Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicide-resistant transgenic perennial ryegrass （Lolium perenne L.） was obtained by transforming it with ...Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicide-resistant transgenic perennial ryegrass （Lolium perenne L.） was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene （cry8Ca2 or cry8Ga） and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta, while leaves of the wild plants wilted after Basta spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants.展开更多
Prophaenognatha robusta gen.et sp.nov.(Scarabaeoidea:Scarabaeidae:Aclopinae),the best-preserved aclopine fossil so far,is described and illustrated from the Upper Jurassic-Lower Cretaceous Yixian Formation of the Jeho...Prophaenognatha robusta gen.et sp.nov.(Scarabaeoidea:Scarabaeidae:Aclopinae),the best-preserved aclopine fossil so far,is described and illustrated from the Upper Jurassic-Lower Cretaceous Yixian Formation of the Jehol Biota,western Liaoning Province,NE China.The key to extinct and extant genera of Aclopinae is given and the monophyly of extant and fossil Aclopinae lineages is supported by five character states.The new taxon provides evidence about the evolution of Scarabaeoidea with its phylogenetic position inferred based on 68 morphological characters.展开更多
Cuticles of some Chrysina scarabs are characterized by flat, graded, and twisted structures of nanosized chitin fibrils. As inferred from SEM images, each species has its own spatial period or pitch P which is depende...Cuticles of some Chrysina scarabs are characterized by flat, graded, and twisted structures of nanosized chitin fibrils. As inferred from SEM images, each species has its own spatial period or pitch P which is dependent on the depth z through the cuticle. From Berreman’s formalism, taking into account the corresponding P(z) dependence, we evaluate reflection spectra of C. aurigans and C. chrysargyrea scarabs. The spectra display the main spectral features observed in the measured ones when small sections of the cuticles are illuminated with non-polarized light, for wavelengths between 300 and 1100 nm. By considering these twisted structures as 1D photonic crystals, an approach is developed to show how the broad band characterizing the reflection spectra arises from a narrow intrinsic photonic band width, whose spectral position moves through visible and near infrared wavelengths. The role of the epicuticle that covers the twisted structures is analyzed in terms of a waxy layer acting as an anti-reflecting coating that also shows low levels of light scattering.展开更多
基金supported by the National Basic Research Program of China (973 Program,2007CB1089).
文摘Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicide-resistant transgenic perennial ryegrass （Lolium perenne L.） was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene （cry8Ca2 or cry8Ga） and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta, while leaves of the wild plants wilted after Basta spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants.
基金supported by the National Basic Research Program of China(973 Program)(No. 2011CB302102)the National Natural Science Foundation of China(Nos.30900144,31071964,40872022 and 31010103913)+2 种基金the Knowledge Innovation Program of Chinese Academy of Sciences(Nos.KSCX2-EW-G-4, KSCX2-EW-Z-8 and KSCX3-IOZ-1004)the Scientific Research Key Program(KZ200910028005)the PHR20090509,201107120 Project of Beijing Municipal Commission of Education
文摘Prophaenognatha robusta gen.et sp.nov.(Scarabaeoidea:Scarabaeidae:Aclopinae),the best-preserved aclopine fossil so far,is described and illustrated from the Upper Jurassic-Lower Cretaceous Yixian Formation of the Jehol Biota,western Liaoning Province,NE China.The key to extinct and extant genera of Aclopinae is given and the monophyly of extant and fossil Aclopinae lineages is supported by five character states.The new taxon provides evidence about the evolution of Scarabaeoidea with its phylogenetic position inferred based on 68 morphological characters.
文摘Cuticles of some Chrysina scarabs are characterized by flat, graded, and twisted structures of nanosized chitin fibrils. As inferred from SEM images, each species has its own spatial period or pitch P which is dependent on the depth z through the cuticle. From Berreman’s formalism, taking into account the corresponding P(z) dependence, we evaluate reflection spectra of C. aurigans and C. chrysargyrea scarabs. The spectra display the main spectral features observed in the measured ones when small sections of the cuticles are illuminated with non-polarized light, for wavelengths between 300 and 1100 nm. By considering these twisted structures as 1D photonic crystals, an approach is developed to show how the broad band characterizing the reflection spectra arises from a narrow intrinsic photonic band width, whose spectral position moves through visible and near infrared wavelengths. The role of the epicuticle that covers the twisted structures is analyzed in terms of a waxy layer acting as an anti-reflecting coating that also shows low levels of light scattering.
