Background：Mesenchymal stem cells （MSCs） transplantation has been proven to have therapeutic potential for acute liver failure （ALF）.However,the mechanism remains controversial.Recently,modulation of inflammation...Background：Mesenchymal stem cells （MSCs） transplantation has been proven to have therapeutic potential for acute liver failure （ALF）.However,the mechanism remains controversial.Recently,modulation of inflammation by MSCs has been regarded as a crucial mechanism.The aim of the present study was to explore the soluble cytokines secreted by MSCs and their therapeutic effects in ALF.Methods：MSCs isolated from Sprague-Dawley rats were identified by fluorescence-activated cell sorting analysis.Conditioned medium derived from MSCs （MSCs-CM） was collected and analyzed by a cytokine microarray.MSCs and MSCs-CM were transplanted into rats with D-galactosamine-induced ALF.Liver function,survival rate,histology,and inflammatory factors were determined.Exogenous recombinant rat interleukin （IL）-10,anti-rat IL-10 antibody,and AG490 （signal transducer and activator of transcription 3 [STAT3] signaling pathway inhibitor） were administered to explore the therapeutic mechanism of MSCs-CM.Statistical analysis was performed with SPSS version 19.0,and all data were analyzed by the independent-sample t-test.Results：There are statistical differences of the survival curve between ALF＋MSCs group and ALF＋Dulbecco＆#39;s modified Eagle＆#39;s medium （DMEM） group,as well as ALF＋MSCs-CM group and ALF＋DMEM group （all P 〈 0.05）.Serum alanine aminotransferase （ALT） level in the ALF＋MSCs and ALF＋MSCs-CM groups was lower than that in the ALF＋DMEM group （865.53＆#177;52.80 vs.1709.75＆#177;372.12 U/L and 964.72＆#177;414.59 vs.1709.75＆#177;372.12 U/L,respectively,all P 〈 0.05）;meanwhile,serum aspartate aminotransferase （AST） level in the ALF＋MSCs and ALF＋MSCs-CM groups was lower than that in the ALF＋DMEM group （2440.83＆#177;511.94 vs.4234.35＆#177;807.30 U/L and 2739.83＆#177;587.33 vs.4234.35＆#177;807.30 U/L,respectively,all P 〈 0.05）.Furthermore,MSCs or MSCs-CM treatment significantly reduced serum interferon-γ （IFN-γ）,IL-1β,IL-6 levels and increased 展开更多
Background： Researchers initially proposed the substitution ofapoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells （MSCs） intraarticularly. This effect was termed as bio-resurfaci...Background： Researchers initially proposed the substitution ofapoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells （MSCs） intraarticularly. This effect was termed as bio-resurfacing. Little evidence supporting the treatment ofosteoarthritis （OA） by the delivery ofa MSC suspension exists. The aim of this study was to investigate the effects of injecting allogenic MSCs intraarticularly in a rat OA model and to evaluate the influence of immobility on the effects of this treatment. Methods： We established a rat knee OA model after 4 and 6 weeks and cultured primary bone marrow MSCs. A MSC suspension was injected into the articular space once per week for 3 weeks. A subgroup of knee joints was immobilized for 3 days after each injection, while the remaining joints were nonimmobilized. We used toluidine blue staining, Mankin scores, and TdT-mediated dUTP-biotin nick end labeling staining to evaluate the therapeutic effect of the injections. Comparisons between the therapy side and the control side of the knee joint were made using paired t-test, and comparisons between the immobilized and nonimmobilized subgroups were made using the unpaired t-test. A P value 〈 0.05 was considered significant. Results： The three investigative approaches revealed less degeneration on the therapy sides of the knee joints than the control sides in both the 4- and 6-week groups （P 〈 0.05）, regardless of immobilization. No significant differences were observed between the immobilized and nonimmobilized subgroups （P 〉 0.05）. Conclusions： Therapy involving the intraarticular injection of allogenic MSCs promoted cartilage repair in a rat arthritis model, and 3-day immobility alter injection had little effect on this therapy.展开更多
This paper deals with the problems of consistency and strong consistency of the maximum likelihood estimators of the mean and variance of the drift fractional Brownian motions observed at discrete time instants. Both ...This paper deals with the problems of consistency and strong consistency of the maximum likelihood estimators of the mean and variance of the drift fractional Brownian motions observed at discrete time instants. Both the central limit theorem and the Berry-Ess′een bounds for these estimators are obtained by using the Stein’s method via Malliavin calculus.展开更多
In this paper, two different methods are used to study the cyclic structure solution and the optimal approximation of the quaternion Stein equation AXB - X = F . Firstly, the matrix equation equivalent to the ta...In this paper, two different methods are used to study the cyclic structure solution and the optimal approximation of the quaternion Stein equation AXB - X = F . Firstly, the matrix equation equivalent to the target structure matrix is constructed by using the complex decomposition of the quaternion matrix, to obtain the necessary and sufficient conditions for the existence of the cyclic solution of the equation and the expression of the general solution. Secondly, the Stein equation is converted into the Sylvester equation by adding the necessary parameters, and the condition for the existence of a cyclic solution and the expression of the equation’s solution are then obtained by using the real decomposition of the quaternion matrix and the Kronecker product of the matrix. At the same time, under the condition that the solution set is non-empty, the optimal approximation solution to the given quaternion circulant matrix is obtained by using the property of Frobenius norm property. Numerical examples are given to verify the correctness of the theoretical results and the feasibility of the proposed method. .展开更多
An effective de-noising method for fiber optic gyroscopes （FOGs） is proposed. This method is based on second-generation Daubechies D4 （DB4） wavelet transform （WT） and level-dependent threshold estimator called S...An effective de-noising method for fiber optic gyroscopes （FOGs） is proposed. This method is based on second-generation Daubechies D4 （DB4） wavelet transform （WT） and level-dependent threshold estimator called Stein＇s unbiased risk estimator （SURE）. The whole approach consists of three critical parts： wavelet decomposition module, parameters estimation module and SURE de-noising module. First, DB4 wavelet is selected as lifting base of the second-generation wavelet in the decomposition module. Second, in the parameters estimation module, maximum likelihood estimation （MLE） is used for stochastic noise parameters estimation. Third, combined with soft threshold de-noising technique, the SURE de-noising module is designed. For comparison, both the traditional universal threshold wavelet and the second-generation Harr wavelet method are also investigated. The experiment results show that the computation cost is 40% less than that of the traditional wavelet method. The standard deviation of de-noised FOG signal is 0.012 and the three noise terms such as angle random walk, bias instability and quantization noise are reduced to 0.007 2°/√h, 0.004 1° / h, and 0.008 1°, respectively.展开更多
A new Koppelman-Leray-Norguet formula of (p,q) differential forms for a strictly pseudoconvex polyhedron with not necessarily smooth boundary on a Stein manifold is obtained, and an integral representation for the sol...A new Koppelman-Leray-Norguet formula of (p,q) differential forms for a strictly pseudoconvex polyhedron with not necessarily smooth boundary on a Stein manifold is obtained, and an integral representation for the solution of -equation on this domain which does not involve integrals on boundary is given, so one can avoid complex estimates of boundary integrals.展开更多
Let Tn be the number of triangles in the random intersection graph G(n,m,p).When the mean of Tn is bounded,we obtain an upper bound on the total variation distance between Tn and a Poisson distribution.When the mean o...Let Tn be the number of triangles in the random intersection graph G(n,m,p).When the mean of Tn is bounded,we obtain an upper bound on the total variation distance between Tn and a Poisson distribution.When the mean of Tn tends to infinity,the Stein–Tikhomirov method is used to bound the error for the normal approximation of Tn with respect to the Kolmogorov metric.展开更多
As a representative modernist writer of the early 20th century,Gertrude Stein creatively established a unique experimental writing style to support her modernist expression.This paper attempts to analyze the Oriental ...As a representative modernist writer of the early 20th century,Gertrude Stein creatively established a unique experimental writing style to support her modernist expression.This paper attempts to analyze the Oriental elements,especially elements and images related to“China”in her works.Stein’s uses these elements to construct a systematic and complex matrix of aesthetics and politics.“China”in Stein’s matrix is an essential source of her so-called“the oriental peaceful penetration”and a significant lens for her ideological expression and political thoughts.Although Stein emphasizes her Oriental components in the source of identity,she always places herself in a Western position,ignoring cultural and political expressions in the real Chinese context and turning“China”into a useful tool in the collective fantasies of the West.展开更多
2008年5月12日,灾难性的汶川7.9级大地震袭击了青藏高原东缘,造成四川盆地西部多个大城市房屋倒塌和大量人员伤亡。如此大的地震之后,地壳应力的重新调整通常会导致破坏性地震发生(Steinet al,1997;Stein,1999;McCloskey et al,2005;Par...2008年5月12日,灾难性的汶川7.9级大地震袭击了青藏高原东缘,造成四川盆地西部多个大城市房屋倒塌和大量人员伤亡。如此大的地震之后,地壳应力的重新调整通常会导致破坏性地震发生(Steinet al,1997;Stein,1999;McCloskey et al,2005;Parsonset al,2000;Parsons,2002)。5月12日地震的主震造成龙门山和四川盆地连接处最大位错量达9m,说明复杂的走滑和逆冲运动(Ji and Hayes,2008)为该地区的特征(Burchfiel et al,1995;Densmore et al,2007)。四川盆地及其周边也有其他活跃的走滑和逆冲断层相互交错。本文利用地震断层模型计算了由2008年5月12日汶川地震引起的同震应力变化,结果显示很多地区应力显著增加。快速给出这种应力变化图像有助于确定未来可能发生强余震的断层段。展开更多
Let （X, d,μ） be a metric measure space endowed with a metric d and a nonnegative Borel doubling measure μ. Let L be a second order non-negative self-adjoint operator on L^2（X）. Assume that the semigroup e^-tL ge...Let （X, d,μ） be a metric measure space endowed with a metric d and a nonnegative Borel doubling measure μ. Let L be a second order non-negative self-adjoint operator on L^2（X）. Assume that the semigroup e^-tL generated by L satisfies the Davies-Gaffney estimates. Also, assume that L satisfies Plancherel type estimate. Under these conditions, we show that Stein＇s square function Gδ（L） arising from Bochner-Riesz means associated to L is bounded from the Hardy spaces HL^p（X） to L^p（X） for all 0 〈 p ≤ 1.展开更多
Using the method of localization, the authors obtain the permutation formula of singular integrals with Bochner-Martinelli kernel for a relative compact domain with C^（1） smooth boundary on a Stein manifold. As an a...Using the method of localization, the authors obtain the permutation formula of singular integrals with Bochner-Martinelli kernel for a relative compact domain with C^（1） smooth boundary on a Stein manifold. As an application the authors discuss the regularization problem for linear singular integral equations with Bochner-Martinelli kernel and variable coefficients; using permutation formula, the singular integral equation can be reduced to a fredholm equation.展开更多
Two fundamental problems on the invariant holomorphic extensions have been posed, which are naturally arose from our solution of the extended future tube conjecture and closely and deeply related to the general theory...Two fundamental problems on the invariant holomorphic extensions have been posed, which are naturally arose from our solution of the extended future tube conjecture and closely and deeply related to the general theory of Stein manifolds due to Cartan-Serre. In this paper, the relationship is presented between the two problems, the motivation of considering the problems, and the methods to approach the problems. We have also posed some questions and conjectures related to this two problems.展开更多
基金This study was supported by grants from the National Natural Science Foundation of China，the Natural Science Foundation of Jiangsu Province,China
文摘Background：Mesenchymal stem cells （MSCs） transplantation has been proven to have therapeutic potential for acute liver failure （ALF）.However,the mechanism remains controversial.Recently,modulation of inflammation by MSCs has been regarded as a crucial mechanism.The aim of the present study was to explore the soluble cytokines secreted by MSCs and their therapeutic effects in ALF.Methods：MSCs isolated from Sprague-Dawley rats were identified by fluorescence-activated cell sorting analysis.Conditioned medium derived from MSCs （MSCs-CM） was collected and analyzed by a cytokine microarray.MSCs and MSCs-CM were transplanted into rats with D-galactosamine-induced ALF.Liver function,survival rate,histology,and inflammatory factors were determined.Exogenous recombinant rat interleukin （IL）-10,anti-rat IL-10 antibody,and AG490 （signal transducer and activator of transcription 3 [STAT3] signaling pathway inhibitor） were administered to explore the therapeutic mechanism of MSCs-CM.Statistical analysis was performed with SPSS version 19.0,and all data were analyzed by the independent-sample t-test.Results：There are statistical differences of the survival curve between ALF＋MSCs group and ALF＋Dulbecco＆#39;s modified Eagle＆#39;s medium （DMEM） group,as well as ALF＋MSCs-CM group and ALF＋DMEM group （all P 〈 0.05）.Serum alanine aminotransferase （ALT） level in the ALF＋MSCs and ALF＋MSCs-CM groups was lower than that in the ALF＋DMEM group （865.53＆#177;52.80 vs.1709.75＆#177;372.12 U/L and 964.72＆#177;414.59 vs.1709.75＆#177;372.12 U/L,respectively,all P 〈 0.05）;meanwhile,serum aspartate aminotransferase （AST） level in the ALF＋MSCs and ALF＋MSCs-CM groups was lower than that in the ALF＋DMEM group （2440.83＆#177;511.94 vs.4234.35＆#177;807.30 U/L and 2739.83＆#177;587.33 vs.4234.35＆#177;807.30 U/L,respectively,all P 〈 0.05）.Furthermore,MSCs or MSCs-CM treatment significantly reduced serum interferon-γ （IFN-γ）,IL-1β,IL-6 levels and increased
文摘Background： Researchers initially proposed the substitution ofapoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells （MSCs） intraarticularly. This effect was termed as bio-resurfacing. Little evidence supporting the treatment ofosteoarthritis （OA） by the delivery ofa MSC suspension exists. The aim of this study was to investigate the effects of injecting allogenic MSCs intraarticularly in a rat OA model and to evaluate the influence of immobility on the effects of this treatment. Methods： We established a rat knee OA model after 4 and 6 weeks and cultured primary bone marrow MSCs. A MSC suspension was injected into the articular space once per week for 3 weeks. A subgroup of knee joints was immobilized for 3 days after each injection, while the remaining joints were nonimmobilized. We used toluidine blue staining, Mankin scores, and TdT-mediated dUTP-biotin nick end labeling staining to evaluate the therapeutic effect of the injections. Comparisons between the therapy side and the control side of the knee joint were made using paired t-test, and comparisons between the immobilized and nonimmobilized subgroups were made using the unpaired t-test. A P value 〈 0.05 was considered significant. Results： The three investigative approaches revealed less degeneration on the therapy sides of the knee joints than the control sides in both the 4- and 6-week groups （P 〈 0.05）, regardless of immobilization. No significant differences were observed between the immobilized and nonimmobilized subgroups （P 〉 0.05）. Conclusions： Therapy involving the intraarticular injection of allogenic MSCs promoted cartilage repair in a rat arthritis model, and 3-day immobility alter injection had little effect on this therapy.
基金supported by the National Science Foundations (DMS0504783 DMS0604207)National Science Fund for Distinguished Young Scholars of China (70825005)
文摘This paper deals with the problems of consistency and strong consistency of the maximum likelihood estimators of the mean and variance of the drift fractional Brownian motions observed at discrete time instants. Both the central limit theorem and the Berry-Ess′een bounds for these estimators are obtained by using the Stein’s method via Malliavin calculus.
文摘In this paper, two different methods are used to study the cyclic structure solution and the optimal approximation of the quaternion Stein equation AXB - X = F . Firstly, the matrix equation equivalent to the target structure matrix is constructed by using the complex decomposition of the quaternion matrix, to obtain the necessary and sufficient conditions for the existence of the cyclic solution of the equation and the expression of the general solution. Secondly, the Stein equation is converted into the Sylvester equation by adding the necessary parameters, and the condition for the existence of a cyclic solution and the expression of the equation’s solution are then obtained by using the real decomposition of the quaternion matrix and the Kronecker product of the matrix. At the same time, under the condition that the solution set is non-empty, the optimal approximation solution to the given quaternion circulant matrix is obtained by using the property of Frobenius norm property. Numerical examples are given to verify the correctness of the theoretical results and the feasibility of the proposed method. .
基金Supported by the Aerospace Science and Technology Innovation Foundation of China (2006)
文摘An effective de-noising method for fiber optic gyroscopes （FOGs） is proposed. This method is based on second-generation Daubechies D4 （DB4） wavelet transform （WT） and level-dependent threshold estimator called Stein＇s unbiased risk estimator （SURE）. The whole approach consists of three critical parts： wavelet decomposition module, parameters estimation module and SURE de-noising module. First, DB4 wavelet is selected as lifting base of the second-generation wavelet in the decomposition module. Second, in the parameters estimation module, maximum likelihood estimation （MLE） is used for stochastic noise parameters estimation. Third, combined with soft threshold de-noising technique, the SURE de-noising module is designed. For comparison, both the traditional universal threshold wavelet and the second-generation Harr wavelet method are also investigated. The experiment results show that the computation cost is 40% less than that of the traditional wavelet method. The standard deviation of de-noised FOG signal is 0.012 and the three noise terms such as angle random walk, bias instability and quantization noise are reduced to 0.007 2°/√h, 0.004 1° / h, and 0.008 1°, respectively.
基金Supported by the National Natural Science Foundation and Mathematical "Tian Yuan" Foundation of China and the Natural Science Foundation of Fujian (Grant No. 10271097, TY10126033, F0110012)
文摘A new Koppelman-Leray-Norguet formula of (p,q) differential forms for a strictly pseudoconvex polyhedron with not necessarily smooth boundary on a Stein manifold is obtained, and an integral representation for the solution of -equation on this domain which does not involve integrals on boundary is given, so one can avoid complex estimates of boundary integrals.
文摘Let Tn be the number of triangles in the random intersection graph G(n,m,p).When the mean of Tn is bounded,we obtain an upper bound on the total variation distance between Tn and a Poisson distribution.When the mean of Tn tends to infinity,the Stein–Tikhomirov method is used to bound the error for the normal approximation of Tn with respect to the Kolmogorov metric.
文摘As a representative modernist writer of the early 20th century,Gertrude Stein creatively established a unique experimental writing style to support her modernist expression.This paper attempts to analyze the Oriental elements,especially elements and images related to“China”in her works.Stein’s uses these elements to construct a systematic and complex matrix of aesthetics and politics.“China”in Stein’s matrix is an essential source of her so-called“the oriental peaceful penetration”and a significant lens for her ideological expression and political thoughts.Although Stein emphasizes her Oriental components in the source of identity,she always places herself in a Western position,ignoring cultural and political expressions in the real Chinese context and turning“China”into a useful tool in the collective fantasies of the West.
文摘2008年5月12日,灾难性的汶川7.9级大地震袭击了青藏高原东缘,造成四川盆地西部多个大城市房屋倒塌和大量人员伤亡。如此大的地震之后,地壳应力的重新调整通常会导致破坏性地震发生(Steinet al,1997;Stein,1999;McCloskey et al,2005;Parsonset al,2000;Parsons,2002)。5月12日地震的主震造成龙门山和四川盆地连接处最大位错量达9m,说明复杂的走滑和逆冲运动(Ji and Hayes,2008)为该地区的特征(Burchfiel et al,1995;Densmore et al,2007)。四川盆地及其周边也有其他活跃的走滑和逆冲断层相互交错。本文利用地震断层模型计算了由2008年5月12日汶川地震引起的同震应力变化,结果显示很多地区应力显著增加。快速给出这种应力变化图像有助于确定未来可能发生强余震的断层段。
文摘Let （X, d,μ） be a metric measure space endowed with a metric d and a nonnegative Borel doubling measure μ. Let L be a second order non-negative self-adjoint operator on L^2（X）. Assume that the semigroup e^-tL generated by L satisfies the Davies-Gaffney estimates. Also, assume that L satisfies Plancherel type estimate. Under these conditions, we show that Stein＇s square function Gδ（L） arising from Bochner-Riesz means associated to L is bounded from the Hardy spaces HL^p（X） to L^p（X） for all 0 〈 p ≤ 1.
基金The project was supported by the Natural Science Foundation of Fujian Province of China (Z0511002)the National Science Foundation of China (10271097,10571144)+1 种基金Foundation of Tianyuan (10526033)Chen L P, the Corresponding author
文摘Using the method of localization, the authors obtain the permutation formula of singular integrals with Bochner-Martinelli kernel for a relative compact domain with C^（1） smooth boundary on a Stein manifold. As an application the authors discuss the regularization problem for linear singular integral equations with Bochner-Martinelli kernel and variable coefficients; using permutation formula, the singular integral equation can be reduced to a fredholm equation.
文摘Two fundamental problems on the invariant holomorphic extensions have been posed, which are naturally arose from our solution of the extended future tube conjecture and closely and deeply related to the general theory of Stein manifolds due to Cartan-Serre. In this paper, the relationship is presented between the two problems, the motivation of considering the problems, and the methods to approach the problems. We have also posed some questions and conjectures related to this two problems.
