<p style="text-align:justify;"> <span><i><span style="font-family:""><span style="font-family:Verdana;">Citrus </span><span style="font-fa...<p style="text-align:justify;"> <span><i><span style="font-family:""><span style="font-family:Verdana;">Citrus </span><span style="font-family:Verdana;">tristeza</span><span style="font-family:Verdana;"> virus</span></span></i></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> (CTV) outbreaks have been reported in the main citrus growing region of Mexico in the past four years. Recently, in eastern Mexico (the major citrus-growing region in the country), severe CTV isolates have been detected. However, the molecular identity of </span><span style="font-family:Verdana;">observed</span><span style="font-family:Verdana;"> isolates remains unestablished. This research was undertaken to elucidate the molecular characterization of CTV populations spreading in this region and to compare it with </span><span style="font-family:Verdana;">phylogeny</span><span style="font-family:Verdana;"> of existing isolates. Genotyping of 32 collected isolates was performed using reverse-transcription polymerase chain reaction (RT-PCR) with sequence analysis of the coat protein (CP) gene, putatively associated with pathogenicity. This protein is a 25 kDa major capsid protein, which forms a long virion body coating 95% of the particle length. </span><span style="font-family:Verdana;">A comparative</span><span style="font-family:Verdana;"> sequence </span><span style="font-family:Verdana;">analys</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">is</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> was performed using CTV sequences from different geographical origins already published and deposited in the GenBank databases. Phylogenetic analysis showed that the degree of sequence divergence among isolates correlated with their pathogenicity. Based on the sequencing results, the collected isolates were categorizedn as mild or severe phylogenetic clusters展开更多
文摘<p style="text-align:justify;"> <span><i><span style="font-family:""><span style="font-family:Verdana;">Citrus </span><span style="font-family:Verdana;">tristeza</span><span style="font-family:Verdana;"> virus</span></span></i></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> (CTV) outbreaks have been reported in the main citrus growing region of Mexico in the past four years. Recently, in eastern Mexico (the major citrus-growing region in the country), severe CTV isolates have been detected. However, the molecular identity of </span><span style="font-family:Verdana;">observed</span><span style="font-family:Verdana;"> isolates remains unestablished. This research was undertaken to elucidate the molecular characterization of CTV populations spreading in this region and to compare it with </span><span style="font-family:Verdana;">phylogeny</span><span style="font-family:Verdana;"> of existing isolates. Genotyping of 32 collected isolates was performed using reverse-transcription polymerase chain reaction (RT-PCR) with sequence analysis of the coat protein (CP) gene, putatively associated with pathogenicity. This protein is a 25 kDa major capsid protein, which forms a long virion body coating 95% of the particle length. </span><span style="font-family:Verdana;">A comparative</span><span style="font-family:Verdana;"> sequence </span><span style="font-family:Verdana;">analys</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">is</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> was performed using CTV sequences from different geographical origins already published and deposited in the GenBank databases. Phylogenetic analysis showed that the degree of sequence divergence among isolates correlated with their pathogenicity. Based on the sequencing results, the collected isolates were categorizedn as mild or severe phylogenetic clusters
