Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as...Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.展开更多
In this paper,we propose and analyze high order energy dissipative time-stepping schemes for time-fractional molecular beam epitaxial(MBE)growth model on the nonuniform mesh.More precisely,(2−α)-order,secondorder and...In this paper,we propose and analyze high order energy dissipative time-stepping schemes for time-fractional molecular beam epitaxial(MBE)growth model on the nonuniform mesh.More precisely,(2−α)-order,secondorder and(3−α)-order time-stepping schemes are developed for the timefractional MBE model based on the well known L1,L2-1σ,and L2 formulations in discretization of the time-fractional derivative,which are all proved to be unconditional energy dissipation in the sense of a modified discrete nonlocalenergy on the nonuniform mesh.In order to reduce the computational storage,we apply the sum of exponential technique to approximate the history part of the time-fractional derivative.Moreover,the scalar auxiliary variable(SAV)approach is introduced to deal with the nonlinear potential function and the history part of the fractional derivative.Furthermore,only first order method is used to discretize the introduced SAV equation,which will not affect high order accuracy of the unknown thin film height function by using some proper auxiliary variable functions V(ξ).To our knowledge,it is the first time to unconditionally establish the discrete nonlocal-energy dissipation law for the modified L1-,L2-1σ-,and L2-based high-order schemes on the nonuniform mesh,which is essentially important for such time-fractional MBE models with low regular solutions at initial time.Finally,a series of numerical experiments are carried out to verify the accuracy and efficiency of the proposed schemes.展开更多
SAV1 is a core component involved in the Hippo pathway which can control the organ size via regulating cell proliferation and apoptosis simultaneously. We explored the regulatory mechanism of SAV1. We established the ...SAV1 is a core component involved in the Hippo pathway which can control the organ size via regulating cell proliferation and apoptosis simultaneously. We explored the regulatory mechanism of SAV1. We established the HEK293T cell pool, the cells in which can stably express SAV1 by retroviruses infection and found that SAV1 stable cells reduced the movement of themselves and resulted in multicellular aggregation. We purified SAV1 interacting protein complex using streptavidin resin and subsequently analyzed the digested peptides by high performance liquid chromatography（HPLC）-MS/MS. Results show that about 150 proteins were identified in the complex of SAV 1 with protein. TUBA1A, OTUD4, and ATD were identified as proteins interacting with SAV1. Importantly, PP1A, se- rine/threonine protein phosphatase PPl-alpha 1 catalytic subunit, was also in the top 10 list. The interaction between PP1A and SAV1 was detected by both co-immunoprecipitation（CO-IP） and immunostaining. Our results indicate that PP1A might be the phosphatase of SAV1 and may take part in the regulation of the Hippo pathway.展开更多
基金The National Natural Science Foundation of China (81473321)Beijing Municipal Natural Science Foundation (7152086)+1 种基金the National Key Technology R & D Program of China (2011BAI07B082012BAI29B02)
文摘Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.
基金supported by NSFC grant 12001248,the NSF of Jiangsu Province grant BK20201020the NSF of Universities in Jiangsu Province of China grant 20KJB110013+3 种基金the Hong Kong Polytechnic University grant 1-W00Dsupported by Hong Kong Research Grants Council RFS grant RFS2021-5S03 and GRF grant 15302122,the Hong Kong Polytechnic University grant 1-9BCTCAS AMSS-PolyU Joint Laboratory of Applied Mathematicssupported by the Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science under UIC 2022B1212010006.
文摘In this paper,we propose and analyze high order energy dissipative time-stepping schemes for time-fractional molecular beam epitaxial(MBE)growth model on the nonuniform mesh.More precisely,(2−α)-order,secondorder and(3−α)-order time-stepping schemes are developed for the timefractional MBE model based on the well known L1,L2-1σ,and L2 formulations in discretization of the time-fractional derivative,which are all proved to be unconditional energy dissipation in the sense of a modified discrete nonlocalenergy on the nonuniform mesh.In order to reduce the computational storage,we apply the sum of exponential technique to approximate the history part of the time-fractional derivative.Moreover,the scalar auxiliary variable(SAV)approach is introduced to deal with the nonlinear potential function and the history part of the fractional derivative.Furthermore,only first order method is used to discretize the introduced SAV equation,which will not affect high order accuracy of the unknown thin film height function by using some proper auxiliary variable functions V(ξ).To our knowledge,it is the first time to unconditionally establish the discrete nonlocal-energy dissipation law for the modified L1-,L2-1σ-,and L2-based high-order schemes on the nonuniform mesh,which is essentially important for such time-fractional MBE models with low regular solutions at initial time.Finally,a series of numerical experiments are carried out to verify the accuracy and efficiency of the proposed schemes.
基金Supported by the National Natural Science Foundation of China（No.30973274） and the Doctoral Fund of Youth Scholars of the Ministry of Education of China（No.20090061120073）.
文摘SAV1 is a core component involved in the Hippo pathway which can control the organ size via regulating cell proliferation and apoptosis simultaneously. We explored the regulatory mechanism of SAV1. We established the HEK293T cell pool, the cells in which can stably express SAV1 by retroviruses infection and found that SAV1 stable cells reduced the movement of themselves and resulted in multicellular aggregation. We purified SAV1 interacting protein complex using streptavidin resin and subsequently analyzed the digested peptides by high performance liquid chromatography（HPLC）-MS/MS. Results show that about 150 proteins were identified in the complex of SAV 1 with protein. TUBA1A, OTUD4, and ATD were identified as proteins interacting with SAV1. Importantly, PP1A, se- rine/threonine protein phosphatase PPl-alpha 1 catalytic subunit, was also in the top 10 list. The interaction between PP1A and SAV1 was detected by both co-immunoprecipitation（CO-IP） and immunostaining. Our results indicate that PP1A might be the phosphatase of SAV1 and may take part in the regulation of the Hippo pathway.
