目的：分析重度溶血对人血清生化项目检测结果的干扰及消除干扰的方法。方法选取2013年1～12月钟山县人民医院静脉血样本150份，受检者抽取5 mL 静脉血液，将样本平分到洁净干燥试管中，一支为观察组，另一支为对照组。采用东芝 TBA‐12...目的：分析重度溶血对人血清生化项目检测结果的干扰及消除干扰的方法。方法选取2013年1～12月钟山县人民医院静脉血样本150份，受检者抽取5 mL 静脉血液，将样本平分到洁净干燥试管中，一支为观察组，另一支为对照组。采用东芝 TBA‐120全自动生化分析仪，检测试剂采用北京利德曼试剂盒，仪器操作和试剂盒操作均按照说明书及操作规范进行。观察组样本为溶血血清，对照组为正常血清，采用高速离心机对样本进行离心处理，离心时间5 min ，转速为3000 r／min ，然后由同一组操作人员进行上机操作检测。检测项目包括血液中血糖、碱性磷酸酶、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、总蛋白、尿酸、清蛋白生化项目检测指标。结果观察组溶血样本的血糖、碱性磷酸酶的指标低于对照组样本；观察组溶血样本的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、尿酸、清蛋白的指标均高于对照组样本。结论溶血现象会影响临床血清检验项目的结果，并对医生诊断及治疗效果评价产生影响，在血液样本采集后应积极预防溶血现象，确保血清检测结果的准确性。展开更多
Background Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was...Background Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was to develop an RNAi (RNA interference) approach that specifically targeted the N gene sequence of severe acute respiratory syndrome associated coronavirus (SARS-CoV) by synthesizing short hairpin RNA (shRNA) in vivo , and to assess the inhibitory effect of this shRNA on SARS-CoV N antigen expression. Methods The eukaryotic expression plasmid pEGFP-C1-N, containing SARS-CoV N gene, was co-transfected into 293 cells with either the RNAi plasmid pshRNA-N or unrelated control plasmid pshRNA-HBV-C4. At 24, 48 and 72 hours post transfection, the green fluorescence was observed through a fluorescence microscope. The RNA levels of SARS-CoV N were determined by reverse transcription polymerase chain reaction (RT-PCR). The expression of Green Fluorescent Protein (GFP) and protein N were detected using Western blot.Results The vector, pshRNA-N expressing shRNA which targeted the N gene of SARS-CoV, was successfully constructed. The introduction of RNAi plasmid efficiently and specifically inhibited the synthesis of protein N. RT-PCR showed that RNAs of N gene were clearly reduced when the pEGFP-C1-N was cotransfected with pshRNA-N, whereas the control vector did not exhibit inhibitory effect on N gene transcription.Conclusions Our results demonstrate that RNAi mediated silencing of SARS-CoV gene could effectively inhibit expression of SARS-CoV antigen, hence RNAi based strategy should be further explored as a more efficacious antiviral therapy of SARS-CoV infection.展开更多
文摘目的：分析重度溶血对人血清生化项目检测结果的干扰及消除干扰的方法。方法选取2013年1～12月钟山县人民医院静脉血样本150份，受检者抽取5 mL 静脉血液，将样本平分到洁净干燥试管中，一支为观察组，另一支为对照组。采用东芝 TBA‐120全自动生化分析仪，检测试剂采用北京利德曼试剂盒，仪器操作和试剂盒操作均按照说明书及操作规范进行。观察组样本为溶血血清，对照组为正常血清，采用高速离心机对样本进行离心处理，离心时间5 min ，转速为3000 r／min ，然后由同一组操作人员进行上机操作检测。检测项目包括血液中血糖、碱性磷酸酶、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、总蛋白、尿酸、清蛋白生化项目检测指标。结果观察组溶血样本的血糖、碱性磷酸酶的指标低于对照组样本；观察组溶血样本的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、尿酸、清蛋白的指标均高于对照组样本。结论溶血现象会影响临床血清检验项目的结果，并对医生诊断及治疗效果评价产生影响，在血液样本采集后应积极预防溶血现象，确保血清检测结果的准确性。
基金ThisworkwassupportedbythegrantsfromNationalScienceFundforDistinguishedYoungScholars(No 30228026 ) andNational863 ProgramintheTenthFive YearPlanPeriod (No 2001AA217121)
文摘Background Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was to develop an RNAi (RNA interference) approach that specifically targeted the N gene sequence of severe acute respiratory syndrome associated coronavirus (SARS-CoV) by synthesizing short hairpin RNA (shRNA) in vivo , and to assess the inhibitory effect of this shRNA on SARS-CoV N antigen expression. Methods The eukaryotic expression plasmid pEGFP-C1-N, containing SARS-CoV N gene, was co-transfected into 293 cells with either the RNAi plasmid pshRNA-N or unrelated control plasmid pshRNA-HBV-C4. At 24, 48 and 72 hours post transfection, the green fluorescence was observed through a fluorescence microscope. The RNA levels of SARS-CoV N were determined by reverse transcription polymerase chain reaction (RT-PCR). The expression of Green Fluorescent Protein (GFP) and protein N were detected using Western blot.Results The vector, pshRNA-N expressing shRNA which targeted the N gene of SARS-CoV, was successfully constructed. The introduction of RNAi plasmid efficiently and specifically inhibited the synthesis of protein N. RT-PCR showed that RNAs of N gene were clearly reduced when the pEGFP-C1-N was cotransfected with pshRNA-N, whereas the control vector did not exhibit inhibitory effect on N gene transcription.Conclusions Our results demonstrate that RNAi mediated silencing of SARS-CoV gene could effectively inhibit expression of SARS-CoV antigen, hence RNAi based strategy should be further explored as a more efficacious antiviral therapy of SARS-CoV infection.
