Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six gro...Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide （Cy） model group, Levamisole （positive control） group, RIP low, medium and high dose groups （0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively）, ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin （HC50）, proliferation of lymphocytes and expression of related cytokines, interleukin （IL-2） and interferon γ, （INF-γ）, the effect of RIP on immunological function and its mechanism were studied. Results： RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11 ±0.13 to 1.42±0.26. The level of IL-2 and INF- γ, could be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF- γ, to 53.54 ± 14.43 ng/L and 189.91 ± 32.63 ng/L, respectively. Conclusion： RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.展开更多
目的探讨蓝芩口服液对慢性咽炎患者血清炎症细胞因子、免疫细胞亚群及临床症状的影响。方法2组均进行常规治疗,对照组在此基础上口服喉疾灵片3粒/次,3次/d,观察组口服蓝芩口服液20 m L/次,3次/d,2组患者均7 d为1个疗程,连用3个疗程,检...目的探讨蓝芩口服液对慢性咽炎患者血清炎症细胞因子、免疫细胞亚群及临床症状的影响。方法2组均进行常规治疗,对照组在此基础上口服喉疾灵片3粒/次,3次/d,观察组口服蓝芩口服液20 m L/次,3次/d,2组患者均7 d为1个疗程,连用3个疗程,检测治疗前后血清白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)水平,外周血T淋巴细胞亚群及B细胞数,观察2组临床症状改善情况及不良反应情况。结果治疗后2组血清IL-2、TNF-α水平较治疗前改善,改善程度观察组优于对照组,差异有统计学意义(P<0.05);治疗后CD3、CD4、CD4/CD8及B细胞数均较治疗前改善,且观察组优于对照组,差异具有统计学意义(P<0.05);观察组咽喉部疼痛、异物感、干痒、咳嗽等改善时间明显短于对照组,差异具有统计学意义(P<0.05);观察组有2例轻度腹泻,停药后自行缓解,对照组未见明显不良反应。结论蓝芩口服液有较好的抗炎、抗病毒及增强免疫作用,可以有效缓解慢性咽炎症状。展开更多
AIM To investigate the value of multiparameter joint analysis in the early diagnosis of gastric cancer(GC) in clinical practice.METHODS Concentrations of CEA, CA724 and three kinds of cytokines(TNF-α, IL-6 and IL-8) ...AIM To investigate the value of multiparameter joint analysis in the early diagnosis of gastric cancer(GC) in clinical practice.METHODS Concentrations of CEA, CA724 and three kinds of cytokines(TNF-α, IL-6 and IL-8) in 176 GC patients, 117 atypical hyperplasia patients, and 204 healthy control individuals were used for building the diagnostic model, then 58 GC patients, 41 atypical hyperplasia patients, and 66 healthy control individuals were enrolled independently. The joints of the indicators were analyzed by binary logistic regression analysis method.RESULTS For discriminating the healthy control group and the GC group, IL-6 had the best diagnostic value, and the area under curve(AUC) of joint analysis was 0.95(0.93-0.97). For the early stage and advanced stage GC, the AUC were 0.95(0.92-0.98) and 0.95(0.92-0.97). For discriminating the atypical hyperplasia group and GC group, CA724 had the best diagnostic value, and the AUC of joint analysis was 0.97(0.95-0.99). For the early stage and advanced stage GC groups, the AUC were 0.98(0.96-0.99) and 0.96(0.94-0.98). After evaluation, for discriminating the GC, early stage GC and advanced cancer group from the healthy control group, the diagnostic sensitivity was 89.66%, 84.21% and 92.31%, respectively, and the specificity was 92.42%, 90.91% and 90.91%. For discriminating the GC, early stage GC and advanced cancer groups from the atypical hyperplasia group, the diagnostic sensitivity was 87.93%, 78.95% and 92.31%, respectively, and the specificity was 87.80%, 85.37% and 90.24%.CONCLUSION We have built a diagnostic model including CEA, CA724, IL-6, IL-8, and TNF-α. It may provide potential assistance as a screening method for the early detection of GC.展开更多
基金Foundation of State Administration of Traditional Chinese Medicine(No.04-05ZP970)Military 11th Five-Year Foundation(No.06MA360)
文摘Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide （Cy） model group, Levamisole （positive control） group, RIP low, medium and high dose groups （0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively）, ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin （HC50）, proliferation of lymphocytes and expression of related cytokines, interleukin （IL-2） and interferon γ, （INF-γ）, the effect of RIP on immunological function and its mechanism were studied. Results： RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11 ±0.13 to 1.42±0.26. The level of IL-2 and INF- γ, could be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF- γ, to 53.54 ± 14.43 ng/L and 189.91 ± 32.63 ng/L, respectively. Conclusion： RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.
文摘目的探讨蓝芩口服液对慢性咽炎患者血清炎症细胞因子、免疫细胞亚群及临床症状的影响。方法2组均进行常规治疗,对照组在此基础上口服喉疾灵片3粒/次,3次/d,观察组口服蓝芩口服液20 m L/次,3次/d,2组患者均7 d为1个疗程,连用3个疗程,检测治疗前后血清白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)水平,外周血T淋巴细胞亚群及B细胞数,观察2组临床症状改善情况及不良反应情况。结果治疗后2组血清IL-2、TNF-α水平较治疗前改善,改善程度观察组优于对照组,差异有统计学意义(P<0.05);治疗后CD3、CD4、CD4/CD8及B细胞数均较治疗前改善,且观察组优于对照组,差异具有统计学意义(P<0.05);观察组咽喉部疼痛、异物感、干痒、咳嗽等改善时间明显短于对照组,差异具有统计学意义(P<0.05);观察组有2例轻度腹泻,停药后自行缓解,对照组未见明显不良反应。结论蓝芩口服液有较好的抗炎、抗病毒及增强免疫作用,可以有效缓解慢性咽炎症状。
基金Supported by Henan Province science and Technology Research Projects,No.162102310041National Key R&D Program of China,No.2016YFC0106604National Natural Science Foundation of China,No.81502591
文摘AIM To investigate the value of multiparameter joint analysis in the early diagnosis of gastric cancer(GC) in clinical practice.METHODS Concentrations of CEA, CA724 and three kinds of cytokines(TNF-α, IL-6 and IL-8) in 176 GC patients, 117 atypical hyperplasia patients, and 204 healthy control individuals were used for building the diagnostic model, then 58 GC patients, 41 atypical hyperplasia patients, and 66 healthy control individuals were enrolled independently. The joints of the indicators were analyzed by binary logistic regression analysis method.RESULTS For discriminating the healthy control group and the GC group, IL-6 had the best diagnostic value, and the area under curve(AUC) of joint analysis was 0.95(0.93-0.97). For the early stage and advanced stage GC, the AUC were 0.95(0.92-0.98) and 0.95(0.92-0.97). For discriminating the atypical hyperplasia group and GC group, CA724 had the best diagnostic value, and the AUC of joint analysis was 0.97(0.95-0.99). For the early stage and advanced stage GC groups, the AUC were 0.98(0.96-0.99) and 0.96(0.94-0.98). After evaluation, for discriminating the GC, early stage GC and advanced cancer group from the healthy control group, the diagnostic sensitivity was 89.66%, 84.21% and 92.31%, respectively, and the specificity was 92.42%, 90.91% and 90.91%. For discriminating the GC, early stage GC and advanced cancer groups from the atypical hyperplasia group, the diagnostic sensitivity was 87.93%, 78.95% and 92.31%, respectively, and the specificity was 87.80%, 85.37% and 90.24%.CONCLUSION We have built a diagnostic model including CEA, CA724, IL-6, IL-8, and TNF-α. It may provide potential assistance as a screening method for the early detection of GC.
