本研究旨在建立一种快速构建猪圆环病毒2型(porcine circovirus type 2,PCV2)感染性克隆的方法。以临床分离的PCV2 LX株为模板,利用DNA无缝克隆技术成功构建了PCV2环状感染性克隆。同时,在基因组体外自连接的构建过程以及拯救病毒的生...本研究旨在建立一种快速构建猪圆环病毒2型(porcine circovirus type 2,PCV2)感染性克隆的方法。以临床分离的PCV2 LX株为模板,利用DNA无缝克隆技术成功构建了PCV2环状感染性克隆。同时,在基因组体外自连接的构建过程以及拯救病毒的生长特性上,将本方法与常规酶切连接方法进行了比较。本方法无须分析和引入限制性内切酶位点,避免了传统酶切连接的繁琐,能更高效地进行PCV2基因组的编辑。构建的感染性克隆经脂质体转染细胞,成功拯救获得可以稳定传代的重组病毒,并且重组病毒在PK-15细胞和3D4/31细胞(猪肺泡巨噬细胞系)上具有更强的增殖能力。综上所述,本研究构建了一种基于体外自连接的PCV2反向遗传操作系统,为PCV2基因工程疫苗的研发提供新策略,为其他新发圆环病毒(PCV3和PCV4)感染性克隆的构建提供借鉴。展开更多
目的运用三维有限元分析方法,分析传统托槽与被动自锁托槽扭转性能的差异。方法建立HX(国产传统系列)、MUT(3M Mini Uni Twin传统系列)、3M(3M被动自锁系列)、Damon Q(Ormco被动自锁系列)托槽及相应弓丝的三维有限元模型,模型装配后进...目的运用三维有限元分析方法,分析传统托槽与被动自锁托槽扭转性能的差异。方法建立HX(国产传统系列)、MUT(3M Mini Uni Twin传统系列)、3M(3M被动自锁系列)、Damon Q(Ormco被动自锁系列)托槽及相应弓丝的三维有限元模型,模型装配后进行网格划分,参数设定,边界限定及力值加载,最后绘制力矩值图形进行分析。结果托槽相同时,扭转力矩值随弓丝尺寸增加而增加。弓丝尺寸相同时,HX托槽所得扭转力矩值最大,其次分别为MUT、3M、Damon Q。结论托槽的扭转性能与槽沟长度、结扎方式、弓丝尺寸等关系密切。传统结扎方式托槽扭转性能明显高于被动自锁托槽,且Damon Q托槽的扭转性能最差。展开更多
The aim of this study was to investigate the amount of external apical root resorption (EARR) and the release of interleukin (IL)-6 in the gingival crevicular fluid (GCF) in subjects treated with a low-force low-frict...The aim of this study was to investigate the amount of external apical root resorption (EARR) and the release of interleukin (IL)-6 in the gingival crevicular fluid (GCF) in subjects treated with a low-force low-friction system. Sixty patients were assigned to two groups of thirty patients for each: one group received treatment with self-ligating brackets and the other with conventional ligated edgewise brackets. All patients were treated with extraction of the maxillary first premolars. The EARR of the maxillary central incisors was evaluated on the periapical radiographs and cephalograms, taken before and after orthodontic treatment. The GCF was also collected non-invasively from the mesial and distal sides of central incisors by using filter paper strips before and after orthodontic treatment. Enzyme-linked immunosorbent assay (ELISA) kits were used to determine the IL-6 levels in the GCF samples. A significant difference was found in the amount of EARR between the patients with self-ligating brackets and conventional brackets. The mean amount of EARR was significantly lower for self-ligating brackets than conventional brackets (p < 0.05). The GCF levels of IL-6 for the patients with self-ligating brackets appliance were significantly lower than for those with the conventional brackets (p < 0.05). These results show that the mean amount of EARR and the GCF levels of IL-6 were significantly lower in the patients treated using low-force low-friction appliances than conventional brackets. Therefore, self-ligating brackets may be a useful system for reducing inflammation and EARR.展开更多
Objective:To explore the effect of self-ligating bracket appliance on the periodontal tissues in patients with chronic periodontitis after orthodontic treatment.Methods:A total of 56 patients with chronic periodontiti...Objective:To explore the effect of self-ligating bracket appliance on the periodontal tissues in patients with chronic periodontitis after orthodontic treatment.Methods:A total of 56 patients with chronic periodontitis who were admitted in our hospital were included in the study and divided into the observation group and the control group according to different treatment protocols. The patients in the observation group were given self-ligating brackets, while the patients in the control group were given traditional brackets. The periodontal tissues and tooth motility (TM) before and after treatment in the two groups were observed and recorded. IL-1βand TNF-α levels in the gingival crervicular fluid (GCF) before and after treatment in the two groups were detected.Results:PD, CAL, SBI, and PLI after treatment in the two groups were significantly reduced, while GR was significantly elevated when compared with the control group. PD, CAL, SBI, and PLI after treatment in the observation group were significantly lower than those in the control group, while GR was significantly higher than that in the control group. TM after treatment in the two groups was significantly improved, among which the number of 0° TM was significantly increased, while the number ofⅠ°-Ⅲ°TM was significantly reduced. IL-1β and TNF-α levels in GCF after treatment in the two groups were significantly reduced when compared with before treatment. IL-1β and TNF-α levels in GCF after treatment in the observation group were significantly lower than those in the control group.Conclusions:The self-ligating brackets can effectively improve the periodontal tissues and TM in patients with chronic periodontitis, and reduce the levels of inflammatory cytokines in periodontal tissues, with an accurate efficacy.展开更多
文摘本研究旨在建立一种快速构建猪圆环病毒2型(porcine circovirus type 2,PCV2)感染性克隆的方法。以临床分离的PCV2 LX株为模板,利用DNA无缝克隆技术成功构建了PCV2环状感染性克隆。同时,在基因组体外自连接的构建过程以及拯救病毒的生长特性上,将本方法与常规酶切连接方法进行了比较。本方法无须分析和引入限制性内切酶位点,避免了传统酶切连接的繁琐,能更高效地进行PCV2基因组的编辑。构建的感染性克隆经脂质体转染细胞,成功拯救获得可以稳定传代的重组病毒,并且重组病毒在PK-15细胞和3D4/31细胞(猪肺泡巨噬细胞系)上具有更强的增殖能力。综上所述,本研究构建了一种基于体外自连接的PCV2反向遗传操作系统,为PCV2基因工程疫苗的研发提供新策略,为其他新发圆环病毒(PCV3和PCV4)感染性克隆的构建提供借鉴。
文摘目的运用三维有限元分析方法,分析传统托槽与被动自锁托槽扭转性能的差异。方法建立HX(国产传统系列)、MUT(3M Mini Uni Twin传统系列)、3M(3M被动自锁系列)、Damon Q(Ormco被动自锁系列)托槽及相应弓丝的三维有限元模型,模型装配后进行网格划分,参数设定,边界限定及力值加载,最后绘制力矩值图形进行分析。结果托槽相同时,扭转力矩值随弓丝尺寸增加而增加。弓丝尺寸相同时,HX托槽所得扭转力矩值最大,其次分别为MUT、3M、Damon Q。结论托槽的扭转性能与槽沟长度、结扎方式、弓丝尺寸等关系密切。传统结扎方式托槽扭转性能明显高于被动自锁托槽,且Damon Q托槽的扭转性能最差。
文摘The aim of this study was to investigate the amount of external apical root resorption (EARR) and the release of interleukin (IL)-6 in the gingival crevicular fluid (GCF) in subjects treated with a low-force low-friction system. Sixty patients were assigned to two groups of thirty patients for each: one group received treatment with self-ligating brackets and the other with conventional ligated edgewise brackets. All patients were treated with extraction of the maxillary first premolars. The EARR of the maxillary central incisors was evaluated on the periapical radiographs and cephalograms, taken before and after orthodontic treatment. The GCF was also collected non-invasively from the mesial and distal sides of central incisors by using filter paper strips before and after orthodontic treatment. Enzyme-linked immunosorbent assay (ELISA) kits were used to determine the IL-6 levels in the GCF samples. A significant difference was found in the amount of EARR between the patients with self-ligating brackets and conventional brackets. The mean amount of EARR was significantly lower for self-ligating brackets than conventional brackets (p < 0.05). The GCF levels of IL-6 for the patients with self-ligating brackets appliance were significantly lower than for those with the conventional brackets (p < 0.05). These results show that the mean amount of EARR and the GCF levels of IL-6 were significantly lower in the patients treated using low-force low-friction appliances than conventional brackets. Therefore, self-ligating brackets may be a useful system for reducing inflammation and EARR.
文摘Objective:To explore the effect of self-ligating bracket appliance on the periodontal tissues in patients with chronic periodontitis after orthodontic treatment.Methods:A total of 56 patients with chronic periodontitis who were admitted in our hospital were included in the study and divided into the observation group and the control group according to different treatment protocols. The patients in the observation group were given self-ligating brackets, while the patients in the control group were given traditional brackets. The periodontal tissues and tooth motility (TM) before and after treatment in the two groups were observed and recorded. IL-1βand TNF-α levels in the gingival crervicular fluid (GCF) before and after treatment in the two groups were detected.Results:PD, CAL, SBI, and PLI after treatment in the two groups were significantly reduced, while GR was significantly elevated when compared with the control group. PD, CAL, SBI, and PLI after treatment in the observation group were significantly lower than those in the control group, while GR was significantly higher than that in the control group. TM after treatment in the two groups was significantly improved, among which the number of 0° TM was significantly increased, while the number ofⅠ°-Ⅲ°TM was significantly reduced. IL-1β and TNF-α levels in GCF after treatment in the two groups were significantly reduced when compared with before treatment. IL-1β and TNF-α levels in GCF after treatment in the observation group were significantly lower than those in the control group.Conclusions:The self-ligating brackets can effectively improve the periodontal tissues and TM in patients with chronic periodontitis, and reduce the levels of inflammatory cytokines in periodontal tissues, with an accurate efficacy.
