目的探讨不同剂量^(60)Co-γ射线辐照灭菌对蜈蚣药粉8种核苷类成分含量及微生物指标的影响。方法分别以4,6,8,10 k Gy的^(60)Co-γ射线对样品进行动态辐照灭菌操作,按2015年版《中国药典(一部)》要求检测灭菌前后样品的水分、总灰分、...目的探讨不同剂量^(60)Co-γ射线辐照灭菌对蜈蚣药粉8种核苷类成分含量及微生物指标的影响。方法分别以4,6,8,10 k Gy的^(60)Co-γ射线对样品进行动态辐照灭菌操作,按2015年版《中国药典(一部)》要求检测灭菌前后样品的水分、总灰分、酸不溶性灰分、酸溶性浸出物、重金属及有害元素、黄曲霉毒素及微生物限度;采用高效液相色谱法测定4,6,8,10 k Gy^(60)Co-γ射线辐照灭菌后样品的化学成分含量。结果4,6,8,10 kGy^(60)Co-γ射线辐照灭菌样品的水分、总灰分、酸不溶性灰分、酸溶性浸出物、重金属及有害元素、黄曲霉毒素比较,差异均无统计学意义(P>0.05);大于6 kGy^(60)Co-γ射线辐照灭菌样品中,需氧菌、酵母菌、霉菌总数均随辐照剂量增加而减少,耐胆盐革兰阴性菌均小于10 cfu/g,微生物限度均符合2015年版《中国药典(四部)》规定,且均未检出大肠埃希菌和沙门菌;综合工业生产中的灭菌成本问题,8,10 kGy^(60)Co-γ射线动态辐照灭菌效果最佳;灭菌前后,胞嘧啶、胞苷、次黄嘌呤、黄嘌呤、尿苷、胸腺嘧啶、2’-脱氧肌苷、胸苷8种核苷类成分含量比较,差异均无统计学意义(P>0.05)。结论^(60)Co-γ射线辐照灭菌对蜈蚣药粉质量无明显影响,且8~10 kGy辐照灭菌效果最佳。展开更多
AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitonea...AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitoneally 1 h prior to the first injection of cerulein.Once AP developed,the stable cholecystokinin analogue,cerulein was injected hourly,over a 6 h period.Blood samples were taken 6 h later to determine serum amylase,lipase,and cytokine levels.The pancreas and lungs were rapidly removed for morphological examination,myeloperoxidase assay,and real-time reverse transcription polymerase chain reaction.To specify the role of SSM in pancreatitis,the pancreatic acinar cells were isolated using collagenase method.Then the cells were pre-treated with SSM,then stimulated with cerulein.The cell viability,cytokine productions and high-mobility group box protein-1(HMGB-1) were measured.Furthermore,the regulating mechanisms of SSM action were evaluated.RESULTS:The administration of SSM significantly attenuated the severity of pancreatitis and pancreatitis associated lung injury,as was shown by the reduction in pancreatic edema,neutrophil infiltration,vacuolization and necrosis.SSM treatment also reduced pancreatic weight/body weight ratio,serum amylase,lipase and cytokine levels,and mRNA expression of multiple inflammatory mediators such as tumor necrosis factor-α and interleukin-1β.In addition,treatment with SSM inhibited HMGB-1 expression in the pancreas during AP.In accordance with in vivo data,SSM inhibited the cerulein-induced acinar cell death,cytokine,and HMGB-1 release.SSM also inhibited the activation of c-Jun NH2-terminal kinase,p38 and nuclear factor(NF)-κB.CONCLUSION:These results suggest that SSM plays a protective role during the development of AP and pancreatitis associated lung injury via deactivating c-Jun NH2-terminal kinase,p38 and NF-κB.展开更多
文摘目的探讨不同剂量^(60)Co-γ射线辐照灭菌对蜈蚣药粉8种核苷类成分含量及微生物指标的影响。方法分别以4,6,8,10 k Gy的^(60)Co-γ射线对样品进行动态辐照灭菌操作,按2015年版《中国药典(一部)》要求检测灭菌前后样品的水分、总灰分、酸不溶性灰分、酸溶性浸出物、重金属及有害元素、黄曲霉毒素及微生物限度;采用高效液相色谱法测定4,6,8,10 k Gy^(60)Co-γ射线辐照灭菌后样品的化学成分含量。结果4,6,8,10 kGy^(60)Co-γ射线辐照灭菌样品的水分、总灰分、酸不溶性灰分、酸溶性浸出物、重金属及有害元素、黄曲霉毒素比较,差异均无统计学意义(P>0.05);大于6 kGy^(60)Co-γ射线辐照灭菌样品中,需氧菌、酵母菌、霉菌总数均随辐照剂量增加而减少,耐胆盐革兰阴性菌均小于10 cfu/g,微生物限度均符合2015年版《中国药典(四部)》规定,且均未检出大肠埃希菌和沙门菌;综合工业生产中的灭菌成本问题,8,10 kGy^(60)Co-γ射线动态辐照灭菌效果最佳;灭菌前后,胞嘧啶、胞苷、次黄嘌呤、黄嘌呤、尿苷、胸腺嘧啶、2’-脱氧肌苷、胸苷8种核苷类成分含量比较,差异均无统计学意义(P>0.05)。结论^(60)Co-γ射线辐照灭菌对蜈蚣药粉质量无明显影响,且8~10 kGy辐照灭菌效果最佳。
基金Supported by National Research Foundation of Korea grant funded by the Korea government MEST,No. 2010-0029498
文摘AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitoneally 1 h prior to the first injection of cerulein.Once AP developed,the stable cholecystokinin analogue,cerulein was injected hourly,over a 6 h period.Blood samples were taken 6 h later to determine serum amylase,lipase,and cytokine levels.The pancreas and lungs were rapidly removed for morphological examination,myeloperoxidase assay,and real-time reverse transcription polymerase chain reaction.To specify the role of SSM in pancreatitis,the pancreatic acinar cells were isolated using collagenase method.Then the cells were pre-treated with SSM,then stimulated with cerulein.The cell viability,cytokine productions and high-mobility group box protein-1(HMGB-1) were measured.Furthermore,the regulating mechanisms of SSM action were evaluated.RESULTS:The administration of SSM significantly attenuated the severity of pancreatitis and pancreatitis associated lung injury,as was shown by the reduction in pancreatic edema,neutrophil infiltration,vacuolization and necrosis.SSM treatment also reduced pancreatic weight/body weight ratio,serum amylase,lipase and cytokine levels,and mRNA expression of multiple inflammatory mediators such as tumor necrosis factor-α and interleukin-1β.In addition,treatment with SSM inhibited HMGB-1 expression in the pancreas during AP.In accordance with in vivo data,SSM inhibited the cerulein-induced acinar cell death,cytokine,and HMGB-1 release.SSM also inhibited the activation of c-Jun NH2-terminal kinase,p38 and nuclear factor(NF)-κB.CONCLUSION:These results suggest that SSM plays a protective role during the development of AP and pancreatitis associated lung injury via deactivating c-Jun NH2-terminal kinase,p38 and NF-κB.
