AIM:To investigate the correlation between gastric cancer growth and signal transducer and activator of transcription-3(STAT3) expression.METHODS:We assessed the expressions of STAT3,phosphor-STAT3(pSTAT3),suppressor ...AIM:To investigate the correlation between gastric cancer growth and signal transducer and activator of transcription-3(STAT3) expression.METHODS:We assessed the expressions of STAT3,phosphor-STAT3(pSTAT3),suppressor of cytokine signaling-1(SOCS-1),survivin and Bcl-2 in gastric cancer patients after gastrectomy by immunohistochemical method.In addition,in situ hybridization was used to further demonstrate the mRNA expression of STAT3 in gastric cancer.RESULTS:With the univariate analysis,expressions of STAT3,pSTAT3,SOCS-1,survivin and Bcl-2,the size of primary tumor and the lymph node metastasis were found to be associated with the overall survival(OS) of gastric cancer patients.However,only pSTAT3 expression and the lymph node metastasis were identified as the independent factors of OS of gastric cancer with multivariate analysis.STAT3 expression was correlated with the lymph node metastasis.There were positive correlations between expressions of STAT3,survivin,Bcl-2 and pSTAT3 in gastric cancer,whereas there was negative correlation between STAT3 expression and SOCS-1 expression in gastric cancer.CONCLUSION:STAT3 can transform into pSTAT3 to promote the survival and inhibit the apoptosis of gastric cancer cells.SOCS-1 might be the valid molecular antagonist to inhibit the STAT3 expression in gastric cancer.展开更多
目的探讨信号转导和转录激活因子-3(signal transducer and activator of transcription-3,STAT3)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与重庆地区汉族儿童川崎病(kawasaki disease,KD)易感性的关系。方法用序列特...目的探讨信号转导和转录激活因子-3(signal transducer and activator of transcription-3,STAT3)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与重庆地区汉族儿童川崎病(kawasaki disease,KD)易感性的关系。方法用序列特异性引物聚合酶链反应(sequence specific primers-polymerase chain reaction,SSP-PCR)分析130例重庆地区汉族川崎病患儿及147名健康儿童STAT3基因内含子11(rs2293152C/G和rs957970C/T)的SNP,统计学分析病例组与对照组之间的差异。结果川崎病组STAT3基因rs2293152C/G位点CC基因型频率(23.8%)显著高于对照组(10.2%),川崎病组rs2293152C等位基因分布频率(45.4%)显著高于对照组(34.0%,P=0.006,OR=1.612,95%CI:1.144～2.272);而STAT3 rs957970C/T位点基因型及等位基因频率分布在川崎病组和对照组间无统计学差异(P>0.05)。结论重庆地区汉族儿童的STAT3基因内含子11中rs2293152位点多态性与川崎病易感性相关,携带rs2293152C等位基因者比G等位基因携带者易罹患川崎病,而STAT3 rs957970C/T位点多态性与该地区儿童川崎病的易感性无显著相关性。展开更多
基金Supported by National Basic Research Program of China(973 Program),No.2010CB529301Tianjin Health Bureau Research Foundation,No.09KC74
文摘AIM:To investigate the correlation between gastric cancer growth and signal transducer and activator of transcription-3(STAT3) expression.METHODS:We assessed the expressions of STAT3,phosphor-STAT3(pSTAT3),suppressor of cytokine signaling-1(SOCS-1),survivin and Bcl-2 in gastric cancer patients after gastrectomy by immunohistochemical method.In addition,in situ hybridization was used to further demonstrate the mRNA expression of STAT3 in gastric cancer.RESULTS:With the univariate analysis,expressions of STAT3,pSTAT3,SOCS-1,survivin and Bcl-2,the size of primary tumor and the lymph node metastasis were found to be associated with the overall survival(OS) of gastric cancer patients.However,only pSTAT3 expression and the lymph node metastasis were identified as the independent factors of OS of gastric cancer with multivariate analysis.STAT3 expression was correlated with the lymph node metastasis.There were positive correlations between expressions of STAT3,survivin,Bcl-2 and pSTAT3 in gastric cancer,whereas there was negative correlation between STAT3 expression and SOCS-1 expression in gastric cancer.CONCLUSION:STAT3 can transform into pSTAT3 to promote the survival and inhibit the apoptosis of gastric cancer cells.SOCS-1 might be the valid molecular antagonist to inhibit the STAT3 expression in gastric cancer.
文摘目的探讨信号转导和转录激活因子-3(signal transducer and activator of transcription-3,STAT3)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与重庆地区汉族儿童川崎病(kawasaki disease,KD)易感性的关系。方法用序列特异性引物聚合酶链反应(sequence specific primers-polymerase chain reaction,SSP-PCR)分析130例重庆地区汉族川崎病患儿及147名健康儿童STAT3基因内含子11(rs2293152C/G和rs957970C/T)的SNP,统计学分析病例组与对照组之间的差异。结果川崎病组STAT3基因rs2293152C/G位点CC基因型频率(23.8%)显著高于对照组(10.2%),川崎病组rs2293152C等位基因分布频率(45.4%)显著高于对照组(34.0%,P=0.006,OR=1.612,95%CI:1.144～2.272);而STAT3 rs957970C/T位点基因型及等位基因频率分布在川崎病组和对照组间无统计学差异(P>0.05)。结论重庆地区汉族儿童的STAT3基因内含子11中rs2293152位点多态性与川崎病易感性相关,携带rs2293152C等位基因者比G等位基因携带者易罹患川崎病,而STAT3 rs957970C/T位点多态性与该地区儿童川崎病的易感性无显著相关性。
