根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基...根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较,证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录,序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。展开更多
Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasm...Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasmid pHP23 carrying the NPTⅡ gene which encodes resistance to antibiotic G 418. Protoplast derived colonies were selected in presence of the inhibitor. Plants were regenerated and transplanted into soil in the green house. The presence of foreign gene in the regenerated plants was verified by PCR and Southern analysis.展开更多
文摘根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较,证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录,序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。
文摘Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasmid pHP23 carrying the NPTⅡ gene which encodes resistance to antibiotic G 418. Protoplast derived colonies were selected in presence of the inhibitor. Plants were regenerated and transplanted into soil in the green house. The presence of foreign gene in the regenerated plants was verified by PCR and Southern analysis.
