The changes of sclenium metabolism, glutathione peroxidase activity and lipid peroxidescontent in the tissues of rats suffering from 30% TBSA full thickness scalding were observed in thefirst 7 days after injury. It w...The changes of sclenium metabolism, glutathione peroxidase activity and lipid peroxidescontent in the tissues of rats suffering from 30% TBSA full thickness scalding were observed in thefirst 7 days after injury. It was found that selenium content in the rat tissues decreased remarkably af-ter injury, which in turn resulted in serious reduction of glutathione peroxidasc activity and significantincrease of lipid peroxides in the scrum, crythrocytcs and liver. However the muscular tissue showedno significant changes. These facts imply that after burn injury, the body is in a state of selenium deficiency, the lossof selenium might be responsible for the reduction of anti - peroxidation ability of glutathioneperoxidase, and conscqucntly there is an increase of lipid peroxides in the tissues. Only the musculartissue is insensitive to lipid peroxidation. It is believed that the reduction of anti-peroxidation abilityof glutathione peroxidasc after bum injury might be one of the main causes to intensify, the injury re-suiting from free radicals.展开更多
Scald injuries,which describe burns to living tissue from hot liquids,are a very common injury that occur across geographical,social,economic,and national boundaries.Despite their ubiquitous nature,a complete understa...Scald injuries,which describe burns to living tissue from hot liquids,are a very common injury that occur across geographical,social,economic,and national boundaries.Despite their ubiquitous nature,a complete understanding of the conditions which are required to cause scald burns is not yet available.In addition,clear guidance to medical practitioners is available through various guidelines however in actual situations,the extent of the burn is not fully known and this lack of knowledge complicates care.Here,a comprehensive review is made of the available knowledge of temperatures and scald durations which lead to skin-burn injuries.The range of volumes and liquid temperatures are typical of those found in heated consumer beverages.This review can help medical practitioners design initial treatment protocols and can be used by manufacturers of hot-liquid products to avoid the most severe burns.Next,within the context of this ability to quantify burn depths,a review of current burn treatment guidelines is given.Included in this review is a visual recognition of the extent of burns into the dermal layer as well as decision guidelines for selection of patients which would benefit from referral to a dedicated burn center.It is hoped that by bringing together both the quantified burn-depth information and current treatment guidelines,this review can be used as a resource for persons in the medical,manufacturing,beverage service,and other industries to reduce the human impact of scald injuries.展开更多
目的探讨300 mmol/L(mM)高渗盐溶液(HS)对重度烫伤大鼠肾损伤的影响。方法按照随机数表法将56只大鼠随机分为假伤组(8只)、LR组(24只)与HS组(24只),其中假伤组大鼠做假伤处理后直接处死,LR组大鼠建立重度烫伤模型后予以乳酸钠林格注射液...目的探讨300 mmol/L(mM)高渗盐溶液(HS)对重度烫伤大鼠肾损伤的影响。方法按照随机数表法将56只大鼠随机分为假伤组(8只)、LR组(24只)与HS组(24只),其中假伤组大鼠做假伤处理后直接处死,LR组大鼠建立重度烫伤模型后予以乳酸钠林格注射液(LR)复苏治疗,HS组大鼠建立重度烫伤模型后予以300 mM HS(1 L 300 mM HS含97 mL 10%NaCl和903 mL LR)复苏治疗,对比观察各组大鼠肾脏组织湿干重比值(W/D)、p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinases,p38 MAPK)通道活化程度及血清肌酐(Cr)、中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase associated lipocalin,NGAL)、肿瘤坏死因子 α(TNF α)、白细胞介素 6(IL 6)、高迁移率族蛋白B1(highmobility group box1,HMGB1)的水平变化。结果与假伤组对比,LR组及HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度及血清Cr、NGAL、TNF α、IL 6水平,以及伤后8、24 h HMGB1水平均较高,P均<0.05,差异具有统计学意义;而伤后2 h HMGB1水平与假伤组相同,P>0.05,差异无统计学意义。与LR组对比,HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度均较低,P均<0.05,差异具有统计学意义;HS组大鼠伤后2 h血清Cr、HMGB1水平与LR组基本相同,P均>0.05,差异无统计学意义,而伤后8、24 h血清Cr、HMGB1水平均低于LR组,P均<0.05,差异具有统计学意义;HS组大鼠伤后2、8、24 h血清NGAL、TNF α、IL 6水平均低于LR组,P均<0.05,差异具有统计学意义。结论HS可通过抑制血清炎症介质的产生及下调p38 MAPK信号通路活化程度而减轻肾脏的损伤程度,故烧伤后可早期应用HS予以复苏抗休克。展开更多
目的观察丙酮酸乙酯(ethyl pyruvate,EP)对烫伤大鼠肺组织高迁移率族蛋白B1(high mobility group box-1protein,HMGB1)表达水平及急性肺损伤的影响。方法采用大鼠30%总体表面积Ⅲ度烫伤延迟复苏模型,78只雄性Wistar大鼠按随机数字表法...目的观察丙酮酸乙酯(ethyl pyruvate,EP)对烫伤大鼠肺组织高迁移率族蛋白B1(high mobility group box-1protein,HMGB1)表达水平及急性肺损伤的影响。方法采用大鼠30%总体表面积Ⅲ度烫伤延迟复苏模型,78只雄性Wistar大鼠按随机数字表法分为假烫伤组(n=18)、烫伤组(n=30)、EP治疗组(n=30),每组分别在假伤或伤后第8、24、72小时活杀留取肺组织。HMGB1基因/蛋白表达分别采用逆转录聚合酶链反应及蛋白免疫印记法、免疫组化方法检测;髓过氧化物酶(myelope roxidase,MPO)活性采用酶学分光光度法测定;并采用HE染色、光镜下观察肺组织病理改变。结果与假烫伤组比较,烫伤组肺组织HMGB1基因/蛋白表达于伤后8～72h显著增强(P<0.05,P<0.01),同时肺组织MPO活性在8h及24h明显升高(P<0.01),病理学观察见肺组织炎细胞浸润,正常结构破坏,其中以24h改变最重。与烫伤组比较,EP治疗组大鼠肺组织8～72h时间点HMGB1表达显著下调(P<0.05),肺组织MPO活性在8、24h时间点显著下降(P<0.01),EP治疗组8～72h肺组织病理形态损害明显减轻。结论HMGB1作为晚期炎症因子参与了烫伤后肺组织炎症反应的病理过程,EP治疗可明显下调肺组织HMGB1表达,并有助于降低肺组织MPO活性,从而减轻烫伤延迟复苏所致急性肺损伤。展开更多
文摘The changes of sclenium metabolism, glutathione peroxidase activity and lipid peroxidescontent in the tissues of rats suffering from 30% TBSA full thickness scalding were observed in thefirst 7 days after injury. It was found that selenium content in the rat tissues decreased remarkably af-ter injury, which in turn resulted in serious reduction of glutathione peroxidasc activity and significantincrease of lipid peroxides in the scrum, crythrocytcs and liver. However the muscular tissue showedno significant changes. These facts imply that after burn injury, the body is in a state of selenium deficiency, the lossof selenium might be responsible for the reduction of anti - peroxidation ability of glutathioneperoxidase, and conscqucntly there is an increase of lipid peroxides in the tissues. Only the musculartissue is insensitive to lipid peroxidation. It is believed that the reduction of anti-peroxidation abilityof glutathione peroxidasc after bum injury might be one of the main causes to intensify, the injury re-suiting from free radicals.
文摘Scald injuries,which describe burns to living tissue from hot liquids,are a very common injury that occur across geographical,social,economic,and national boundaries.Despite their ubiquitous nature,a complete understanding of the conditions which are required to cause scald burns is not yet available.In addition,clear guidance to medical practitioners is available through various guidelines however in actual situations,the extent of the burn is not fully known and this lack of knowledge complicates care.Here,a comprehensive review is made of the available knowledge of temperatures and scald durations which lead to skin-burn injuries.The range of volumes and liquid temperatures are typical of those found in heated consumer beverages.This review can help medical practitioners design initial treatment protocols and can be used by manufacturers of hot-liquid products to avoid the most severe burns.Next,within the context of this ability to quantify burn depths,a review of current burn treatment guidelines is given.Included in this review is a visual recognition of the extent of burns into the dermal layer as well as decision guidelines for selection of patients which would benefit from referral to a dedicated burn center.It is hoped that by bringing together both the quantified burn-depth information and current treatment guidelines,this review can be used as a resource for persons in the medical,manufacturing,beverage service,and other industries to reduce the human impact of scald injuries.
文摘目的探讨300 mmol/L(mM)高渗盐溶液(HS)对重度烫伤大鼠肾损伤的影响。方法按照随机数表法将56只大鼠随机分为假伤组(8只)、LR组(24只)与HS组(24只),其中假伤组大鼠做假伤处理后直接处死,LR组大鼠建立重度烫伤模型后予以乳酸钠林格注射液(LR)复苏治疗,HS组大鼠建立重度烫伤模型后予以300 mM HS(1 L 300 mM HS含97 mL 10%NaCl和903 mL LR)复苏治疗,对比观察各组大鼠肾脏组织湿干重比值(W/D)、p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinases,p38 MAPK)通道活化程度及血清肌酐(Cr)、中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase associated lipocalin,NGAL)、肿瘤坏死因子 α(TNF α)、白细胞介素 6(IL 6)、高迁移率族蛋白B1(highmobility group box1,HMGB1)的水平变化。结果与假伤组对比,LR组及HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度及血清Cr、NGAL、TNF α、IL 6水平,以及伤后8、24 h HMGB1水平均较高,P均<0.05,差异具有统计学意义;而伤后2 h HMGB1水平与假伤组相同,P>0.05,差异无统计学意义。与LR组对比,HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度均较低,P均<0.05,差异具有统计学意义;HS组大鼠伤后2 h血清Cr、HMGB1水平与LR组基本相同,P均>0.05,差异无统计学意义,而伤后8、24 h血清Cr、HMGB1水平均低于LR组,P均<0.05,差异具有统计学意义;HS组大鼠伤后2、8、24 h血清NGAL、TNF α、IL 6水平均低于LR组,P均<0.05,差异具有统计学意义。结论HS可通过抑制血清炎症介质的产生及下调p38 MAPK信号通路活化程度而减轻肾脏的损伤程度,故烧伤后可早期应用HS予以复苏抗休克。
