<0.05),while the levels of FSH and E2 significantly increased (P<0.01). Age was negatively correlated with TT (r=-0.28,P=0.00) and FT (r=-0.17,P=0.01),while it was positively correlated with SHBG (r=0.14,P=0.04) and E2 (r=0.33,P=0.00). AR fluorescence intensity was negatively correlated with systolic blood pressure (r=-0.12,P=0.01). Binary logistic regression analysis showed that TT,SHBG,and AR were all negatively correlated with CHD (P<0.05). Conclusions Elderly male patients with CHD are found to have low levels of DHEAS,TT,FT,SHBG,and AR,while high concentrations of E2 and FSH. Low levels of TT and SHBG may be the potential risk factors of CHD in elderly men.展开更多
文摘灵长类动物类固醇激素的研究已经在很多物种间展开（Cariso et al.,1999;Lutz et al.,2000;He et al.,2001;Yan and Jiang,2006;Brandon et al.,2008;Lu et al.,2010;Kim et al.,2012）。特别是对于没有明显发情特征的灵长类动物,类固醇激素的变化可以为它们的繁殖状态提供更多可靠的评估（Fujita et al.,2001）。
基金supported by the National Natural Science Foundation of China (NFSC)-Research Grants Committee Joint Research Scheme (N_City U127/12)the National Natural Science Foundation (41276111)+2 种基金the General Research Fund (CityU 11338216, 11100614)Science Technology and Innovation Committee of Shenzhen Municipality (JCYJ20130401145617289)State Key Laboratory in Marine Pollution (SKLMP) Director Discretionary Fund
文摘Steroid hormones, alkylphenol ethoxylates（APEOs） and phthalic acid esters（PAEs） are emerging endocrine disrupting chemicals（EDCs） that can interfere with the endocrine function in organisms at low concentrations. The occurrence, distribution behavior, removal rate and the fate of 31 target EDCs in sewage treatment plants, which consist of various treatment facilities and receiving water in Hong Kong, were investigated. Estrone,nonylphenol-di-ethoxylate and diethyl phthalate were found to be dominant in each group of influent samples with concentrations ranging from 11–33, 747–3945 and 445–4635 ng/L,respectively. Conversely, progesterone, nonylphenol-mono-ethoxylate and bis（2-ethylhexyl）phthalate were the most abundant in dewatered sludge, with concentrations ranging from 0.9–237, 75–19,743 and 4310–37,016 ng/g（dry weight）. The removal rates of primary sedimentation and disinfection approaches were lower than 30% for most of the chemicals,while those of activated sludge and reverse osmosis were greater than 80% for more than two-thirds of the compounds, noticeably decreasing the estrogenic risk of sewage discharged into the environment. Steroid hormones were removed via biological degradation, while some APEOs and PAEs adsorbed to the sludge. Victoria Harbor poses a low to medium estrogenic risk mainly contributed by estrone and estradiol and deserves attention.
基金Supported by the Military Health Care Grant (01AM301, 06G105)
文摘Objective To investigate the variation of sex hormone and its receptor level in elderly male patients with coronary heart disease (CHD) and to evaluate the correlations between CHD and sex hormone as well as sex hormone receptor. Methods Altogether 139 male CHD patients (CHD group) aged 60-92 years and 400 healthy men (control group) aged 60-90 years were included in this cross sectional study. The plasma concentrations of dehydroepiandrosterone sulfate (DHEAS),total testosterone (TT),free testosterone (FT),estradiol (E2),sex hormone binding globulin (SHBG),luteinizing hormone (LH),and follicle-stimulating hormone (FSH) were measured. The androgen receptor (AR) was tested by flow cytometry. Correlations between CHD and levels of sex hormones and AR were analyzed. Results Compared with the control group,the levels of DHEAS,TT,FT,SHBG,and the fluorescence intensity of AR in the CHD group significantly reduced (P<0.05),while the levels of FSH and E2 significantly increased (P<0.01). Age was negatively correlated with TT (r=-0.28,P=0.00) and FT (r=-0.17,P=0.01),while it was positively correlated with SHBG (r=0.14,P=0.04) and E2 (r=0.33,P=0.00). AR fluorescence intensity was negatively correlated with systolic blood pressure (r=-0.12,P=0.01). Binary logistic regression analysis showed that TT,SHBG,and AR were all negatively correlated with CHD (P<0.05). Conclusions Elderly male patients with CHD are found to have low levels of DHEAS,TT,FT,SHBG,and AR,while high concentrations of E2 and FSH. Low levels of TT and SHBG may be the potential risk factors of CHD in elderly men.
文摘目的：建立一种能同时测定血清中多种类固醇激素[包括脱氢表雄酮（ DHEA）、17α－羟孕酮（17α－OHP4）、雄烯二酮（AD）、雌酮（E1）、皮质酮（CORT）、双氢睾酮（DHT）、孕烯醇酮（P5）和17－羟孕烯醇酮（17－OHP5）]的快速、灵敏的稳定同位素稀释高效液相色谱串联质谱（ID－HPLC－MS／MS）方法。方法血清样品经甲基叔丁基醚（MTBE）提取，上清液吹干后加入羟胺进行柱前衍生化反应后检测。采用 Phenomenex C18反相色谱柱和 Phenomenex C18预保护柱。流动相 A 为含0．1％甲酸的水，流动相 B 为含0．1％甲酸的甲醇。质谱采用电喷雾电离（ESI）模式进行离子化，正离子多反应监测模式（MRM）扫描，内标法定量分析。结果血清中8种类固醇激素最低定量限[LLOQ，以信噪比（S／N）≥10为标准]为0．03～0．625 ng／mL；在0．05～50．00 ng／mL范围内线性良好，相关系数（r）均≥0．9980；批内精密度为1．93％～13．81％，批间精密度为4．90％～16．18％，加标回收率为80．0％～130．6％。结论建立了同时测定8种类固醇激素的 ID－HPLC－MS／MS。该法灵敏度高、特异性强、准确且高效，可在10 min 内完成分离和检测，适用于人体血清中类固醇激素的定量分析。
