目的:研究CD4^+CD25^+调节性T细胞(regulatory T cells,Tregs)对乳腺癌细胞上皮间质转化(epithelial-mesenchymal transition,EMT)、细胞迁移侵袭能力,及ALDH1^+干样细胞比例的影响。方法:采用免疫磁珠法分离乳腺癌患者外周血中CD4^+CD2...目的:研究CD4^+CD25^+调节性T细胞(regulatory T cells,Tregs)对乳腺癌细胞上皮间质转化(epithelial-mesenchymal transition,EMT)、细胞迁移侵袭能力,及ALDH1^+干样细胞比例的影响。方法:采用免疫磁珠法分离乳腺癌患者外周血中CD4^+CD25^+Tregs,CD4^+CD25^+Tregs与乳腺癌BT474、MCF-7细胞系共培养(共培养组),BT474、MCF-7单独培养(对照组)。检测共培养组和对照组乳腺癌细胞EMT相关标志物表达的变化,及细胞迁移和侵袭能力的变化。此外,检测BT474细胞中ALDH1^+干样细胞、微球形成能力和自我更新能力的变化。结果:CD4^+CD25^+Tregs诱导BT474和MCF-7细胞间质性标志物表达增高,诱导MCF-7细胞上皮性标志物E-cadherin表达降低。CD4^+CD25^+Tregs诱导BT474和MCF-7细胞迁移和侵袭能力上调。共培养组BT474细胞中ALDH1^+干样细胞比例、微球体形成能力、自我更新能力较对照组增强。结论:CD4^+CD25^+Tregs可诱导乳腺癌细胞发生EMT,增强细胞体外迁移和侵袭能力,同时促进ALDH1^+干样细胞增加。展开更多
BACKGROUND： S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells （MSCs） that can secrete S100 may provide a beneficial microenvironment for neural...BACKGROUND： S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells （MSCs） that can secrete S100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE： To explore the changes in S100 expression during rat MSCs differentiation into Schwann ceils in vitro. DESIGN, TIME AND SETTING： This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALS： The rabbit anti-S100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat S100 monoclonal antibody was purchased from Sigma, USA. METHODS： MSCs were cultured from adult Sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was measured by flow cytometry. MSCs were induced to differentiate into SC cells. SC cells were stained for S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. S100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURES： S100 protein and mRNA expression. RESULTS： MSCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MSCs were at the G0/G1 phase of the cell cycle. After induction, MSCs displayed morphology changes similar to Schwann cells. Moreover, induction increased S100 mRNA levels. Immunofluorescence showed that MSCs expressed S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased S100 protein levels compared with untreated MSCs. CONCLUSION： MSCs are capable of differentiating into Schwann cells-like cells under conditional induction in vitro, with increasing S100 mRNA and protein expression.展开更多
文摘目的:研究CD4^+CD25^+调节性T细胞(regulatory T cells,Tregs)对乳腺癌细胞上皮间质转化(epithelial-mesenchymal transition,EMT)、细胞迁移侵袭能力,及ALDH1^+干样细胞比例的影响。方法:采用免疫磁珠法分离乳腺癌患者外周血中CD4^+CD25^+Tregs,CD4^+CD25^+Tregs与乳腺癌BT474、MCF-7细胞系共培养(共培养组),BT474、MCF-7单独培养(对照组)。检测共培养组和对照组乳腺癌细胞EMT相关标志物表达的变化,及细胞迁移和侵袭能力的变化。此外,检测BT474细胞中ALDH1^+干样细胞、微球形成能力和自我更新能力的变化。结果:CD4^+CD25^+Tregs诱导BT474和MCF-7细胞间质性标志物表达增高,诱导MCF-7细胞上皮性标志物E-cadherin表达降低。CD4^+CD25^+Tregs诱导BT474和MCF-7细胞迁移和侵袭能力上调。共培养组BT474细胞中ALDH1^+干样细胞比例、微球体形成能力、自我更新能力较对照组增强。结论:CD4^+CD25^+Tregs可诱导乳腺癌细胞发生EMT,增强细胞体外迁移和侵袭能力,同时促进ALDH1^+干样细胞增加。
基金the National High-Tech Research & Development Program of China, No. 2006AA02A128the National Natural Science Foundation of China, No. 30670667
文摘BACKGROUND： S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells （MSCs） that can secrete S100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE： To explore the changes in S100 expression during rat MSCs differentiation into Schwann ceils in vitro. DESIGN, TIME AND SETTING： This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALS： The rabbit anti-S100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat S100 monoclonal antibody was purchased from Sigma, USA. METHODS： MSCs were cultured from adult Sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was measured by flow cytometry. MSCs were induced to differentiate into SC cells. SC cells were stained for S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. S100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURES： S100 protein and mRNA expression. RESULTS： MSCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MSCs were at the G0/G1 phase of the cell cycle. After induction, MSCs displayed morphology changes similar to Schwann cells. Moreover, induction increased S100 mRNA levels. Immunofluorescence showed that MSCs expressed S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased S100 protein levels compared with untreated MSCs. CONCLUSION： MSCs are capable of differentiating into Schwann cells-like cells under conditional induction in vitro, with increasing S100 mRNA and protein expression.
