大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白中最为精制的形式,广泛应用于食品工业,在不同的产品中表现出不同的功能性。大豆分离蛋白可以用物理、化学、酶法和生物工程方法进行改性。结果表明,大豆分离蛋白经过改性后能拓宽大豆...大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白中最为精制的形式,广泛应用于食品工业,在不同的产品中表现出不同的功能性。大豆分离蛋白可以用物理、化学、酶法和生物工程方法进行改性。结果表明,大豆分离蛋白经过改性后能拓宽大豆分离蛋白在食品工业中的应用及达到人们所希望的功能特性。展开更多
The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated...The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77?mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016?mol·L -1 and V max =0.765?mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30?min.The good fitness between predicted and measured results confirmed the usefulness of the model.展开更多
大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白最为精制形式,广泛应用于食品工业,在不同产品中表现出不同功能。该文综述近年来有关大豆分离蛋白物理、化学、生物方法改性研究,并对生物工程改性作一简单介绍;大豆分离蛋白经改性后...大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白最为精制形式,广泛应用于食品工业,在不同产品中表现出不同功能。该文综述近年来有关大豆分离蛋白物理、化学、生物方法改性研究,并对生物工程改性作一简单介绍;大豆分离蛋白经改性后能拓展大豆分离蛋白在食品工业中应用及达到人们所希望功能特性。展开更多
以大豆分离蛋白为基础原料,运用多种蛋白分析检测手段,深入探讨70、80、85、90、100℃热处理条件对大豆分离蛋白体外模拟消化特性影响。从蛋白质水解度(the degree of hydrolysis,DH)曲线来看,随着热处理温度的升高,DH曲线呈现先上升后...以大豆分离蛋白为基础原料,运用多种蛋白分析检测手段,深入探讨70、80、85、90、100℃热处理条件对大豆分离蛋白体外模拟消化特性影响。从蛋白质水解度(the degree of hydrolysis,DH)曲线来看,随着热处理温度的升高,DH曲线呈现先上升后下降的变化趋势,而长时间也会使DH呈现下降的趋势。傅里叶转换红外光谱及拉曼光谱图分析显示,经不同温度热预处理样品的消化产物二级结构中α-螺旋结构含量较未经预处理样品的高,而β-折叠结构含量降低,β-转角与无规卷曲结构含量变化不明显;随着时间的延长,α-螺旋结构含量呈先升高后降低的变化趋势,而β-折叠结构含量先降低后升高。拉曼光谱中酪氨酸峰变化较小且不规律,热处理使色氨酸残基更趋近于"包埋态"。展开更多
文摘大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白中最为精制的形式,广泛应用于食品工业,在不同的产品中表现出不同的功能性。大豆分离蛋白可以用物理、化学、酶法和生物工程方法进行改性。结果表明,大豆分离蛋白经过改性后能拓宽大豆分离蛋白在食品工业中的应用及达到人们所希望的功能特性。
文摘The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77?mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016?mol·L -1 and V max =0.765?mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30?min.The good fitness between predicted and measured results confirmed the usefulness of the model.
文摘大豆分离蛋白(soy protein isolate,SPI)是大豆蛋白最为精制形式,广泛应用于食品工业,在不同产品中表现出不同功能。该文综述近年来有关大豆分离蛋白物理、化学、生物方法改性研究,并对生物工程改性作一简单介绍;大豆分离蛋白经改性后能拓展大豆分离蛋白在食品工业中应用及达到人们所希望功能特性。
文摘以大豆分离蛋白为基础原料,运用多种蛋白分析检测手段,深入探讨70、80、85、90、100℃热处理条件对大豆分离蛋白体外模拟消化特性影响。从蛋白质水解度(the degree of hydrolysis,DH)曲线来看,随着热处理温度的升高,DH曲线呈现先上升后下降的变化趋势,而长时间也会使DH呈现下降的趋势。傅里叶转换红外光谱及拉曼光谱图分析显示,经不同温度热预处理样品的消化产物二级结构中α-螺旋结构含量较未经预处理样品的高,而β-折叠结构含量降低,β-转角与无规卷曲结构含量变化不明显;随着时间的延长,α-螺旋结构含量呈先升高后降低的变化趋势,而β-折叠结构含量先降低后升高。拉曼光谱中酪氨酸峰变化较小且不规律,热处理使色氨酸残基更趋近于"包埋态"。
