Sensitive and specific bioassays of tumor markers are critical for early cancer detection and treatment.In recent years,lanthanide(Ln3+)doped upconversion nanoparticles(UCNPs)have attracted wide attentions in tumor ma...Sensitive and specific bioassays of tumor markers are critical for early cancer detection and treatment.In recent years,lanthanide(Ln3+)doped upconversion nanoparticles(UCNPs)have attracted wide attentions in tumor markers detectio n.Herein,we co mbined NaYF4:Yb,Tm and silver nanoparticles,serving as energy donor and receptor,respectively,to form an up-conversion fluorescence based inhibitory tumor marker biosensor system.The tumor marker CA-125 molecules are labeled with silver NPs,and the energy transfer fluorescent signal can be detected between the UCNPs and the silver NP receptors.The biosensor shows good stability,high sensitivity and selectivity in the tumor marker concentration range from 5 to 100 ng/mL,as well as a detection limit of 120 pg/mL.Due to the advantage of ease of fabrication and operation,low cost and high information capacity,this technology holds great potential for the clinical applications.展开更多
We described a method for the rapid synthesis of uni- form silver nanoparticles （NPs） under microwave heating, using poly（ethylene glycol） （PEG） as reducing agent and solvent and poly（vinylpyrrolidinone） （PVP...We described a method for the rapid synthesis of uni- form silver nanoparticles （NPs） under microwave heating, using poly（ethylene glycol） （PEG） as reducing agent and solvent and poly（vinylpyrrolidinone） （PVP） as capping agent. The transmis- sion electron microscopy （TEM） and UV-Vis extinction spectra were used to characterize the size and uniformity of the silver NPs. At the same reaction temperature, the products with microwave heating were more uniform than that with oil heating. We also investigated the influence of reaction temperature, microwave power, and molar ratio of PVP to AgNO3. In our experiments, uniform silver NPs with mean diameter of 50 nm were synthesized at 140 ℃ under 700 W microwave irradiation with PVP/AgNO3 molar ratio of 5 ： 1.展开更多
基金Project supported by the National Key Research and Development Program(2016YFC0207101)the National Natural Science Foundation of China(61775080,81602659,11704056)the Natural Science Foundation of Jilin Province,China(20170101170JC)
文摘Sensitive and specific bioassays of tumor markers are critical for early cancer detection and treatment.In recent years,lanthanide(Ln3+)doped upconversion nanoparticles(UCNPs)have attracted wide attentions in tumor markers detectio n.Herein,we co mbined NaYF4:Yb,Tm and silver nanoparticles,serving as energy donor and receptor,respectively,to form an up-conversion fluorescence based inhibitory tumor marker biosensor system.The tumor marker CA-125 molecules are labeled with silver NPs,and the energy transfer fluorescent signal can be detected between the UCNPs and the silver NP receptors.The biosensor shows good stability,high sensitivity and selectivity in the tumor marker concentration range from 5 to 100 ng/mL,as well as a detection limit of 120 pg/mL.Due to the advantage of ease of fabrication and operation,low cost and high information capacity,this technology holds great potential for the clinical applications.
基金Supported by the National Natural Science Foundation of China(61008043)the Specialized Research Fund for the Doctoral Program of Higher Education of China(20100141120041)
文摘We described a method for the rapid synthesis of uni- form silver nanoparticles （NPs） under microwave heating, using poly（ethylene glycol） （PEG） as reducing agent and solvent and poly（vinylpyrrolidinone） （PVP） as capping agent. The transmis- sion electron microscopy （TEM） and UV-Vis extinction spectra were used to characterize the size and uniformity of the silver NPs. At the same reaction temperature, the products with microwave heating were more uniform than that with oil heating. We also investigated the influence of reaction temperature, microwave power, and molar ratio of PVP to AgNO3. In our experiments, uniform silver NPs with mean diameter of 50 nm were synthesized at 140 ℃ under 700 W microwave irradiation with PVP/AgNO3 molar ratio of 5 ： 1.
