With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual microbial.genotypes and functional expression,whic...With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual microbial.genotypes and functional expression,which deepens our understanding of microorganisms.In this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as well.Moreover,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development status.Despite its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.展开更多
Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of anim...Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of animal husbandry in recent years.This technology provides an essential basis for the early selection of livestock and poultry and the selection of excellent characters,showing that it has great application potential and broad prospects in animal husbandry research and production.Although scRNA-seq technology has established a wide range of commercial production services at home and abroad,the standardization status of this technology in animal husbandry needs further exploration,as it can provide clues and foundations for the application of scRNA-seq technology in animal husbandry.展开更多
The microenvironment at the maternal-fetal interface is optimized to facilitate the development and survival of the fetus during pregnancy.It involves a balance between cell populations and interactions of the fetal p...The microenvironment at the maternal-fetal interface is optimized to facilitate the development and survival of the fetus during pregnancy.It involves a balance between cell populations and interactions of the fetal placenta with various cell types(ie,stromal cells,endothelial cells,immune cells,and fibroblasts)that are embedded in the maternal endometrium/decidua.Aberrant shifts in cell populations and deranged cell-cell interactions are closely related to pregnancy disorders.Thus,analysis of the dynamic changes in cell populations and their interactions at the maternal-fetal interface in normal and complicated pregnancies is essential to provide insights into the fundamental processes involved in the establishment and maintenance of normal pregnancy,and how these processes are dysregulated.Thus,informing novel pathways for therapeutic targets of pregnancy complications.Single-cell sequencing(SCS)is a powerful tool for transcriptome analysis at single-cell resolution.Combined with information on the developmental trajectory and function of different cell populations,SCS can provide an unparalleled opportunity for refining the spatiotemporal cell atlas to elaborate dynamic changes in cell populations and their interactions in tissues that consist of highly heterogeneous cell populations such as the maternal-fetal interface.This minireview briefly summarizes traditional methods and their limitations for analyzing maternal-fetal interface cell-cell interactions,and introduces the current applications,advantages,limitations,and prospective applications of SCS in research on maternal-fetal interactions.展开更多
基金supported by the Key Research and Development Project of Zhejiang Province,China(No.2022C03026)the Zhejiang Medical and Health Technology Project(China)(No.2023RC274)Public Welfare Technology Application Research Program of Huzhou,China(No.2021GY15).
文摘With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual microbial.genotypes and functional expression,which deepens our understanding of microorganisms.In this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as well.Moreover,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development status.Despite its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.
文摘Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of animal husbandry in recent years.This technology provides an essential basis for the early selection of livestock and poultry and the selection of excellent characters,showing that it has great application potential and broad prospects in animal husbandry research and production.Although scRNA-seq technology has established a wide range of commercial production services at home and abroad,the standardization status of this technology in animal husbandry needs further exploration,as it can provide clues and foundations for the application of scRNA-seq technology in animal husbandry.
基金supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region(2018GXNSFDA050017,2019GXNSFFA245013)the National Natural Science Foundation of China(81871172)the Guangxi Medical University Training Program for Distinguished Young Scholars.
文摘The microenvironment at the maternal-fetal interface is optimized to facilitate the development and survival of the fetus during pregnancy.It involves a balance between cell populations and interactions of the fetal placenta with various cell types(ie,stromal cells,endothelial cells,immune cells,and fibroblasts)that are embedded in the maternal endometrium/decidua.Aberrant shifts in cell populations and deranged cell-cell interactions are closely related to pregnancy disorders.Thus,analysis of the dynamic changes in cell populations and their interactions at the maternal-fetal interface in normal and complicated pregnancies is essential to provide insights into the fundamental processes involved in the establishment and maintenance of normal pregnancy,and how these processes are dysregulated.Thus,informing novel pathways for therapeutic targets of pregnancy complications.Single-cell sequencing(SCS)is a powerful tool for transcriptome analysis at single-cell resolution.Combined with information on the developmental trajectory and function of different cell populations,SCS can provide an unparalleled opportunity for refining the spatiotemporal cell atlas to elaborate dynamic changes in cell populations and their interactions in tissues that consist of highly heterogeneous cell populations such as the maternal-fetal interface.This minireview briefly summarizes traditional methods and their limitations for analyzing maternal-fetal interface cell-cell interactions,and introduces the current applications,advantages,limitations,and prospective applications of SCS in research on maternal-fetal interactions.
