A laboratory incubation experiment (20℃) was conducted to find if the detrimental effects of seed-soaked Cu on wheat seedlings can be minimized by reducing time duration of seed in contact with Cu EDTA fertilizer sol...A laboratory incubation experiment (20℃) was conducted to find if the detrimental effects of seed-soaked Cu on wheat seedlings can be minimized by reducing time duration of seed in contact with Cu EDTA fertilizer solution. The 24 treatments in a 6 x 4 factorial arrangement included 6 rates/amounts of Cu (0, 15, 30, 60, 120 and 240 g Cu 100 kg-1 seed) and 4 seed-soaking time durations (0, 4, 8 and 16 h). The germination of wheat seed was 100% in the zero-Cu control treatments, irrespective of the duration of seed soaking time. However, seed germination decreased with increasing amount of fertilizer Cu in the seed-soaking solution, and the magnitude of reduction in seed germination due to Cu toxicity increased with increasing duration of seed-soaking time in the Cu fertilizer solution. For the seed-soaked treatments, the detrimental effect of Cu on germination was greatest with 16 h soaking, where only 13% - 18% of the seeds germinated with Cu applied at 15 to 30 g Cu 100 kg-1 seed. For the 4 and 8 h seed soaking treatments, germination of wheat seed ranged from 73% to 83% with 15 g Cu 100 kg-1 seed treatment and 42% to 62% with 30 g Cu 100 kg-1 seed. The findings suggest that the detrimental effect of Cu on germination of wheat seed soaked in Cu EDTA solution can be decreased by reducing duration of soaking time from 16 h to 4 or 8 h, but this needs further investigation using soil under growth chamber and/or field conditions in order to make valid recommendations for use of this new technology on a commercial scale.展开更多
以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水...以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon指数、Chao1指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。展开更多
文摘A laboratory incubation experiment (20℃) was conducted to find if the detrimental effects of seed-soaked Cu on wheat seedlings can be minimized by reducing time duration of seed in contact with Cu EDTA fertilizer solution. The 24 treatments in a 6 x 4 factorial arrangement included 6 rates/amounts of Cu (0, 15, 30, 60, 120 and 240 g Cu 100 kg-1 seed) and 4 seed-soaking time durations (0, 4, 8 and 16 h). The germination of wheat seed was 100% in the zero-Cu control treatments, irrespective of the duration of seed soaking time. However, seed germination decreased with increasing amount of fertilizer Cu in the seed-soaking solution, and the magnitude of reduction in seed germination due to Cu toxicity increased with increasing duration of seed-soaking time in the Cu fertilizer solution. For the seed-soaked treatments, the detrimental effect of Cu on germination was greatest with 16 h soaking, where only 13% - 18% of the seeds germinated with Cu applied at 15 to 30 g Cu 100 kg-1 seed. For the 4 and 8 h seed soaking treatments, germination of wheat seed ranged from 73% to 83% with 15 g Cu 100 kg-1 seed treatment and 42% to 62% with 30 g Cu 100 kg-1 seed. The findings suggest that the detrimental effect of Cu on germination of wheat seed soaked in Cu EDTA solution can be decreased by reducing duration of soaking time from 16 h to 4 or 8 h, but this needs further investigation using soil under growth chamber and/or field conditions in order to make valid recommendations for use of this new technology on a commercial scale.
文摘以常规粳稻秀水134为材料,设置清水浸种(CK)和咪酰胺浸种(Soak)两个处理,采用16S r RNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon指数、Chao1指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。
