为了探究4种常用杀虫剂对芒果蓟马的防效,本研究在百色田阳芒果基地开展不同杀虫剂防治芒果嫩梢蓟马的田间试验。结果表明20%多杀·甲维盐SC 30 g a.i.·hm^(-2)在药后7 d内的防效均在78%以上,防效最佳,其次是6%乙基多杀菌素SC ...为了探究4种常用杀虫剂对芒果蓟马的防效,本研究在百色田阳芒果基地开展不同杀虫剂防治芒果嫩梢蓟马的田间试验。结果表明20%多杀·甲维盐SC 30 g a.i.·hm^(-2)在药后7 d内的防效均在78%以上,防效最佳,其次是6%乙基多杀菌素SC 9 g a.i.·hm^(-2)在药后7 d内的防效在71%以上,20%吡虫啉SLX和25%吡虫·异丙威WP两种浓度处理的防效均在60%以下。因此在芒果蓟马防治上建议将20%多杀·甲维盐和6%乙基多杀菌素与其他杀虫剂轮换使用,同时加强区域统一防治,以提高防效。展开更多
Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora spinosa.However,their large-...Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora spinosa.However,their large-scale overproduction is hindered by poorly understood bottlenecks in optimizing the original strain,and poor adaptability of the heterologous strain to the production of spinosyn.In this study,we genetically engineered heterologous spinosyn-producer Streptomyces albus J1074 and optimized the fermentation to improve the production of spinosad(spinosyn A and spinosyn D)based on our previous work.We systematically investigated the result of overexpressing polyketide synthase genes(spnA,B,C,D,E)using a constitutive promoter on the spinosad titer in S.albus J1074.The supply of polyketide synthase precursors was then increased to further improve spinosad production.Finally,increasing or replacing the carbon source of the culture medium resulted in a final spinosad titer of~70 mg/L,which is the highest titer of spinosad achieved in heterologous Streptomyces species.This research provides useful strategies for efficient heterologous production of natural products.展开更多
文摘为了探究4种常用杀虫剂对芒果蓟马的防效,本研究在百色田阳芒果基地开展不同杀虫剂防治芒果嫩梢蓟马的田间试验。结果表明20%多杀·甲维盐SC 30 g a.i.·hm^(-2)在药后7 d内的防效均在78%以上,防效最佳,其次是6%乙基多杀菌素SC 9 g a.i.·hm^(-2)在药后7 d内的防效在71%以上,20%吡虫啉SLX和25%吡虫·异丙威WP两种浓度处理的防效均在60%以下。因此在芒果蓟马防治上建议将20%多杀·甲维盐和6%乙基多杀菌素与其他杀虫剂轮换使用,同时加强区域统一防治,以提高防效。
基金This work was supported by the National Key R&D Program of China[grant number 2018YFA0900400]the National Natural Science Foundation of China[grant number 31670090],and J1 Biotech Co.,Ltd.
文摘Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora spinosa.However,their large-scale overproduction is hindered by poorly understood bottlenecks in optimizing the original strain,and poor adaptability of the heterologous strain to the production of spinosyn.In this study,we genetically engineered heterologous spinosyn-producer Streptomyces albus J1074 and optimized the fermentation to improve the production of spinosad(spinosyn A and spinosyn D)based on our previous work.We systematically investigated the result of overexpressing polyketide synthase genes(spnA,B,C,D,E)using a constitutive promoter on the spinosad titer in S.albus J1074.The supply of polyketide synthase precursors was then increased to further improve spinosad production.Finally,increasing or replacing the carbon source of the culture medium resulted in a final spinosad titer of~70 mg/L,which is the highest titer of spinosad achieved in heterologous Streptomyces species.This research provides useful strategies for efficient heterologous production of natural products.
