Interferon （IFN）-αs bind to and activate their cognate cell surface receptor to invoke an antiviral response in target cells. Well-described receptor-mediated signaling events result in transcriptional regulation o...Interferon （IFN）-αs bind to and activate their cognate cell surface receptor to invoke an antiviral response in target cells. Well-described receptor-mediated signaling events result in transcriptional regulation of IFN sensitive genes, effectors of this antiviral response. Results from a pilot study to evaluate the clinical efficacy of IFN-α treatment of SARS patients provided evidence for IFN-inducible resolution of disease. In this report we examined the contribution of IFN-inducible phosphorylation-activation of specific signaling effectors to protection from infection by a SARS-related murine coronavirus, MHV-1. As anticipated, the earliest receptor-activation event, Jakl phosphorylation, is critical for IFN-inducible protection from MHV-1 infection. Additionally, we provide evidence for the contribution of two kinases, the MAP kinase p38MAPK, and protein kinase C （PKC） 5 to antiviral protection from MHV-1 infection. Notably, our data suggest that MHV^I infection, as for the Urbani SARS coronoavirus, inhibits an IFN response, inferred from the lack of activation ofpkr and 2 ＇5 ＇-oas, genes associated with mediating the antiviral activities of IFN-as. To identify potential target genes that are activated downstream of the IFN-inducible signaling effectors we identified, and that mediate protection from coronavirus infection, we examined the gene expression profiles in the peripheral blood mononuclear cells of SARS patients who received IFN treatment. A subset of differentially regulated genes were distinguished with functional properties associated with antimicrobial activities.展开更多
[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was ...[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by BLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of B. juncea.展开更多
The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation.Two populations were a...The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation.Two populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.展开更多
目的研究深圳市南山区2009 2015年医院内患者临床检测标本、医院台面与医护人员双手涂抹样以及食物中毒样本中金黄色葡萄球菌(Staphylococcus aureus,SA)蛋白A基因多态性分型(single locus DNA-sequencing of the repeat region of the ...目的研究深圳市南山区2009 2015年医院内患者临床检测标本、医院台面与医护人员双手涂抹样以及食物中毒样本中金黄色葡萄球菌(Staphylococcus aureus,SA)蛋白A基因多态性分型(single locus DNA-sequencing of the repeat region of the Staphylococcus protein A gene,Spa)的特征及毒力基因分布情况。方法采用多重PCR方法检测SA的mec A与fem A基因,PCR方法检测24个毒力基因以及SA蛋白A基因,PCR产物测序后序列上传数据库进行比对。结果 87株SA共检出48株耐甲氧西林金黄色葡萄球菌(methicillin-resistant S.aureus,MRSA),检出率为55.2%。共检出21种毒力基因,检出率>60%的毒力基因有LUKDE(65.5%)、SHE(67.8%)、mpHLG2(85.1%)、HLD(93.1%)和HLA(100.0%)。94.3%菌株携带5个以上毒力基因。三类样本中有11种毒力基因检出率差异有统计学意义,分别是SEB、mpSEC、mpSEJ、mpSEO、TST、HLB、HLD、mpHLG1、mpHLG2,mpETB和PVL。其中mpETB与PVL仅在患者中检出,肠毒素基因在食物中毒株中携带率较高。MRSA与甲氧西林敏感金黄色葡萄球菌(methicillin-sensitive S.aureus,MSSA)的3种毒力基因检出率差异有统计学意义,分别是mpETA、PVL及mpHLG2;MRSA的毒力基因mpETA与PVL检出率明显高于MSSA(P=0.033,P=0.030),而mpHLG2检出率明显低于MSSA(P=0.021)。87株SA分为24种Spa型,患者优势型别为t030(30.0%)与t437(36.7%),医院其他样本优势型别为t091(50.0%),食物中毒优势型别为t127(36.8%)与t091(21.1%)。结论三类样本部分毒力基因检出差异有统计学意义。MRSA与MSSA携带毒力基因差异无统计学意义。三类样本中均存在优势的Spa型别。展开更多
Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this stu...Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this study, with methylation-sensitive amplification polymorphism （MSAP） technique, DNA methylation was compared between the leaves inoculated with Foc TR4 and the mock-inoculated leaves at different pathogenic stages. With 25 pairs of primers, 1 144 and 1 255 fragments were amplified from the infected and mock-inoculated leaves, respectively. DNA methylation was both changed and the average methylated CCGG sequences were 34.81 and 29.26% for the infected and the mock-inoculated leaves. And DNA hypermethylation and hypomethylation were induced by pathogen infection during all pathogenic stages. Further, 69 polymorphic fragments were sequenced and 29 of them showed sequence similarity to genes with known functions. And RT-PCR results of four genes indicated that their expression patterns were consistent with their methylation patterns. Our results suggest that DNA methylation plays important roles in pathogenic response to Foc TR4 for banana.展开更多
文摘Interferon （IFN）-αs bind to and activate their cognate cell surface receptor to invoke an antiviral response in target cells. Well-described receptor-mediated signaling events result in transcriptional regulation of IFN sensitive genes, effectors of this antiviral response. Results from a pilot study to evaluate the clinical efficacy of IFN-α treatment of SARS patients provided evidence for IFN-inducible resolution of disease. In this report we examined the contribution of IFN-inducible phosphorylation-activation of specific signaling effectors to protection from infection by a SARS-related murine coronavirus, MHV-1. As anticipated, the earliest receptor-activation event, Jakl phosphorylation, is critical for IFN-inducible protection from MHV-1 infection. Additionally, we provide evidence for the contribution of two kinases, the MAP kinase p38MAPK, and protein kinase C （PKC） 5 to antiviral protection from MHV-1 infection. Notably, our data suggest that MHV^I infection, as for the Urbani SARS coronoavirus, inhibits an IFN response, inferred from the lack of activation ofpkr and 2 ＇5 ＇-oas, genes associated with mediating the antiviral activities of IFN-as. To identify potential target genes that are activated downstream of the IFN-inducible signaling effectors we identified, and that mediate protection from coronavirus infection, we examined the gene expression profiles in the peripheral blood mononuclear cells of SARS patients who received IFN treatment. A subset of differentially regulated genes were distinguished with functional properties associated with antimicrobial activities.
基金Supported by National Natural Science Foundation of China(31160289)Rapeseed Industry Construction Program of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by BLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of B. juncea.
文摘The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation.Two populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.
文摘目的研究深圳市南山区2009 2015年医院内患者临床检测标本、医院台面与医护人员双手涂抹样以及食物中毒样本中金黄色葡萄球菌(Staphylococcus aureus,SA)蛋白A基因多态性分型(single locus DNA-sequencing of the repeat region of the Staphylococcus protein A gene,Spa)的特征及毒力基因分布情况。方法采用多重PCR方法检测SA的mec A与fem A基因,PCR方法检测24个毒力基因以及SA蛋白A基因,PCR产物测序后序列上传数据库进行比对。结果 87株SA共检出48株耐甲氧西林金黄色葡萄球菌(methicillin-resistant S.aureus,MRSA),检出率为55.2%。共检出21种毒力基因,检出率>60%的毒力基因有LUKDE(65.5%)、SHE(67.8%)、mpHLG2(85.1%)、HLD(93.1%)和HLA(100.0%)。94.3%菌株携带5个以上毒力基因。三类样本中有11种毒力基因检出率差异有统计学意义,分别是SEB、mpSEC、mpSEJ、mpSEO、TST、HLB、HLD、mpHLG1、mpHLG2,mpETB和PVL。其中mpETB与PVL仅在患者中检出,肠毒素基因在食物中毒株中携带率较高。MRSA与甲氧西林敏感金黄色葡萄球菌(methicillin-sensitive S.aureus,MSSA)的3种毒力基因检出率差异有统计学意义,分别是mpETA、PVL及mpHLG2;MRSA的毒力基因mpETA与PVL检出率明显高于MSSA(P=0.033,P=0.030),而mpHLG2检出率明显低于MSSA(P=0.021)。87株SA分为24种Spa型,患者优势型别为t030(30.0%)与t437(36.7%),医院其他样本优势型别为t091(50.0%),食物中毒优势型别为t127(36.8%)与t091(21.1%)。结论三类样本部分毒力基因检出差异有统计学意义。MRSA与MSSA携带毒力基因差异无统计学意义。三类样本中均存在优势的Spa型别。
基金supported by the National Natural Science Foundation of China (30860149 and 31360364)the Joint Support Program from Tropical Crop Breeding Engineering Center of Ministry of Education of China+1 种基金the Crop Science National Key Disciplines of China (lhxm-2012-2)the Key Scientific Research Program from Hainan Province,China (ZDZX2013023)
文摘Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this study, with methylation-sensitive amplification polymorphism （MSAP） technique, DNA methylation was compared between the leaves inoculated with Foc TR4 and the mock-inoculated leaves at different pathogenic stages. With 25 pairs of primers, 1 144 and 1 255 fragments were amplified from the infected and mock-inoculated leaves, respectively. DNA methylation was both changed and the average methylated CCGG sequences were 34.81 and 29.26% for the infected and the mock-inoculated leaves. And DNA hypermethylation and hypomethylation were induced by pathogen infection during all pathogenic stages. Further, 69 polymorphic fragments were sequenced and 29 of them showed sequence similarity to genes with known functions. And RT-PCR results of four genes indicated that their expression patterns were consistent with their methylation patterns. Our results suggest that DNA methylation plays important roles in pathogenic response to Foc TR4 for banana.
